Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.

Detalhes bibliográficos
Autor(a) principal: SOUZA, S. C. de
Data de Publicação: 2018
Outros Autores: CAVALCANTI, J. J. V., RAMOS, J. P. C., ALVES, I., SANTOS, R. C. dos, LIMA, L. M. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099578
Resumo: The Agave genus is composed of about 200 species, but the cultivation of sisal for fiber production in Brazil is restricted to two species: Agave sisalana and Agave fourcroydes, both have several accessions with wide variability. The collection of Agave of Embrapa has 37 accessions maintained in situ and periodically evaluated agronomical traits. Most of these accessions have phenotypic similarities, although they differ in fiber quality, which are widely used for commercial purposes. The identification of promising accesses contributes to the advance in improvement works, focusing on commercial indication. In order to estimate the genetic divergence of this collection, a cluster analyses was performed based on Inter-Simple Sequence Repeat (ISSR) markers and phenotypic traits. Genomic DNA from these accessions were used in polymerase chain reaction (PCR) with thirty ISSR oligonucleotides. For phenotypic characterization, twelve descriptors were adopted based on morphological and agronomic data. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Tocher multivariate methods were adopted. Thirteen groups were formed by the Tocher Method and six by UPGMA; however UPGMA method was more representative in the group formation. The comparison of the band patterns among accessions derived from the shoots showed that genetic variability is generated during asexual reproduction in these plants. The four lines generated from Tatui were the most divergent accessions. These plants are tallest, with higher mass values of fresh and dry mucilage, fresh and dry fiber mass, fiber length and presence of spines at the edges. The accessions from Instituto Agronômico de Campinas (IAC) showed the lowest genetic distances, indicating a possible narrow genetic base and high kinship degree. The crossings between H-RN, H-Kenya, H-400 fls, and H-11648 with Tatuí 1, 2, 3, and 4 can be a valorous strategy to broadening genetic diversity among commercial and native sisal germplasm.
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spelling Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.Marcador MolecularAgave SisalanaSisalMelhoramento Genético VegetalBanco de GermoplasmaFibra VegetalGermplasmThe Agave genus is composed of about 200 species, but the cultivation of sisal for fiber production in Brazil is restricted to two species: Agave sisalana and Agave fourcroydes, both have several accessions with wide variability. The collection of Agave of Embrapa has 37 accessions maintained in situ and periodically evaluated agronomical traits. Most of these accessions have phenotypic similarities, although they differ in fiber quality, which are widely used for commercial purposes. The identification of promising accesses contributes to the advance in improvement works, focusing on commercial indication. In order to estimate the genetic divergence of this collection, a cluster analyses was performed based on Inter-Simple Sequence Repeat (ISSR) markers and phenotypic traits. Genomic DNA from these accessions were used in polymerase chain reaction (PCR) with thirty ISSR oligonucleotides. For phenotypic characterization, twelve descriptors were adopted based on morphological and agronomic data. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Tocher multivariate methods were adopted. Thirteen groups were formed by the Tocher Method and six by UPGMA; however UPGMA method was more representative in the group formation. The comparison of the band patterns among accessions derived from the shoots showed that genetic variability is generated during asexual reproduction in these plants. The four lines generated from Tatui were the most divergent accessions. These plants are tallest, with higher mass values of fresh and dry mucilage, fresh and dry fiber mass, fiber length and presence of spines at the edges. The accessions from Instituto Agronômico de Campinas (IAC) showed the lowest genetic distances, indicating a possible narrow genetic base and high kinship degree. The crossings between H-RN, H-Kenya, H-400 fls, and H-11648 with Tatuí 1, 2, 3, and 4 can be a valorous strategy to broadening genetic diversity among commercial and native sisal germplasm.Article number: 4C7CCF056288SILMARA CHAVES DE SOUZA, UEPB - CAMPINA GRANDE, PB; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; JEAN PIERRE CORDEIRO RAMOS, UFPB - CAMPUS II , AREIA; ISAIAS ALVES, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA; LIZIANE MARIA DE LIMA, CNPA.SOUZA, S. C. deCAVALCANTI, J. J. V.RAMOS, J. P. C.ALVES, I.SANTOS, R. C. dosLIMA, L. M. de2018-11-23T23:46:32Z2018-11-23T23:46:32Z2018-11-1920182018-11-23T23:46:32Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAfrican Journal of Agricultural Research, v. 13, n. 11, p. 526-533, Mar. 2018.1991-637Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/109957810.5897/AJAR2017.12913enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-11-23T23:46:37Zoai:www.alice.cnptia.embrapa.br:doc/1099578Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-11-23T23:46:37falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-11-23T23:46:37Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
title Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
spellingShingle Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
SOUZA, S. C. de
Marcador Molecular
Agave Sisalana
Sisal
Melhoramento Genético Vegetal
Banco de Germoplasma
Fibra Vegetal
Germplasm
title_short Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
title_full Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
title_fullStr Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
title_full_unstemmed Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
title_sort Genetic divergence in Agave accessions through ISSR markers and phenotypic traits.
