Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.

Detalhes bibliográficos
Autor(a) principal: ROMÃO, N. F.
Data de Publicação: 2020
Outros Autores: PEREIRA, N. A. C., FUNES-HUACCA, M. E., BRITO, L. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123320
Resumo: Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.
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spelling Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.Portable DeviceBactériaBacterial contaminationEnvironmentPortable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.Natália Faria Romão, BIONORTE / UNIR; Nathalia Aparecida Costa Pereira, UNIR; Maribel Elizabeth Funes-Huacca, UNIR; LUCIANA GATTO BRITO, CPATU.ROMÃO, N. F.PEREIRA, N. A. C.FUNES-HUACCA, M. E.BRITO, L. G.2020-06-18T04:12:21Z2020-06-18T04:12:21Z2020-06-172020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAdvances in Microbiology, v. 10, n. 5, May 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112332010.4236/aim.2020.10518enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-06-18T04:12:31Zoai:www.alice.cnptia.embrapa.br:doc/1123320Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-06-18T04:12:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-06-18T04:12:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
title Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
spellingShingle Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
ROMÃO, N. F.
Portable Device
Bactéria
Bacterial contamination
Environment
title_short Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
title_full Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
title_fullStr Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
title_full_unstemmed Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
title_sort Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
author ROMÃO, N. F.
author_facet ROMÃO, N. F.
PEREIRA, N. A. C.
FUNES-HUACCA, M. E.
BRITO, L. G.
author_role author
author2 PEREIRA, N. A. C.
FUNES-HUACCA, M. E.
BRITO, L. G.
author2_role author
author
author
dc.contributor.none.fl_str_mv Natália Faria Romão, BIONORTE / UNIR; Nathalia Aparecida Costa Pereira, UNIR; Maribel Elizabeth Funes-Huacca, UNIR; LUCIANA GATTO BRITO, CPATU.
dc.contributor.author.fl_str_mv ROMÃO, N. F.
PEREIRA, N. A. C.
FUNES-HUACCA, M. E.
BRITO, L. G.
dc.subject.por.fl_str_mv Portable Device
Bactéria
Bacterial contamination
Environment
topic Portable Device
Bactéria
Bacterial contamination
Environment
description Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-18T04:12:21Z
2020-06-18T04:12:21Z
2020-06-17
2020
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Advances in Microbiology, v. 10, n. 5, May 2020.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123320
10.4236/aim.2020.10518
identifier_str_mv Advances in Microbiology, v. 10, n. 5, May 2020.
10.4236/aim.2020.10518
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123320
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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