Making a better home: modulation of plant defensive response by brevipalpus mites.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095414 |
Resumo: | False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growthdefense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the noninfested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JAmediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway. |
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Making a better home: modulation of plant defensive response by brevipalpus mites.HerbivoresPlant hormonesSalicylic acidJasmonic acidFalse-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growthdefense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the noninfested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JAmediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway.GABRIELLA D. ARENA, Instituto Agronômico de Campinas; PEDRO L. RAMOS-GONZÁLEZ, Universidade Estadual de Campinas; LUANA A. ROGERIO, Instituto Agronômico de Campinas; MARCELO RIBEIRO-ALVES, Fundação Oswaldo Cruz; CLARE L. CASTEEL, University of California; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Instituto Agronômico de Campinas.ARENA, G. D.RAMOS-GONZÁLEZ, P. L.ROGERIO, L. A.RIBEIRO-ALVES, M.CASTEEL, C. L.ASTUA, J. de F.MACHADO, M. A.2018-09-12T00:35:57Z2018-09-12T00:35:57Z2018-09-1120182018-10-31T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Plant Science, v.9, August, 2018.1664-462Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/109541410.3389/fpls.2018.01147enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-09-12T00:36:04Zoai:www.alice.cnptia.embrapa.br:doc/1095414Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-09-12T00:36:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-09-12T00:36:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
title |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
spellingShingle |
Making a better home: modulation of plant defensive response by brevipalpus mites. ARENA, G. D. Herbivores Plant hormones Salicylic acid Jasmonic acid |
title_short |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
title_full |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
title_fullStr |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
title_full_unstemmed |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
title_sort |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
author |
ARENA, G. D. |
author_facet |
ARENA, G. D. RAMOS-GONZÁLEZ, P. L. ROGERIO, L. A. RIBEIRO-ALVES, M. CASTEEL, C. L. ASTUA, J. de F. MACHADO, M. A. |
author_role |
author |
author2 |
RAMOS-GONZÁLEZ, P. L. ROGERIO, L. A. RIBEIRO-ALVES, M. CASTEEL, C. L. ASTUA, J. de F. MACHADO, M. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
GABRIELLA D. ARENA, Instituto Agronômico de Campinas; PEDRO L. RAMOS-GONZÁLEZ, Universidade Estadual de Campinas; LUANA A. ROGERIO, Instituto Agronômico de Campinas; MARCELO RIBEIRO-ALVES, Fundação Oswaldo Cruz; CLARE L. CASTEEL, University of California; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Instituto Agronômico de Campinas. |
dc.contributor.author.fl_str_mv |
ARENA, G. D. RAMOS-GONZÁLEZ, P. L. ROGERIO, L. A. RIBEIRO-ALVES, M. CASTEEL, C. L. ASTUA, J. de F. MACHADO, M. A. |
dc.subject.por.fl_str_mv |
Herbivores Plant hormones Salicylic acid Jasmonic acid |
topic |
Herbivores Plant hormones Salicylic acid Jasmonic acid |
description |
False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growthdefense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the noninfested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JAmediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-12T00:35:57Z 2018-09-12T00:35:57Z 2018-09-11 2018 2018-10-31T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Plant Science, v.9, August, 2018. 1664-462X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095414 10.3389/fpls.2018.01147 |
identifier_str_mv |
Frontiers in Plant Science, v.9, August, 2018. 1664-462X 10.3389/fpls.2018.01147 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095414 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503462135791616 |