Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.

Detalhes bibliográficos
Autor(a) principal: AGUIAR, N. O.
Data de Publicação: 2018
Outros Autores: OLIVARES, F. L., NOVOTNY, E. H., CANELLAS, L. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100181
https://doi.org/10.7717/peerj.5445
Resumo: Plant growth-promoting bacteria (PGPB) and humic acids (HA) have been used as biostimulants in field conditions. The complete genomic and proteomic transcription of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus is available but interpreting and utilizing this information in the field to increase crop performance is challenging. The identification and characterization of metabolites that are induced by genomic changes may be used to improve plant responses to inoculation. The objective of this study was to describe changes in sugarcane metabolic profile that occur when HA and PGPB are used as biostimulants. Inoculum was applied to soil containing 45-day old sugarcane stalks. One week after inoculation, the methanolic extracts from leaves were obtained and analyzed by gas chromatography coupled to time-of-flight mass spectrometry; a total of 1,880 compounds were observed and 280 were identified in all samples. The application of HA significantly decreased the concentration of 15 metabolites, which generally included amino acids. HA increased the levels of 40 compounds, and these included metabolites linked to the stress response (shikimic, caffeic, hydroxycinnamic acids, putrescine, behenic acid, quinoline xylulose, galactose, lactose proline, oxyproline and valeric acid) and cellular growth (adenine and adenosine derivatives, ribose, ribonic acid and citric acid). Similarly, PGPB enhanced the level of metabolites identified in HA-treated soils; e.g., 48 metabolites were elevated and included amino acids, nucleic acids, organic acids, and lipids. Co-inoculation (HACPGPB) boosted the level of 110 metabolites with respect to non-inoculated controls; these included amino acids, lipids and nitrogenous compounds. Changes in the metabolic profile induced by HA+PGPB influenced both glucose and pentose pathways and resulted in the accumulation of heptuloses and riboses, which are substrates in the nucleoside biosynthesis and shikimic acid pathways. The mevalonate pathway was also activated, thus increasing phytosterol synthesis. The improvement in cellular metabolism observed with PGPB+HA was compatible with high levels of vitamins. Glucuronate and amino sugars were stimulated in addition to the products and intermediary compounds of tricarboxylic acid metabolism. Lipids and amino acids were the main compounds induced by co-inoculation in addition to antioxidants, stress-related metabolites, and compounds involved in cellular redox. The primary compounds observed in each treatment were identified, and the effect of co-inoculation (HACPGPB) on metabolite levels was discussed.
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spelling Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.Substância húmicaBactéria fixadora de nitrogênioBioestimulantePromoção do crescimento vegetalBiofertilizanteHumic substancesNitrogen-fixing bacteriaMetabolomicsPlant growthBiofertilizersPlant growth-promoting bacteria (PGPB) and humic acids (HA) have been used as biostimulants in field conditions. The complete genomic and proteomic transcription of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus is available but interpreting and utilizing this information in the field to increase crop performance is challenging. The identification and characterization of metabolites that are induced by genomic changes may be used to improve plant responses to inoculation. The objective of this study was to describe changes in sugarcane metabolic profile that occur when HA and PGPB are used as biostimulants. Inoculum was applied to soil containing 45-day old sugarcane stalks. One week after inoculation, the methanolic extracts from leaves were obtained and analyzed by gas chromatography coupled to time-of-flight mass spectrometry; a total of 1,880 compounds were observed and 280 were identified in all samples. The application of HA significantly decreased the concentration of 15 metabolites, which generally included amino acids. HA increased the levels of 40 compounds, and these included metabolites linked to the stress response (shikimic, caffeic, hydroxycinnamic acids, putrescine, behenic acid, quinoline xylulose, galactose, lactose proline, oxyproline and valeric acid) and cellular growth (adenine and adenosine derivatives, ribose, ribonic acid and citric acid). Similarly, PGPB enhanced the level of metabolites identified in HA-treated soils; e.g., 48 metabolites were elevated and included amino acids, nucleic acids, organic acids, and lipids. Co-inoculation (HACPGPB) boosted the level of 110 metabolites with respect to non-inoculated controls; these included amino acids, lipids and nitrogenous compounds. Changes in the metabolic profile induced by HA+PGPB influenced both glucose and pentose pathways and resulted in the accumulation of heptuloses and riboses, which are substrates in the nucleoside biosynthesis and shikimic acid pathways. The mevalonate pathway was also activated, thus increasing phytosterol synthesis. The improvement in cellular metabolism observed with PGPB+HA was compatible with high levels of vitamins. Glucuronate and amino sugars were stimulated in addition to the products and intermediary compounds of tricarboxylic acid metabolism. Lipids and amino acids were the main compounds induced by co-inoculation in addition to antioxidants, stress-related metabolites, and compounds involved in cellular redox. The primary compounds observed in each treatment were identified, and the effect of co-inoculation (HACPGPB) on metabolite levels was discussed.NATALIA O. AGUIAR, UENF; FABIO L. OLIVARES, UENF; ETELVINO HENRIQUE NOVOTNY, CNPS; LUCIANO P. CANELLAS, UENF.AGUIAR, N. O.OLIVARES, F. L.NOVOTNY, E. H.CANELLAS, L. P.2018-11-27T23:38:43Z2018-11-27T23:38:43Z2018-11-2720182018-11-27T23:38:43Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePeerJ, v. 6, article e5445, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100181https://doi.org/10.7717/peerj.5445enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-11-27T23:38:49Zoai:www.alice.cnptia.embrapa.br:doc/1100181Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-11-27T23:38:49falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-11-27T23:38:49Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
title Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
spellingShingle Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
AGUIAR, N. O.
