Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598 |
Resumo: | The use of fluorescent-labelled simple sequence repeat (SSR) markers allows the simultaneous analysis of diverse loci in multiplex series, providing an enhanced precision of the determination of the allele size when a large number of genotypes is used. Twenty-five fluorescent-labelled SSR markers were used to genotype 242 accessions of the Brazilian Rice Core Collection. Based on the Polymorphism Information Content (PIC) values of each marker, groups with 5, 10, 15 and 20 markers were formed. The average PIC values varied from 0.67 to 0.89 and the number of alleles sampled per group, from 70 to 377. In the case of rice, which has a narrow genetic base, the conclusion was drawn that it is of fundamental importance that the genotypes must be evaluated by the highest possible number of regularly spaced SSR markers in the genome, and that these, preferentially, should offer a high information content. |
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Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization.Diversidade genéticaRecursos genéticosGenetic diversityArrozOryza Sativagenetic resourcesriceThe use of fluorescent-labelled simple sequence repeat (SSR) markers allows the simultaneous analysis of diverse loci in multiplex series, providing an enhanced precision of the determination of the allele size when a large number of genotypes is used. Twenty-five fluorescent-labelled SSR markers were used to genotype 242 accessions of the Brazilian Rice Core Collection. Based on the Polymorphism Information Content (PIC) values of each marker, groups with 5, 10, 15 and 20 markers were formed. The average PIC values varied from 0.67 to 0.89 and the number of alleles sampled per group, from 70 to 377. In the case of rice, which has a narrow genetic base, the conclusion was drawn that it is of fundamental importance that the genotypes must be evaluated by the highest possible number of regularly spaced SSR markers in the genome, and that these, preferentially, should offer a high information content.TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; CLAUDIO BRONDANI, CNPAF.BORBA, T. C. de O.BRONDANI, R. V.RANGEL, P. H. N.BRONDANI, C.2011-04-09T14:33:24Z2011-04-09T14:33:24Z2005-08-1920052011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, v. 5, n. 2, p. 157-165, June 2005.http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:34:07Zoai:www.alice.cnptia.embrapa.br:doc/213598Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:34:07falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:34:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
spellingShingle |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. BORBA, T. C. de O. Diversidade genética Recursos genéticos Genetic diversity Arroz Oryza Sativa genetic resources rice |
title_short |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_full |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_fullStr |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_full_unstemmed |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
title_sort |
Evaluation of the number and information content of fluorescent-labeled SSR markers for rice germplasm characterization. |
author |
BORBA, T. C. de O. |
author_facet |
BORBA, T. C. de O. BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. |
author_role |
author |
author2 |
BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; CLAUDIO BRONDANI, CNPAF. |
dc.contributor.author.fl_str_mv |
BORBA, T. C. de O. BRONDANI, R. V. RANGEL, P. H. N. BRONDANI, C. |
dc.subject.por.fl_str_mv |
Diversidade genética Recursos genéticos Genetic diversity Arroz Oryza Sativa genetic resources rice |
topic |
Diversidade genética Recursos genéticos Genetic diversity Arroz Oryza Sativa genetic resources rice |
description |
The use of fluorescent-labelled simple sequence repeat (SSR) markers allows the simultaneous analysis of diverse loci in multiplex series, providing an enhanced precision of the determination of the allele size when a large number of genotypes is used. Twenty-five fluorescent-labelled SSR markers were used to genotype 242 accessions of the Brazilian Rice Core Collection. Based on the Polymorphism Information Content (PIC) values of each marker, groups with 5, 10, 15 and 20 markers were formed. The average PIC values varied from 0.67 to 0.89 and the number of alleles sampled per group, from 70 to 377. In the case of rice, which has a narrow genetic base, the conclusion was drawn that it is of fundamental importance that the genotypes must be evaluated by the highest possible number of regularly spaced SSR markers in the genome, and that these, preferentially, should offer a high information content. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-08-19 2005 2011-04-09T14:33:24Z 2011-04-09T14:33:24Z 2011-04-10T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, v. 5, n. 2, p. 157-165, June 2005. http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, v. 5, n. 2, p. 157-165, June 2005. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/213598 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503320820252672 |