Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113699 |
Resumo: | The peanut plant has high plasticity and great adaptability to adverse conditions, including drought. To mitigate the negative effects of drought on legumes, nitrogen-fixing microorganisms have been investigated in some plant species, such as soybeans and beans. We analyzed the role Bradyrhizobium strains in peanut genotypes subjected to water deficit by means of plant growth, physiological and gene expression analysis. The research was conducted under greenhouse conditions with the runner peanut genotypes (IAC Runner 886, 2012-33 and 2012-47) and two Bradyrhizobium strains (ESA 123 and SEMIA 6144). After 20 days of germination, the water supply was completely interrupted and gas exchange analysis were carried out using an infrared gas analyzer, up to the 10th day of stress. Leaves were collected for the analysis of proline content and the expression of NCED and ERF8 genes. Analyses of plant height (PH), shoot dry mass (SDM), root dry mass (RDM), number of nodules (NN) and nodule weight (NW) also were performed. The peanut shoots inoculated with the Bradyrhizobium strains obtained the best results. The genotypes inoculated with the ESA 123 strain obtained superior responses compared to the nonstressed treatment inoculated with the same bacteria and the stressed control without bacterial inoculation. In the ESA 123 inoculated treatments, the water stressed plants had higher RDM (28.5% higher, on average), NN (two fold higher), and gene expression (approximately six and three-fold higher for ERF8 and NCED genes, respectively). The increase in the expression of NCED and ERF8 genes, in the three genotypes inoculated with ESA 123, suggests a key role of this inoculant in the activation of metabolic cascades for plant protection under water deficit. |
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Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses.Fixação biológica de nitrogênioGenes ERF8 e NCEDTolerância à secaArachis hipogaeaFixação de NitrogênioAmendoimLeguminosaGenótipoPeanutsBradyrhizobiumNitrogen fixationThe peanut plant has high plasticity and great adaptability to adverse conditions, including drought. To mitigate the negative effects of drought on legumes, nitrogen-fixing microorganisms have been investigated in some plant species, such as soybeans and beans. We analyzed the role Bradyrhizobium strains in peanut genotypes subjected to water deficit by means of plant growth, physiological and gene expression analysis. The research was conducted under greenhouse conditions with the runner peanut genotypes (IAC Runner 886, 2012-33 and 2012-47) and two Bradyrhizobium strains (ESA 123 and SEMIA 6144). After 20 days of germination, the water supply was completely interrupted and gas exchange analysis were carried out using an infrared gas analyzer, up to the 10th day of stress. Leaves were collected for the analysis of proline content and the expression of NCED and ERF8 genes. Analyses of plant height (PH), shoot dry mass (SDM), root dry mass (RDM), number of nodules (NN) and nodule weight (NW) also were performed. The peanut shoots inoculated with the Bradyrhizobium strains obtained the best results. The genotypes inoculated with the ESA 123 strain obtained superior responses compared to the nonstressed treatment inoculated with the same bacteria and the stressed control without bacterial inoculation. In the ESA 123 inoculated treatments, the water stressed plants had higher RDM (28.5% higher, on average), NN (two fold higher), and gene expression (approximately six and three-fold higher for ERF8 and NCED genes, respectively). The increase in the expression of NCED and ERF8 genes, in the three genotypes inoculated with ESA 123, suggests a key role of this inoculant in the activation of metabolic cascades for plant protection under water deficit.S. L. BRITO, UEPB; A. B. SANTOS, UEPB; D. D. BARBOSA, UEPB; P. D. FERNANDES, UEPB; PAULO IVAN FERNANDES JUNIOR, CPATSA; LIZIANE MARIA DE LIMA, CNPA.BRITO, S. L.SANTOS, A. B.BARBOSA, D. D.FERNANDES, P. D.FERNANDES JUNIOR, P. I.LIMA, L. M. de2019-10-31T18:15:20Z2019-10-31T18:15:20Z2019-10-3120192019-10-31T18:15:20Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 18, n. 4, p. 1-12, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/111369910.4238/gmr18379enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-10-31T18:15:26Zoai:www.alice.cnptia.embrapa.br:doc/1113699Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-10-31T18:15:26falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-10-31T18:15:26Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
