Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.

Detalhes bibliográficos
Autor(a) principal: ALMEIDA, I. G.
Data de Publicação: 2013
Outros Autores: IANELLA, P., FARIA, M. T., PAIVA, S. R., CAETANO, A. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/982325
http://dx.doi.org/10.4238/2013.December.4.17
Resumo: Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A. gigas male and female DNA pools with 566 RAPD primers, generating 2609 fragments, with an estimated 1341 segregating polymorphic markers, and an estimated average spacing of 714 kb, which corresponds to less than 0.1% of the species' genome. Two putative sex-specific fragments were initially identified in bulked samples; but they were not confirmed in a study of individual male and female samples. We suggest that A. gigas has developed a non-chromosomal system of sex determination or, alternatively, that the species has undergone a recent loss of the chromosome carrying the sex-determining locus.
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spelling Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.Marcadores molecularesPeixe neotropicalRAPDGenomaPirarucuArapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A. gigas male and female DNA pools with 566 RAPD primers, generating 2609 fragments, with an estimated 1341 segregating polymorphic markers, and an estimated average spacing of 714 kb, which corresponds to less than 0.1% of the species' genome. Two putative sex-specific fragments were initially identified in bulked samples; but they were not confirmed in a study of individual male and female samples. We suggest that A. gigas has developed a non-chromosomal system of sex determination or, alternatively, that the species has undergone a recent loss of the chromosome carrying the sex-determining locus.Programa de Pós-Graduação em Ciências Animais, Universidade de Brasília; PATRICIA IANELLA, DPD; MARCOS TUCUNDUVA DE FARIA, CPATU; SAMUEL REZENDE PAIVA, CENARGEN; ALEXANDRE RODRIGUES CAETANO, CENARGEN.ALMEIDA, I. G.IANELLA, P.FARIA, M. T.PAIVA, S. R.CAETANO, A. R.2014-03-14T11:11:11Z2014-03-14T11:11:11Z2014-03-1420132015-04-07T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 12, n. 4, p. 6299-6308, Dec. 2013.http://www.alice.cnptia.embrapa.br/alice/handle/doc/982325http://dx.doi.org/10.4238/2013.December.4.17enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:18:15Zoai:www.alice.cnptia.embrapa.br:doc/982325Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:18:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:18:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
title Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
spellingShingle Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
ALMEIDA, I. G.
Marcadores moleculares
Peixe neotropical
RAPD
Genoma
Pirarucu
title_short Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
title_full Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
title_fullStr Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
title_full_unstemmed Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
title_sort Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.
author ALMEIDA, I. G.
author_facet ALMEIDA, I. G.
IANELLA, P.
FARIA, M. T.
PAIVA, S. R.
CAETANO, A. R.
author_role author
author2 IANELLA, P.
FARIA, M. T.
PAIVA, S. R.
CAETANO, A. R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Programa de Pós-Graduação em Ciências Animais, Universidade de Brasília; PATRICIA IANELLA, DPD; MARCOS TUCUNDUVA DE FARIA, CPATU; SAMUEL REZENDE PAIVA, CENARGEN; ALEXANDRE RODRIGUES CAETANO, CENARGEN.
dc.contributor.author.fl_str_mv ALMEIDA, I. G.
IANELLA, P.
FARIA, M. T.
PAIVA, S. R.
CAETANO, A. R.
dc.subject.por.fl_str_mv Marcadores moleculares
Peixe neotropical
RAPD
Genoma
Pirarucu
topic Marcadores moleculares
Peixe neotropical
RAPD
Genoma
Pirarucu
description Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A. gigas male and female DNA pools with 566 RAPD primers, generating 2609 fragments, with an estimated 1341 segregating polymorphic markers, and an estimated average spacing of 714 kb, which corresponds to less than 0.1% of the species' genome. Two putative sex-specific fragments were initially identified in bulked samples; but they were not confirmed in a study of individual male and female samples. We suggest that A. gigas has developed a non-chromosomal system of sex determination or, alternatively, that the species has undergone a recent loss of the chromosome carrying the sex-determining locus.
publishDate 2013
dc.date.none.fl_str_mv 2013
2014-03-14T11:11:11Z
2014-03-14T11:11:11Z
2014-03-14
2015-04-07T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, v. 12, n. 4, p. 6299-6308, Dec. 2013.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/982325
http://dx.doi.org/10.4238/2013.December.4.17
identifier_str_mv Genetics and Molecular Research, v. 12, n. 4, p. 6299-6308, Dec. 2013.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/982325
http://dx.doi.org/10.4238/2013.December.4.17
dc.language.iso.fl_str_mv eng
language eng
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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