Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156508 https://doi.org/10.1016/j.bej.2023.109022 |
Resumo: | Abstract: Agro-industrial waste byproducts are commonly inexpensive organic nutritional sources for production of filamentous fungi, and selection of an optimal bioreactor is a key factor for attaining satisfactory biomass yield and quality. The insect-pathogenic fungus Beauveria bassiana (Hypocreales: Cordycipitaceae) has been extensively mass produced by solid-state fermentation using precooked cereal grains. Here, we propose low-cost palm oil residues as the main substrate using a cylindrical packed-column aerobic bioreactor prototype designed for conidial production of B. bassiana. Fermentation treatments using B. bassiana strain CG1229 assessed the impact of temperature, air flow, substrate moisture content, and ratio between palm fiber (PF) and palm kernel cake (PKC) on conidial yield and desiccation tolerance. Results showed significant enhanced productivity (2 ×1010 conidia g?1 dry matter) and reduced fermentation time (from 168 h to 120 h) by this method compared with the conventional tray bioreactor. Optimal substrate conditions were 60% initial moisture and 30% PF + 70% PKC content, fermentation at 26 °C with aeration rate ? 0.2 L min?1. Air drying conidia inside the column yielded > 95% germination. Maximum spore production was achieved in 120 h. Our findings demonstrate the feasibility of using a packed-column bioreactor for mass production of B. bassiana conidia using low-cost agricultural residues, which contributes to a sustainable production method of this biopesticide in Brazil. |
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Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues.Forced aerationWaste valorizationBeauveria BassianaFungo Para Controle BiológicoProduçãoFermentaçãoResíduo OrgânicoElaeis GuineensisBiological control agentsEntomopathogenic fungiSolid state fermentationPlant residuesPalm oilsbiopesticidesAbstract: Agro-industrial waste byproducts are commonly inexpensive organic nutritional sources for production of filamentous fungi, and selection of an optimal bioreactor is a key factor for attaining satisfactory biomass yield and quality. The insect-pathogenic fungus Beauveria bassiana (Hypocreales: Cordycipitaceae) has been extensively mass produced by solid-state fermentation using precooked cereal grains. Here, we propose low-cost palm oil residues as the main substrate using a cylindrical packed-column aerobic bioreactor prototype designed for conidial production of B. bassiana. Fermentation treatments using B. bassiana strain CG1229 assessed the impact of temperature, air flow, substrate moisture content, and ratio between palm fiber (PF) and palm kernel cake (PKC) on conidial yield and desiccation tolerance. Results showed significant enhanced productivity (2 ×1010 conidia g?1 dry matter) and reduced fermentation time (from 168 h to 120 h) by this method compared with the conventional tray bioreactor. Optimal substrate conditions were 60% initial moisture and 30% PF + 70% PKC content, fermentation at 26 °C with aeration rate ? 0.2 L min?1. Air drying conidia inside the column yielded > 95% germination. Maximum spore production was achieved in 120 h. Our findings demonstrate the feasibility of using a packed-column bioreactor for mass production of B. bassiana conidia using low-cost agricultural residues, which contributes to a sustainable production method of this biopesticide in Brazil.JAQUELINE DO NASCIMENTO SILVA, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; GABRIEL MOURA MASCARIN, CNPMA; ROGERIO BIAGGIONI LOPES, Cenargen; DENISE MARIA GUIMARÃES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO.SILVA, J. do N.MASCARIN, G. M.LOPES, R. B.FREIRE, D. M. G.2023-09-08T11:23:47Z2023-09-08T11:23:47Z2023-09-082023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiochemical Engineering Journal, v. 198, article 109022, 2023.1369-703Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1156508https://doi.org/10.1016/j.bej.2023.109022enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-08T11:23:47Zoai:www.alice.cnptia.embrapa.br:doc/1156508Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-08T11:23:47falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-08T11:23:47Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
title |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
spellingShingle |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. SILVA, J. do N. Forced aeration Waste valorization Beauveria Bassiana Fungo Para Controle Biológico Produção Fermentação Resíduo Orgânico Elaeis Guineensis Biological control agents Entomopathogenic fungi Solid state fermentation Plant residues Palm oils biopesticides |
title_short |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
title_full |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
title_fullStr |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
title_full_unstemmed |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
title_sort |
Production of dried Beauveria bassiana conidia in packed-column bioreactor using agro-industrial palm oil residues. |
author |
SILVA, J. do N. |
author_facet |
SILVA, J. do N. MASCARIN, G. M. LOPES, R. B. FREIRE, D. M. G. |
author_role |
author |
author2 |
MASCARIN, G. M. LOPES, R. B. FREIRE, D. M. G. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
JAQUELINE DO NASCIMENTO SILVA, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; GABRIEL MOURA MASCARIN, CNPMA; ROGERIO BIAGGIONI LOPES, Cenargen; DENISE MARIA GUIMARÃES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
dc.contributor.author.fl_str_mv |
SILVA, J. do N. MASCARIN, G. M. LOPES, R. B. FREIRE, D. M. G. |
dc.subject.por.fl_str_mv |
Forced aeration Waste valorization Beauveria Bassiana Fungo Para Controle Biológico Produção Fermentação Resíduo Orgânico Elaeis Guineensis Biological control agents Entomopathogenic fungi Solid state fermentation Plant residues Palm oils biopesticides |
topic |
Forced aeration Waste valorization Beauveria Bassiana Fungo Para Controle Biológico Produção Fermentação Resíduo Orgânico Elaeis Guineensis Biological control agents Entomopathogenic fungi Solid state fermentation Plant residues Palm oils biopesticides |
description |
Abstract: Agro-industrial waste byproducts are commonly inexpensive organic nutritional sources for production of filamentous fungi, and selection of an optimal bioreactor is a key factor for attaining satisfactory biomass yield and quality. The insect-pathogenic fungus Beauveria bassiana (Hypocreales: Cordycipitaceae) has been extensively mass produced by solid-state fermentation using precooked cereal grains. Here, we propose low-cost palm oil residues as the main substrate using a cylindrical packed-column aerobic bioreactor prototype designed for conidial production of B. bassiana. Fermentation treatments using B. bassiana strain CG1229 assessed the impact of temperature, air flow, substrate moisture content, and ratio between palm fiber (PF) and palm kernel cake (PKC) on conidial yield and desiccation tolerance. Results showed significant enhanced productivity (2 ×1010 conidia g?1 dry matter) and reduced fermentation time (from 168 h to 120 h) by this method compared with the conventional tray bioreactor. Optimal substrate conditions were 60% initial moisture and 30% PF + 70% PKC content, fermentation at 26 °C with aeration rate ? 0.2 L min?1. Air drying conidia inside the column yielded > 95% germination. Maximum spore production was achieved in 120 h. Our findings demonstrate the feasibility of using a packed-column bioreactor for mass production of B. bassiana conidia using low-cost agricultural residues, which contributes to a sustainable production method of this biopesticide in Brazil. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-08T11:23:47Z 2023-09-08T11:23:47Z 2023-09-08 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Biochemical Engineering Journal, v. 198, article 109022, 2023. 1369-703X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156508 https://doi.org/10.1016/j.bej.2023.109022 |
identifier_str_mv |
Biochemical Engineering Journal, v. 198, article 109022, 2023. 1369-703X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156508 https://doi.org/10.1016/j.bej.2023.109022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549128802304 |