Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085782 |
Resumo: | This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample. |
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Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.FeijãoPhaseolus vulgarisMarcador molecularMelhoramento genético vegetalThis manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.SAMARA RAYANE PEREIRA DE MORAIS, UFG; ARIADNA FARIA VIEIRA, UFMG; LAURA CRISTINA DA SILVA ALMEIDA, ufg; LUANA ALVES RODRIGUES, CNPAF; PATRICIA GUIMARAES SANTOS MELO, UFG; LUIS CLAUDIO DE FARIA, CNPAF; LEONARDO CUNHA MELO, CNPAF; HELTON SANTOS PEREIRA, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF.MORAIS, S. R. P. deVIEIRA, A. F.ALMEIDA, L. C. da S.RODRIGUES, L. A.MELO, P. G. S.FARIA, L. C. deMELO, L. C.PEREIRA, H. S.SOUZA, T. L. P. O. de2018-01-18T22:18:57Z2018-01-18T22:18:57Z2018-01-1820162018-01-18T22:18:57Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, p. 234-239, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/108578210.1590/1984-70332016v16n3n35enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-01-18T22:19:04Zoai:www.alice.cnptia.embrapa.br:doc/1085782Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-01-18T22:19:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-01-18T22:19:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
title |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
spellingShingle |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. MORAIS, S. R. P. de Feijão Phaseolus vulgaris Marcador molecular Melhoramento genético vegetal |
title_short |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
title_full |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
title_fullStr |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
title_full_unstemmed |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
title_sort |
Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean. |
author |
MORAIS, S. R. P. de |
author_facet |
MORAIS, S. R. P. de VIEIRA, A. F. ALMEIDA, L. C. da S. RODRIGUES, L. A. MELO, P. G. S. FARIA, L. C. de MELO, L. C. PEREIRA, H. S. SOUZA, T. L. P. O. de |
author_role |
author |
author2 |
VIEIRA, A. F. ALMEIDA, L. C. da S. RODRIGUES, L. A. MELO, P. G. S. FARIA, L. C. de MELO, L. C. PEREIRA, H. S. SOUZA, T. L. P. O. de |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
SAMARA RAYANE PEREIRA DE MORAIS, UFG; ARIADNA FARIA VIEIRA, UFMG; LAURA CRISTINA DA SILVA ALMEIDA, ufg; LUANA ALVES RODRIGUES, CNPAF; PATRICIA GUIMARAES SANTOS MELO, UFG; LUIS CLAUDIO DE FARIA, CNPAF; LEONARDO CUNHA MELO, CNPAF; HELTON SANTOS PEREIRA, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF. |
dc.contributor.author.fl_str_mv |
MORAIS, S. R. P. de VIEIRA, A. F. ALMEIDA, L. C. da S. RODRIGUES, L. A. MELO, P. G. S. FARIA, L. C. de MELO, L. C. PEREIRA, H. S. SOUZA, T. L. P. O. de |
dc.subject.por.fl_str_mv |
Feijão Phaseolus vulgaris Marcador molecular Melhoramento genético vegetal |
topic |
Feijão Phaseolus vulgaris Marcador molecular Melhoramento genético vegetal |
description |
This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2018-01-18T22:18:57Z 2018-01-18T22:18:57Z 2018-01-18 2018-01-18T22:18:57Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, p. 234-239, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085782 10.1590/1984-70332016v16n3n35 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, p. 234-239, 2016. 10.1590/1984-70332016v16n3n35 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085782 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503448259985408 |