1D mapping of seminal plasma proteins in Anglo-Nubian goats.

Detalhes bibliográficos
Autor(a) principal: TEIXEIRA, A. V. C.
Data de Publicação: 2009
Outros Autores: ELOY, A. M. X., FURTADO, J. R., PINHEIRO, R. R., PONTES, M. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/853159
Resumo: The lack of information about seasonal variation of seminal plasma proteins in goats from Northeast Brazil led us to investigate the protein band distribution throughout the year. The experiment lasted one year (from April 2006 to March 2007) in which 230 semen collections from five adult goats (Capra hircus) were carried out, at ages ranging from 18 to 22 months, weighing 42.0 ± 10.0 kg, belonging to Anglo Nubian breed. A pool of seminal plasma samples from all the animals in each month of the year was submitted to 1D electrophoresis SDS-PAGE, 12.5%. The gels were analyzed through Life Science Software from UVP, Inc®. The correlation between air humidity and total seminal plasma proteins was not significant (r = -0.24; P > 0.05), while a moderate and significant correlation was found between temperature and total protein (r = 0.39; P < 0.001). The semen volume and concentration showed statistical difference (P < 0.05) between months throughout the year, while the motility and vigor did not (P > 0.05). The analyzed gels showed a high frequency of molecular mass bands below 50 kDa throughout the year, followed by protein bands from 50 to 100 kDa. The major numbers of bands occurred in October and November, while May was the month that presented a smaller number of protein bands. The molecular bands of 14, 22, 24, 40, 55 and 66 kDa were present in all the months of the year. The band of 16 kDa was found in 41.7% of the months. May was the only month that did not show the 30 kDa. August and September did not present the bands of 34 and 35 kDa, while October showed both bands. The 45 kDa band was found in 75% of the months, being present in January, May, July, September and November. The 46 kDa band was identified in June and August, while the 47 kDa was identified in April. The 70 kDa band was identified from May to November (58.3%), the 72 kDa band was found in January, March and April (25%) and bands of 92 to 97 kDa were found from April to December (75%). These results showed a band distribution variation throughout the year in the Anglo Nubian goat breed in the semi-arid area of Brazil. It is concluded that medium rainfall or water scarcity in the dryer months seems to promote variation in semen volume and concentration and total protein distribution throughout the year. As no month variation was observed in semen motility and vigor, and protein bands probably related to fertility showed an uniform distribution throughout the year, it is suggested that there is no fertility variation during the year in Anglo-Nubian goats the semi-arid region.
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spelling 1D mapping of seminal plasma proteins in Anglo-Nubian goats.Plasma seminalRaça Anglo-NubianaCaprinoSêmenEletroforeseVariação sazonalGoatsElectrophoresisSeasonal variationThe lack of information about seasonal variation of seminal plasma proteins in goats from Northeast Brazil led us to investigate the protein band distribution throughout the year. The experiment lasted one year (from April 2006 to March 2007) in which 230 semen collections from five adult goats (Capra hircus) were carried out, at ages ranging from 18 to 22 months, weighing 42.0 ± 10.0 kg, belonging to Anglo Nubian breed. A pool of seminal plasma samples from all the animals in each month of the year was submitted to 1D electrophoresis SDS-PAGE, 12.5%. The gels were analyzed through Life Science Software from UVP, Inc®. The correlation between air humidity and total seminal plasma proteins was not significant (r = -0.24; P > 0.05), while a moderate and significant correlation was found between temperature and total protein (r = 0.39; P < 0.001). The semen volume and concentration showed statistical difference (P < 0.05) between months throughout the year, while the motility and vigor did not (P > 0.05). The analyzed gels showed a high frequency of molecular mass bands below 50 kDa throughout the year, followed by protein bands from 50 to 100 kDa. The major numbers of bands occurred in October and November, while May was the month that presented a smaller number of protein bands. The molecular bands of 14, 22, 24, 40, 55 and 66 kDa were present in all the months of the year. The band of 16 kDa was found in 41.7% of the months. May was the only month that did not show the 30 kDa. August and September did not present the bands of 34 and 35 kDa, while October showed both bands. The 45 kDa band was found in 75% of the months, being present in January, May, July, September and November. The 46 kDa band was identified in June and August, while the 47 kDa was identified in April. The 70 kDa band was identified from May to November (58.3%), the 72 kDa band was found in January, March and April (25%) and bands of 92 to 97 kDa were found from April to December (75%). These results showed a band distribution variation throughout the year in the Anglo Nubian goat breed in the semi-arid area of Brazil. It is concluded that medium rainfall or water scarcity in the dryer months seems to promote variation in semen volume and concentration and total protein distribution throughout the year. As no month variation was observed in semen motility and vigor, and protein bands probably related to fertility showed an uniform distribution throughout the year, it is suggested that there is no fertility variation during the year in Anglo-Nubian goats the semi-arid region.Meta 2010.Universidade Estadual Vale do Acaraú (UVA), Sobral, CE.; ANGELA MARIA XAVIER ELOY, CNPC; JOAO RICARDO FURTADO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; UVA.TEIXEIRA, A. V. C.ELOY, A. M. X.FURTADO, J. R.PINHEIRO, R. R.PONTES, M. S.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2010-05-1720092019-09-24T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimal Reproduction, v. 6, n. 4, p. 516-525, Oct./Dec. 2009.http://www.alice.cnptia.embrapa.br/alice/handle/doc/853159enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:35:44Zoai:www.alice.cnptia.embrapa.br:doc/853159Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:35:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:35:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
title 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
spellingShingle 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
TEIXEIRA, A. V. C.