author SOUZA, S. C. de
author_facet SOUZA, S. C. de
CAVALCANTI, J. J. V.
RAMOS, J. P. C.
ALVES, I.
SANTOS, R. C. dos
LIMA, L. M. de
author_role author
author2 CAVALCANTI, J. J. V.
RAMOS, J. P. C.
ALVES, I.
SANTOS, R. C. dos
LIMA, L. M. de
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv SILMARA CHAVES DE SOUZA, UEPB - CAMPINA GRANDE, PB; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; JEAN PIERRE CORDEIRO RAMOS, UFPB - CAMPUS II , AREIA; ISAIAS ALVES, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA; LIZIANE MARIA DE LIMA, CNPA.
dc.contributor.author.fl_str_mv SOUZA, S. C. de
CAVALCANTI, J. J. V.
RAMOS, J. P. C.
ALVES, I.
SANTOS, R. C. dos
LIMA, L. M. de
dc.subject.por.fl_str_mv Marcador Molecular
Agave Sisalana
Sisal
Melhoramento Genético Vegetal
Banco de Germoplasma
Fibra Vegetal
Germplasm
topic Marcador Molecular
Agave Sisalana
Sisal
Melhoramento Genético Vegetal
Banco de Germoplasma
Fibra Vegetal
Germplasm
description The Agave genus is composed of about 200 species, but the cultivation of sisal for fiber production in Brazil is restricted to two species: Agave sisalana and Agave fourcroydes, both have several accessions with wide variability. The collection of Agave of Embrapa has 37 accessions maintained in situ and periodically evaluated agronomical traits. Most of these accessions have phenotypic similarities, although they differ in fiber quality, which are widely used for commercial purposes. The identification of promising accesses contributes to the advance in improvement works, focusing on commercial indication. In order to estimate the genetic divergence of this collection, a cluster analyses was performed based on Inter-Simple Sequence Repeat (ISSR) markers and phenotypic traits. Genomic DNA from these accessions were used in polymerase chain reaction (PCR) with thirty ISSR oligonucleotides. For phenotypic characterization, twelve descriptors were adopted based on morphological and agronomic data. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Tocher multivariate methods were adopted. Thirteen groups were formed by the Tocher Method and six by UPGMA; however UPGMA method was more representative in the group formation. The comparison of the band patterns among accessions derived from the shoots showed that genetic variability is generated during asexual reproduction in these plants. The four lines generated from Tatui were the most divergent accessions. These plants are tallest, with higher mass values of fresh and dry mucilage, fresh and dry fiber mass, fiber length and presence of spines at the edges. The accessions from Instituto Agronômico de Campinas (IAC) showed the lowest genetic distances, indicating a possible narrow genetic base and high kinship degree. The crossings between H-RN, H-Kenya, H-400 fls, and H-11648 with Tatuí 1, 2, 3, and 4 can be a valorous strategy to broadening genetic diversity among commercial and native sisal germplasm.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-23T23:46:32Z
2018-11-23T23:46:32Z
2018-11-19
2018
2018-11-23T23:46:32Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv African Journal of Agricultural Research, v. 13, n. 11, p. 526-533, Mar. 2018.
1991-637X
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099578
10.5897/AJAR2017.12913
identifier_str_mv African Journal of Agricultural Research, v. 13, n. 11, p. 526-533, Mar. 2018.
1991-637X
10.5897/AJAR2017.12913
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099578
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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