Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.

Detalhes bibliográficos
Autor(a) principal: FRAGA, H. P. de F.
Data de Publicação: 2016
Outros Autores: VIEIRA, L. do N., PUTTKAMMER, C. C., SANTOS, H. P. dos, GARIGHAN, J. de A., GUERRA, M. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054895
http://dx.doi.org/10.1016/j.plantsci.2016.09.012
Resumo: Here we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was signifi- cant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0 mM and GSH 0.1 mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5 mM treatment showed constantlevels. Alltreatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5 mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesized
id EMBR_9e19e2d1477613c91ff2c4a29ca37ba8
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/1054895
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.Brazilian coniferRedox metabolismEmbryonic developmentMicropropagationIn vitro cultureHere we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was signifi- cant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0 mM and GSH 0.1 mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5 mM treatment showed constantlevels. Alltreatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5 mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesizedHugo pacheco de Freitas Fraga, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Leila do Nascimento Vieira, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Catarina Corrêa Puttkammer, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; HENRIQUE PESSOA DOS SANTOS, CNPUV; Julio de Andrade Garighan, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Miguel PEdro Guerra, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil.FRAGA, H. P. de F.VIEIRA, L. do N.PUTTKAMMER, C. C.SANTOS, H. P. dosGARIGHAN, J. de A.GUERRA, M. P.2016-10-18T11:11:11Z2016-10-18T11:11:11Z2016-10-1820162019-05-06T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Science, n. 235, p. 98-106, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054895http://dx.doi.org/10.1016/j.plantsci.2016.09.012enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:31:31Zoai:www.alice.cnptia.embrapa.br:doc/1054895Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:31:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:31:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
title Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
spellingShingle Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
FRAGA, H. P. de F.
Brazilian conifer
Redox metabolism
Embryonic development
Micropropagation
In vitro culture
title_short Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
title_full Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
title_fullStr Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
title_full_unstemmed Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
title_sort Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
author FRAGA, H. P. de F.
author_facet FRAGA, H. P. de F.
VIEIRA, L. do N.
PUTTKAMMER, C. C.
SANTOS, H. P. dos
GARIGHAN, J. de A.
GUERRA, M. P.
author_role author
author2 VIEIRA, L. do N.
PUTTKAMMER, C. C.
SANTOS, H. P. dos
GARIGHAN, J. de A.
GUERRA, M. P.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Hugo pacheco de Freitas Fraga, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Leila do Nascimento Vieira, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Catarina Corrêa Puttkammer, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; HENRIQUE PESSOA DOS SANTOS, CNPUV; Julio de Andrade Garighan, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil; Miguel PEdro Guerra, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-001, Brazil.
dc.contributor.author.fl_str_mv FRAGA, H. P. de F.
VIEIRA, L. do N.
PUTTKAMMER, C. C.
SANTOS, H. P. dos
GARIGHAN, J. de A.
GUERRA, M. P.
dc.subject.por.fl_str_mv Brazilian conifer
Redox metabolism
Embryonic development
Micropropagation
In vitro culture
topic Brazilian conifer
Redox metabolism
Embryonic development
Micropropagation
In vitro culture
description Here we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was signifi- cant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0 mM and GSH 0.1 mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5 mM treatment showed constantlevels. Alltreatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5 mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesized
publishDate 2016
dc.date.none.fl_str_mv 2016-10-18T11:11:11Z
2016-10-18T11:11:11Z
2016-10-18
2016
2019-05-06T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plant Science, n. 235, p. 98-106, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054895
http://dx.doi.org/10.1016/j.plantsci.2016.09.012
identifier_str_mv Plant Science, n. 235, p. 98-106, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054895
http://dx.doi.org/10.1016/j.plantsci.2016.09.012
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503426978086912

Registros relacionados