Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.

Detalhes bibliográficos
Autor(a) principal: PEREIRA, G. A.
Data de Publicação: 2020
Outros Autores: SANTOS, E. M., ARAUJO, G. G. L. de, OLIVEIRA, J. S., PINHO, R. M. A, ZANINE, A. de M., SOUZA, A. F. N., MACEDO, A. J. S., NETO, J. M. C., NASCIMENTO, T. V. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082
https:// doi.org/10.1017/S0021859620000143
Resumo: The current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage.
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spelling Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.Cepas de bactéria de ácido lácticoFermentação da silagemAlimentação animalSilagemPalma ForrageiraBactéria AeróbiaFermentação AeróbicaFermentação AcidaNutrição AnimalAlimentaçãoOpuntiaSilageSilage fermentationThe current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage.G. A. Pereira; E. M. Santos; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; J. S. Oliveira; R. M. A. Pinho; A. de M. Zanine; A. F. N. Souza; A. J. S. Macedo; J. M. C. Neto; T. V. C. Nascimento.PEREIRA, G. A.SANTOS, E. M.ARAUJO, G. G. L. deOLIVEIRA, J. S.PINHO, R. M. AZANINE, A. de M.SOUZA, A. F. N.MACEDO, A. J. S.NETO, J. M. C.NASCIMENTO, T. V. C.2021-02-18T15:18:10Z2021-02-18T15:18:10Z2021-02-182020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe Journal of Agricultural Science, v. 157, n. 9-10, p. 684-692, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082https:// doi.org/10.1017/S0021859620000143enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-02-18T15:18:20Zoai:www.alice.cnptia.embrapa.br:doc/1130082Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-02-18T15:18:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-02-18T15:18:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
title Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
spellingShingle Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
PEREIRA, G. A.
Cepas de bactéria de ácido láctico
Fermentação da silagem
Alimentação animal
Silagem
Palma Forrageira
Bactéria Aeróbia
Fermentação Aeróbica
Fermentação Acida
Nutrição Animal
Alimentação
Opuntia
Silage
Silage fermentation
title_short Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
title_full Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
title_fullStr Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
title_full_unstemmed Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
title_sort Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
author PEREIRA, G. A.
author_facet PEREIRA, G. A.
SANTOS, E. M.
ARAUJO, G. G. L. de
OLIVEIRA, J. S.
PINHO, R. M. A
ZANINE, A. de M.
SOUZA, A. F. N.
MACEDO, A. J. S.
NETO, J. M. C.
NASCIMENTO, T. V. C.
author_role author
author2 SANTOS, E. M.
ARAUJO, G. G. L. de
OLIVEIRA, J. S.
PINHO, R. M. A
ZANINE, A. de M.
SOUZA, A. F. N.
MACEDO, A. J. S.
NETO, J. M. C.
NASCIMENTO, T. V. C.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv G. A. Pereira; E. M. Santos; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; J. S. Oliveira; R. M. A. Pinho; A. de M. Zanine; A. F. N. Souza; A. J. S. Macedo; J. M. C. Neto; T. V. C. Nascimento.
dc.contributor.author.fl_str_mv PEREIRA, G. A.
SANTOS, E. M.
ARAUJO, G. G. L. de
OLIVEIRA, J. S.
PINHO, R. M. A
ZANINE, A. de M.
SOUZA, A. F. N.
MACEDO, A. J. S.
NETO, J. M. C.
NASCIMENTO, T. V. C.
dc.subject.por.fl_str_mv Cepas de bactéria de ácido láctico
Fermentação da silagem
Alimentação animal
Silagem
Palma Forrageira
Bactéria Aeróbia
Fermentação Aeróbica
Fermentação Acida
Nutrição Animal
Alimentação
Opuntia
Silage
Silage fermentation
topic Cepas de bactéria de ácido láctico
Fermentação da silagem
Alimentação animal
Silagem
Palma Forrageira
Bactéria Aeróbia
Fermentação Aeróbica
Fermentação Acida
Nutrição Animal
Alimentação
Opuntia
Silage
Silage fermentation
description The current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021-02-18T15:18:10Z
2021-02-18T15:18:10Z
2021-02-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv The Journal of Agricultural Science, v. 157, n. 9-10, p. 684-692, 2020.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082
https:// doi.org/10.1017/S0021859620000143
identifier_str_mv The Journal of Agricultural Science, v. 157, n. 9-10, p. 684-692, 2020.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082
https:// doi.org/10.1017/S0021859620000143
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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