Exploratory
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081992 |
Resumo: | Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling. |
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ExploratoryCoffeeSlow coolingLiquid nitrogenTeor de águaResfriamento lentoNitrogênio líquidoCaféWater contentsilica gelCryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.Título em português: Estudos exploratórios para a criopreservação de sementes de Coffea arabica L.MADELEINE ALVES DE FIGUEIREDO, DA/UFLA; STEFANIA VILAS BOAS COELHO, DA/UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; AMANDA LIMA VILELA, DA/UFLA; LUCIANO COUTINHO SILVA, DBCM/UFPB.FIGUEIREDO, M. A. deCOELHO, S. V. B.ROSA, S. D. V. F. daVILELA, A. L.SILVA, L. C.2017-12-08T23:27:35Z2017-12-08T23:27:35Z2017-12-0820172017-12-08T23:27:35Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Seed Science, v. 39, n. 2, p. 150-158, 2017http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081992enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-12-08T23:27:42Zoai:www.alice.cnptia.embrapa.br:doc/1081992Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-12-08T23:27:42falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-12-08T23:27:42Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Exploratory |
title |
Exploratory |
spellingShingle |
Exploratory FIGUEIREDO, M. A. de Coffee Slow cooling Liquid nitrogen Teor de água Resfriamento lento Nitrogênio líquido Café Water content silica gel |
title_short |
Exploratory |
title_full |
Exploratory |
title_fullStr |
Exploratory |
title_full_unstemmed |
Exploratory |
title_sort |
Exploratory |
author |
FIGUEIREDO, M. A. de |
author_facet |
FIGUEIREDO, M. A. de COELHO, S. V. B. ROSA, S. D. V. F. da VILELA, A. L. SILVA, L. C. |
author_role |
author |
author2 |
COELHO, S. V. B. ROSA, S. D. V. F. da VILELA, A. L. SILVA, L. C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
MADELEINE ALVES DE FIGUEIREDO, DA/UFLA; STEFANIA VILAS BOAS COELHO, DA/UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; AMANDA LIMA VILELA, DA/UFLA; LUCIANO COUTINHO SILVA, DBCM/UFPB. |
dc.contributor.author.fl_str_mv |
FIGUEIREDO, M. A. de COELHO, S. V. B. ROSA, S. D. V. F. da VILELA, A. L. SILVA, L. C. |
dc.subject.por.fl_str_mv |
Coffee Slow cooling Liquid nitrogen Teor de água Resfriamento lento Nitrogênio líquido Café Water content silica gel |
topic |
Coffee Slow cooling Liquid nitrogen Teor de água Resfriamento lento Nitrogênio líquido Café Water content silica gel |
description |
Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-08T23:27:35Z 2017-12-08T23:27:35Z 2017-12-08 2017 2017-12-08T23:27:35Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Seed Science, v. 39, n. 2, p. 150-158, 2017 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081992 |
identifier_str_mv |
Journal of Seed Science, v. 39, n. 2, p. 150-158, 2017 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503446110404608 |