Comparison of protocols for chromosome duplication in haploid maize.

Detalhes bibliográficos
Autor(a) principal: AZEVEDO, T. C.
Data de Publicação: 2023
Outros Autores: GUIMARAES, F. F. M., TRINDADE, R. dos S., LANA, U. G. de P., SOUZA, I. R. P. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155039
https://doi.org/10.18512/rbms2023v22e1311
Resumo: Different methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks.
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spelling Comparison of protocols for chromosome duplication in haploid maize.Duplo haploideProduction of linesZea MaysMilhoLinhagemDoubled haploidsDifferent methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks.TÁCILA CRISTINA AZEVEDO, Universidade Federal de São João del-Rei; FLÁVIA FERREIRA MENDES GUIMARAES, Faculdades Ciências da Vida; ROBERTO DOS SANTOS TRINDADE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS.AZEVEDO, T. C.GUIMARAES, F. F. M.TRINDADE, R. dos S.LANA, U. G. de P.SOUZA, I. R. P. de2023-07-19T12:24:06Z2023-07-19T12:24:06Z2023-07-192023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Milho e Sorgo, v. 22, e1311, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155039https://doi.org/10.18512/rbms2023v22e1311enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-07-19T12:24:06Zoai:www.alice.cnptia.embrapa.br:doc/1155039Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-07-19T12:24:06falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-07-19T12:24:06Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Comparison of protocols for chromosome duplication in haploid maize.
title Comparison of protocols for chromosome duplication in haploid maize.
spellingShingle Comparison of protocols for chromosome duplication in haploid maize.
AZEVEDO, T. C.
Duplo haploide
Production of lines
Zea Mays
Milho
Linhagem
Doubled haploids
title_short Comparison of protocols for chromosome duplication in haploid maize.
title_full Comparison of protocols for chromosome duplication in haploid maize.
title_fullStr Comparison of protocols for chromosome duplication in haploid maize.
title_full_unstemmed Comparison of protocols for chromosome duplication in haploid maize.
title_sort Comparison of protocols for chromosome duplication in haploid maize.
author AZEVEDO, T. C.
author_facet AZEVEDO, T. C.
GUIMARAES, F. F. M.
TRINDADE, R. dos S.
LANA, U. G. de P.
SOUZA, I. R. P. de
author_role author
author2 GUIMARAES, F. F. M.
TRINDADE, R. dos S.
LANA, U. G. de P.
SOUZA, I. R. P. de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv TÁCILA CRISTINA AZEVEDO, Universidade Federal de São João del-Rei; FLÁVIA FERREIRA MENDES GUIMARAES, Faculdades Ciências da Vida; ROBERTO DOS SANTOS TRINDADE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS.
dc.contributor.author.fl_str_mv AZEVEDO, T. C.
GUIMARAES, F. F. M.
TRINDADE, R. dos S.
LANA, U. G. de P.
SOUZA, I. R. P. de
dc.subject.por.fl_str_mv Duplo haploide
Production of lines
Zea Mays
Milho
Linhagem
Doubled haploids
topic Duplo haploide
Production of lines
Zea Mays
Milho
Linhagem
Doubled haploids
description Different methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-19T12:24:06Z
2023-07-19T12:24:06Z
2023-07-19
2023
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Milho e Sorgo, v. 22, e1311, 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155039
https://doi.org/10.18512/rbms2023v22e1311
identifier_str_mv Revista Brasileira de Milho e Sorgo, v. 22, e1311, 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155039
https://doi.org/10.18512/rbms2023v22e1311
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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