Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.

Detalhes bibliográficos
Autor(a) principal: MORAIS, S. R. P. de
Data de Publicação: 2016
Outros Autores: VIEIRA, A. F., ALMEIDA, L. C. da S., RODRIGUES, L. A., MELO, P. G. S., FARIA, L. C. de, MELO, L. C., PEREIRA, H. S., SOUZA, T. L. P. O. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085782
Resumo: This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.
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spelling Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.FeijãoPhaseolus vulgarisMarcador molecularMelhoramento genético vegetalThis manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.SAMARA RAYANE PEREIRA DE MORAIS, UFG; ARIADNA FARIA VIEIRA, UFMG; LAURA CRISTINA DA SILVA ALMEIDA, ufg; LUANA ALVES RODRIGUES, CNPAF; PATRICIA GUIMARAES SANTOS MELO, UFG; LUIS CLAUDIO DE FARIA, CNPAF; LEONARDO CUNHA MELO, CNPAF; HELTON SANTOS PEREIRA, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF.MORAIS, S. R. P. deVIEIRA, A. F.ALMEIDA, L. C. da S.RODRIGUES, L. A.MELO, P. G. S.FARIA, L. C. deMELO, L. C.PEREIRA, H. S.SOUZA, T. L. P. O. de2018-01-18T22:18:57Z2018-01-18T22:18:57Z2018-01-1820162018-01-18T22:18:57Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, p. 234-239, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/108578210.1590/1984-70332016v16n3n35enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-01-18T22:19:04Zoai:www.alice.cnptia.embrapa.br:doc/1085782Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-01-18T22:19:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-01-18T22:19:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
title Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
spellingShingle Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
MORAIS, S. R. P. de
Feijão
Phaseolus vulgaris
Marcador molecular
Melhoramento genético vegetal
title_short Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
title_full Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
title_fullStr Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
title_full_unstemmed Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
title_sort Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean.
author MORAIS, S. R. P. de
author_facet MORAIS, S. R. P. de
VIEIRA, A. F.
ALMEIDA, L. C. da S.
RODRIGUES, L. A.
MELO, P. G. S.
FARIA, L. C. de
MELO, L. C.
PEREIRA, H. S.
SOUZA, T. L. P. O. de
author_role author
author2 VIEIRA, A. F.
ALMEIDA, L. C. da S.
RODRIGUES, L. A.
MELO, P. G. S.
FARIA, L. C. de
MELO, L. C.
PEREIRA, H. S.
SOUZA, T. L. P. O. de
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv SAMARA RAYANE PEREIRA DE MORAIS, UFG; ARIADNA FARIA VIEIRA, UFMG; LAURA CRISTINA DA SILVA ALMEIDA, ufg; LUANA ALVES RODRIGUES, CNPAF; PATRICIA GUIMARAES SANTOS MELO, UFG; LUIS CLAUDIO DE FARIA, CNPAF; LEONARDO CUNHA MELO, CNPAF; HELTON SANTOS PEREIRA, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF.
dc.contributor.author.fl_str_mv MORAIS, S. R. P. de
VIEIRA, A. F.
ALMEIDA, L. C. da S.
RODRIGUES, L. A.
MELO, P. G. S.
FARIA, L. C. de
MELO, L. C.
PEREIRA, H. S.
SOUZA, T. L. P. O. de
dc.subject.por.fl_str_mv Feijão
Phaseolus vulgaris
Marcador molecular
Melhoramento genético vegetal
topic Feijão
Phaseolus vulgaris
Marcador molecular
Melhoramento genético vegetal
description This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.
publishDate 2016
dc.date.none.fl_str_mv 2016
2018-01-18T22:18:57Z
2018-01-18T22:18:57Z
2018-01-18
2018-01-18T22:18:57Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, p. 234-239, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085782
10.1590/1984-70332016v16n3n35
identifier_str_mv Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 16, p. 234-239, 2016.
10.1590/1984-70332016v16n3n35
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085782
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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