Substância húmica
Bactéria fixadora de nitrogênio
Bioestimulante
Promoção do crescimento vegetal
Biofertilizante
Humic substances
Nitrogen-fixing bacteria
Metabolomics
Plant growth
Biofertilizers
title_short Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
title_full Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
title_fullStr Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
title_full_unstemmed Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
title_sort Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.
author AGUIAR, N. O.
author_facet AGUIAR, N. O.
OLIVARES, F. L.
NOVOTNY, E. H.
CANELLAS, L. P.
author_role author
author2 OLIVARES, F. L.
NOVOTNY, E. H.
CANELLAS, L. P.
author2_role author
author
author
dc.contributor.none.fl_str_mv NATALIA O. AGUIAR, UENF; FABIO L. OLIVARES, UENF; ETELVINO HENRIQUE NOVOTNY, CNPS; LUCIANO P. CANELLAS, UENF.
dc.contributor.author.fl_str_mv AGUIAR, N. O.
OLIVARES, F. L.
NOVOTNY, E. H.
CANELLAS, L. P.
dc.subject.por.fl_str_mv Substância húmica
Bactéria fixadora de nitrogênio
Bioestimulante
Promoção do crescimento vegetal
Biofertilizante
Humic substances
Nitrogen-fixing bacteria
Metabolomics
Plant growth
Biofertilizers
topic Substância húmica
Bactéria fixadora de nitrogênio
Bioestimulante
Promoção do crescimento vegetal
Biofertilizante
Humic substances
Nitrogen-fixing bacteria
Metabolomics
Plant growth
Biofertilizers
description Plant growth-promoting bacteria (PGPB) and humic acids (HA) have been used as biostimulants in field conditions. The complete genomic and proteomic transcription of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus is available but interpreting and utilizing this information in the field to increase crop performance is challenging. The identification and characterization of metabolites that are induced by genomic changes may be used to improve plant responses to inoculation. The objective of this study was to describe changes in sugarcane metabolic profile that occur when HA and PGPB are used as biostimulants. Inoculum was applied to soil containing 45-day old sugarcane stalks. One week after inoculation, the methanolic extracts from leaves were obtained and analyzed by gas chromatography coupled to time-of-flight mass spectrometry; a total of 1,880 compounds were observed and 280 were identified in all samples. The application of HA significantly decreased the concentration of 15 metabolites, which generally included amino acids. HA increased the levels of 40 compounds, and these included metabolites linked to the stress response (shikimic, caffeic, hydroxycinnamic acids, putrescine, behenic acid, quinoline xylulose, galactose, lactose proline, oxyproline and valeric acid) and cellular growth (adenine and adenosine derivatives, ribose, ribonic acid and citric acid). Similarly, PGPB enhanced the level of metabolites identified in HA-treated soils; e.g., 48 metabolites were elevated and included amino acids, nucleic acids, organic acids, and lipids. Co-inoculation (HACPGPB) boosted the level of 110 metabolites with respect to non-inoculated controls; these included amino acids, lipids and nitrogenous compounds. Changes in the metabolic profile induced by HA+PGPB influenced both glucose and pentose pathways and resulted in the accumulation of heptuloses and riboses, which are substrates in the nucleoside biosynthesis and shikimic acid pathways. The mevalonate pathway was also activated, thus increasing phytosterol synthesis. The improvement in cellular metabolism observed with PGPB+HA was compatible with high levels of vitamins. Glucuronate and amino sugars were stimulated in addition to the products and intermediary compounds of tricarboxylic acid metabolism. Lipids and amino acids were the main compounds induced by co-inoculation in addition to antioxidants, stress-related metabolites, and compounds involved in cellular redox. The primary compounds observed in each treatment were identified, and the effect of co-inoculation (HACPGPB) on metabolite levels was discussed.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-27T23:38:43Z
2018-11-27T23:38:43Z
2018-11-27
2018
2018-11-27T23:38:43Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PeerJ, v. 6, article e5445, 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100181
https://doi.org/10.7717/peerj.5445
identifier_str_mv PeerJ, v. 6, article e5445, 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100181
https://doi.org/10.7717/peerj.5445
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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