Plasma seminal
Raça Anglo-Nubiana
Caprino
Sêmen
Eletroforese
Variação sazonal
Goats
Electrophoresis
Seasonal variation
title_short 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
title_full 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
title_fullStr 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
title_full_unstemmed 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
title_sort 1D mapping of seminal plasma proteins in Anglo-Nubian goats.
author TEIXEIRA, A. V. C.
author_facet TEIXEIRA, A. V. C.
ELOY, A. M. X.
FURTADO, J. R.
PINHEIRO, R. R.
PONTES, M. S.
author_role author
author2 ELOY, A. M. X.
FURTADO, J. R.
PINHEIRO, R. R.
PONTES, M. S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Vale do Acaraú (UVA), Sobral, CE.; ANGELA MARIA XAVIER ELOY, CNPC; JOAO RICARDO FURTADO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; UVA.
dc.contributor.author.fl_str_mv TEIXEIRA, A. V. C.
ELOY, A. M. X.
FURTADO, J. R.
PINHEIRO, R. R.
PONTES, M. S.
dc.subject.por.fl_str_mv Plasma seminal
Raça Anglo-Nubiana
Caprino
Sêmen
Eletroforese
Variação sazonal
Goats
Electrophoresis
Seasonal variation
topic Plasma seminal
Raça Anglo-Nubiana
Caprino
Sêmen
Eletroforese
Variação sazonal
Goats
Electrophoresis
Seasonal variation
description The lack of information about seasonal variation of seminal plasma proteins in goats from Northeast Brazil led us to investigate the protein band distribution throughout the year. The experiment lasted one year (from April 2006 to March 2007) in which 230 semen collections from five adult goats (Capra hircus) were carried out, at ages ranging from 18 to 22 months, weighing 42.0 ± 10.0 kg, belonging to Anglo Nubian breed. A pool of seminal plasma samples from all the animals in each month of the year was submitted to 1D electrophoresis SDS-PAGE, 12.5%. The gels were analyzed through Life Science Software from UVP, Inc®. The correlation between air humidity and total seminal plasma proteins was not significant (r = -0.24; P > 0.05), while a moderate and significant correlation was found between temperature and total protein (r = 0.39; P < 0.001). The semen volume and concentration showed statistical difference (P < 0.05) between months throughout the year, while the motility and vigor did not (P > 0.05). The analyzed gels showed a high frequency of molecular mass bands below 50 kDa throughout the year, followed by protein bands from 50 to 100 kDa. The major numbers of bands occurred in October and November, while May was the month that presented a smaller number of protein bands. The molecular bands of 14, 22, 24, 40, 55 and 66 kDa were present in all the months of the year. The band of 16 kDa was found in 41.7% of the months. May was the only month that did not show the 30 kDa. August and September did not present the bands of 34 and 35 kDa, while October showed both bands. The 45 kDa band was found in 75% of the months, being present in January, May, July, September and November. The 46 kDa band was identified in June and August, while the 47 kDa was identified in April. The 70 kDa band was identified from May to November (58.3%), the 72 kDa band was found in January, March and April (25%) and bands of 92 to 97 kDa were found from April to December (75%). These results showed a band distribution variation throughout the year in the Anglo Nubian goat breed in the semi-arid area of Brazil. It is concluded that medium rainfall or water scarcity in the dryer months seems to promote variation in semen volume and concentration and total protein distribution throughout the year. As no month variation was observed in semen motility and vigor, and protein bands probably related to fertility showed an uniform distribution throughout the year, it is suggested that there is no fertility variation during the year in Anglo-Nubian goats the semi-arid region.
publishDate 2009
dc.date.none.fl_str_mv 2009
2010-05-17
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2019-09-24T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Animal Reproduction, v. 6, n. 4, p. 516-525, Oct./Dec. 2009.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/853159
identifier_str_mv Animal Reproduction, v. 6, n. 4, p. 516-525, Oct./Dec. 2009.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/853159
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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