Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082 https:// doi.org/10.1017/S0021859620000143 |
Resumo: | The current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage. |
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Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage.Cepas de bactéria de ácido lácticoFermentação da silagemAlimentação animalSilagemPalma ForrageiraBactéria AeróbiaFermentação AeróbicaFermentação AcidaNutrição AnimalAlimentaçãoOpuntiaSilageSilage fermentationThe current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage.G. A. Pereira; E. M. Santos; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; J. S. Oliveira; R. M. A. Pinho; A. de M. Zanine; A. F. N. Souza; A. J. S. Macedo; J. M. C. Neto; T. V. C. Nascimento.PEREIRA, G. A.SANTOS, E. M.ARAUJO, G. G. L. deOLIVEIRA, J. S.PINHO, R. M. AZANINE, A. de M.SOUZA, A. F. N.MACEDO, A. J. S.NETO, J. M. C.NASCIMENTO, T. V. C.2021-02-18T15:18:10Z2021-02-18T15:18:10Z2021-02-182020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe Journal of Agricultural Science, v. 157, n. 9-10, p. 684-692, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082https:// doi.org/10.1017/S0021859620000143enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-02-18T15:18:20Zoai:www.alice.cnptia.embrapa.br:doc/1130082Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-02-18T15:18:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-02-18T15:18:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
title |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
spellingShingle |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. PEREIRA, G. A. Cepas de bactéria de ácido láctico Fermentação da silagem Alimentação animal Silagem Palma Forrageira Bactéria Aeróbia Fermentação Aeróbica Fermentação Acida Nutrição Animal Alimentação Opuntia Silage Silage fermentation |
title_short |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
title_full |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
title_fullStr |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
title_full_unstemmed |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
title_sort |
Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage. |
author |
PEREIRA, G. A. |
author_facet |
PEREIRA, G. A. SANTOS, E. M. ARAUJO, G. G. L. de OLIVEIRA, J. S. PINHO, R. M. A ZANINE, A. de M. SOUZA, A. F. N. MACEDO, A. J. S. NETO, J. M. C. NASCIMENTO, T. V. C. |
author_role |
author |
author2 |
SANTOS, E. M. ARAUJO, G. G. L. de OLIVEIRA, J. S. PINHO, R. M. A ZANINE, A. de M. SOUZA, A. F. N. MACEDO, A. J. S. NETO, J. M. C. NASCIMENTO, T. V. C. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
G. A. Pereira; E. M. Santos; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; J. S. Oliveira; R. M. A. Pinho; A. de M. Zanine; A. F. N. Souza; A. J. S. Macedo; J. M. C. Neto; T. V. C. Nascimento. |
dc.contributor.author.fl_str_mv |
PEREIRA, G. A. SANTOS, E. M. ARAUJO, G. G. L. de OLIVEIRA, J. S. PINHO, R. M. A ZANINE, A. de M. SOUZA, A. F. N. MACEDO, A. J. S. NETO, J. M. C. NASCIMENTO, T. V. C. |
dc.subject.por.fl_str_mv |
Cepas de bactéria de ácido láctico Fermentação da silagem Alimentação animal Silagem Palma Forrageira Bactéria Aeróbia Fermentação Aeróbica Fermentação Acida Nutrição Animal Alimentação Opuntia Silage Silage fermentation |
topic |
Cepas de bactéria de ácido láctico Fermentação da silagem Alimentação animal Silagem Palma Forrageira Bactéria Aeróbia Fermentação Aeróbica Fermentação Acida Nutrição Animal Alimentação Opuntia Silage Silage fermentation |
description |
The current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2021-02-18T15:18:10Z 2021-02-18T15:18:10Z 2021-02-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
The Journal of Agricultural Science, v. 157, n. 9-10, p. 684-692, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082 https:// doi.org/10.1017/S0021859620000143 |
identifier_str_mv |
The Journal of Agricultural Science, v. 157, n. 9-10, p. 684-692, 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130082 https:// doi.org/10.1017/S0021859620000143 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503502796423168 |