Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.

Detalhes bibliográficos
Autor(a) principal: OLIVEIRA, L. C. de
Data de Publicação: 2023
Outros Autores: NAKASONE, A. K., SILVA, C. S., BONFIM, K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152411
https://doi.org/10.1590/0034-737X202370010014
Resumo: Bacterial spot, caused by Xanthomonas axonopodis pv. passiflorae, is a disease that has limited the cultivation of passionfruit in various orchards in Brazil. The objective of this work is to characterize and evaluate the variability of 29 strains of X. axonopodis pv. passiflorae from different municipalities producing yellow passionfruit in the state of Pará. The characterization was performed by the biochemical methods of KOH solubility, oxidase and Bactray, and the molecular methods of Xapas-F, Xapas-Ri and Xapas-Ro primers. The variability was evaluated by pathogenicity test and RAPD (randomly amplified polymorphic DNA). The strains of X. axonopodis pv. passiflorae were Gram-negative and oxidase-negative, and tests with the Bactray kit showed no relation between the collection municipality and group composition. The Xapas-F/Ri/Ro primers were specific for the strains. The primers used amplified 118 polymorphic bands in the RAPD reactions and the highest genetic similarity was between the strains PA15 and PA16. As the pathogenicity test evidenced a pathogenic variability, the strains PA2.1, PA4.5, PA14, PA4.2, PA4.1, PA4.6, PA3.4 and PA4.3 present the highest severity values for the disease. The strains of X. axonopodis pv. passiflorae show characteristics typical of the species, and genetic and pathogenic variability among them.
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spelling Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.Bacterial spotBiochemical testsMaracujáPassiflora Edulis FlavicarpaDoença de PlantaMancha BacterianaDetectionBacterial spot, caused by Xanthomonas axonopodis pv. passiflorae, is a disease that has limited the cultivation of passionfruit in various orchards in Brazil. The objective of this work is to characterize and evaluate the variability of 29 strains of X. axonopodis pv. passiflorae from different municipalities producing yellow passionfruit in the state of Pará. The characterization was performed by the biochemical methods of KOH solubility, oxidase and Bactray, and the molecular methods of Xapas-F, Xapas-Ri and Xapas-Ro primers. The variability was evaluated by pathogenicity test and RAPD (randomly amplified polymorphic DNA). The strains of X. axonopodis pv. passiflorae were Gram-negative and oxidase-negative, and tests with the Bactray kit showed no relation between the collection municipality and group composition. The Xapas-F/Ri/Ro primers were specific for the strains. The primers used amplified 118 polymorphic bands in the RAPD reactions and the highest genetic similarity was between the strains PA15 and PA16. As the pathogenicity test evidenced a pathogenic variability, the strains PA2.1, PA4.5, PA14, PA4.2, PA4.1, PA4.6, PA3.4 and PA4.3 present the highest severity values for the disease. The strains of X. axonopodis pv. passiflorae show characteristics typical of the species, and genetic and pathogenic variability among them.LUANA CARDOSO DE OLIVEIRA, UNIVERSIDADE FEDERAL DO PARÁ; ALESSANDRA KEIKO NAKASONE, CPATU; CAIO SANTOS SILVA, UNIVERSIDADE FEDERAL DO PARÁ; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen.OLIVEIRA, L. C. deNAKASONE, A. K.SILVA, C. S.BONFIM, K.2023-03-16T13:51:16Z2023-03-16T13:51:16Z2023-03-162023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Ceres, Viçosa, v. 70, n. 1, p. 124-132, jan./feb. 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152411https://doi.org/10.1590/0034-737X202370010014enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-03-16T13:51:16Zoai:www.alice.cnptia.embrapa.br:doc/1152411Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-03-16T13:51:16falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-03-16T13:51:16Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
title Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
spellingShingle Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
OLIVEIRA, L. C. de
Bacterial spot
Biochemical tests
Maracujá
Passiflora Edulis Flavicarpa
Doença de Planta
Mancha Bacteriana
Detection
title_short Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
title_full Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
title_fullStr Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
title_full_unstemmed Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
title_sort Characterization and variability of strains of Xanthomonas axonopodis pv. passiflorae from the state of Pará, Brazil.
author OLIVEIRA, L. C. de
author_facet OLIVEIRA, L. C. de
NAKASONE, A. K.
SILVA, C. S.
BONFIM, K.
author_role author
author2 NAKASONE, A. K.
SILVA, C. S.
BONFIM, K.
author2_role author
author
author
dc.contributor.none.fl_str_mv LUANA CARDOSO DE OLIVEIRA, UNIVERSIDADE FEDERAL DO PARÁ; ALESSANDRA KEIKO NAKASONE, CPATU; CAIO SANTOS SILVA, UNIVERSIDADE FEDERAL DO PARÁ; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen.
dc.contributor.author.fl_str_mv OLIVEIRA, L. C. de
NAKASONE, A. K.
SILVA, C. S.
BONFIM, K.
dc.subject.por.fl_str_mv Bacterial spot
Biochemical tests
Maracujá
Passiflora Edulis Flavicarpa
Doença de Planta
Mancha Bacteriana
Detection
topic Bacterial spot
Biochemical tests
Maracujá
Passiflora Edulis Flavicarpa
Doença de Planta
Mancha Bacteriana
Detection
description Bacterial spot, caused by Xanthomonas axonopodis pv. passiflorae, is a disease that has limited the cultivation of passionfruit in various orchards in Brazil. The objective of this work is to characterize and evaluate the variability of 29 strains of X. axonopodis pv. passiflorae from different municipalities producing yellow passionfruit in the state of Pará. The characterization was performed by the biochemical methods of KOH solubility, oxidase and Bactray, and the molecular methods of Xapas-F, Xapas-Ri and Xapas-Ro primers. The variability was evaluated by pathogenicity test and RAPD (randomly amplified polymorphic DNA). The strains of X. axonopodis pv. passiflorae were Gram-negative and oxidase-negative, and tests with the Bactray kit showed no relation between the collection municipality and group composition. The Xapas-F/Ri/Ro primers were specific for the strains. The primers used amplified 118 polymorphic bands in the RAPD reactions and the highest genetic similarity was between the strains PA15 and PA16. As the pathogenicity test evidenced a pathogenic variability, the strains PA2.1, PA4.5, PA14, PA4.2, PA4.1, PA4.6, PA3.4 and PA4.3 present the highest severity values for the disease. The strains of X. axonopodis pv. passiflorae show characteristics typical of the species, and genetic and pathogenic variability among them.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-16T13:51:16Z
2023-03-16T13:51:16Z
2023-03-16
2023
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Ceres, Viçosa, v. 70, n. 1, p. 124-132, jan./feb. 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152411
https://doi.org/10.1590/0034-737X202370010014
identifier_str_mv Revista Ceres, Viçosa, v. 70, n. 1, p. 124-132, jan./feb. 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152411
https://doi.org/10.1590/0034-737X202370010014
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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