title |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
spellingShingle |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. BRITO, S. L. Fixação biológica de nitrogênio Genes ERF8 e NCED Tolerância à seca Arachis hipogaea Fixação de Nitrogênio Amendoim Leguminosa Genótipo Peanuts Bradyrhizobium Nitrogen fixation |
title_short |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
title_full |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
title_fullStr |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
title_full_unstemmed |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
title_sort |
Bradyrhizobium spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses. |
author |
BRITO, S. L. |
author_facet |
BRITO, S. L. SANTOS, A. B. BARBOSA, D. D. FERNANDES, P. D. FERNANDES JUNIOR, P. I. LIMA, L. M. de |
author_role |
author |
author2 |
SANTOS, A. B. BARBOSA, D. D. FERNANDES, P. D. FERNANDES JUNIOR, P. I. LIMA, L. M. de |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
S. L. BRITO, UEPB; A. B. SANTOS, UEPB; D. D. BARBOSA, UEPB; P. D. FERNANDES, UEPB; PAULO IVAN FERNANDES JUNIOR, CPATSA; LIZIANE MARIA DE LIMA, CNPA. |
dc.contributor.author.fl_str_mv |
BRITO, S. L. SANTOS, A. B. BARBOSA, D. D. FERNANDES, P. D. FERNANDES JUNIOR, P. I. LIMA, L. M. de |
dc.subject.por.fl_str_mv |
Fixação biológica de nitrogênio Genes ERF8 e NCED Tolerância à seca Arachis hipogaea Fixação de Nitrogênio Amendoim Leguminosa Genótipo Peanuts Bradyrhizobium Nitrogen fixation |
topic |
Fixação biológica de nitrogênio Genes ERF8 e NCED Tolerância à seca Arachis hipogaea Fixação de Nitrogênio Amendoim Leguminosa Genótipo Peanuts Bradyrhizobium Nitrogen fixation |
description |
The peanut plant has high plasticity and great adaptability to adverse conditions, including drought. To mitigate the negative effects of drought on legumes, nitrogen-fixing microorganisms have been investigated in some plant species, such as soybeans and beans. We analyzed the role Bradyrhizobium strains in peanut genotypes subjected to water deficit by means of plant growth, physiological and gene expression analysis. The research was conducted under greenhouse conditions with the runner peanut genotypes (IAC Runner 886, 2012-33 and 2012-47) and two Bradyrhizobium strains (ESA 123 and SEMIA 6144). After 20 days of germination, the water supply was completely interrupted and gas exchange analysis were carried out using an infrared gas analyzer, up to the 10th day of stress. Leaves were collected for the analysis of proline content and the expression of NCED and ERF8 genes. Analyses of plant height (PH), shoot dry mass (SDM), root dry mass (RDM), number of nodules (NN) and nodule weight (NW) also were performed. The peanut shoots inoculated with the Bradyrhizobium strains obtained the best results. The genotypes inoculated with the ESA 123 strain obtained superior responses compared to the nonstressed treatment inoculated with the same bacteria and the stressed control without bacterial inoculation. In the ESA 123 inoculated treatments, the water stressed plants had higher RDM (28.5% higher, on average), NN (two fold higher), and gene expression (approximately six and three-fold higher for ERF8 and NCED genes, respectively). The increase in the expression of NCED and ERF8 genes, in the three genotypes inoculated with ESA 123, suggests a key role of this inoculant in the activation of metabolic cascades for plant protection under water deficit. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-31T18:15:20Z 2019-10-31T18:15:20Z 2019-10-31 2019 2019-10-31T18:15:20Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 18, n. 4, p. 1-12, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113699 10.4238/gmr18379 |
identifier_str_mv |
Genetics and Molecular Research, v. 18, n. 4, p. 1-12, 2019. 10.4238/gmr18379 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113699 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503482829438976 |