Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123320 |
Resumo: | Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces. |
id |
EMBR_ba93f0e8166f9c44434509c6e7d91257 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1123320 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli.Portable DeviceBactériaBacterial contaminationEnvironmentPortable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.Natália Faria Romão, BIONORTE / UNIR; Nathalia Aparecida Costa Pereira, UNIR; Maribel Elizabeth Funes-Huacca, UNIR; LUCIANA GATTO BRITO, CPATU.ROMÃO, N. F.PEREIRA, N. A. C.FUNES-HUACCA, M. E.BRITO, L. G.2020-06-18T04:12:21Z2020-06-18T04:12:21Z2020-06-172020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAdvances in Microbiology, v. 10, n. 5, May 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112332010.4236/aim.2020.10518enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-06-18T04:12:31Zoai:www.alice.cnptia.embrapa.br:doc/1123320Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-06-18T04:12:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-06-18T04:12:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
title |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
spellingShingle |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. ROMÃO, N. F. Portable Device Bactéria Bacterial contamination Environment |
title_short |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
title_full |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
title_fullStr |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
title_full_unstemmed |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
title_sort |
Portable diagnostic platform for detection of microorganisms Coliforms and E. coli. |
author |
ROMÃO, N. F. |
author_facet |
ROMÃO, N. F. PEREIRA, N. A. C. FUNES-HUACCA, M. E. BRITO, L. G. |
author_role |
author |
author2 |
PEREIRA, N. A. C. FUNES-HUACCA, M. E. BRITO, L. G. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Natália Faria Romão, BIONORTE / UNIR; Nathalia Aparecida Costa Pereira, UNIR; Maribel Elizabeth Funes-Huacca, UNIR; LUCIANA GATTO BRITO, CPATU. |
dc.contributor.author.fl_str_mv |
ROMÃO, N. F. PEREIRA, N. A. C. FUNES-HUACCA, M. E. BRITO, L. G. |
dc.subject.por.fl_str_mv |
Portable Device Bactéria Bacterial contamination Environment |
topic |
Portable Device Bactéria Bacterial contamination Environment |
description |
Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-18T04:12:21Z 2020-06-18T04:12:21Z 2020-06-17 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Advances in Microbiology, v. 10, n. 5, May 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123320 10.4236/aim.2020.10518 |
identifier_str_mv |
Advances in Microbiology, v. 10, n. 5, May 2020. 10.4236/aim.2020.10518 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123320 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503493211389952 |