Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/39532 |
Resumo: | The Pirarucu (Arapaima gigas) is one of the world’s largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air breather found in the basin of the Amazon River with an attractive potential for aquaculture. Its phylogenetic position among bony fishes makes the Pirarucu a relevant subject for evolutionary studies of early teleost diversification. Here, we present, for the first time, a draft genome version of the A. gigas genome, providing useful information for further functional and evolutionary studies. The A. gigas genome was assembled with 103-Gb raw reads sequenced in an Illumina platform. The final draft genome assembly was 661 Mb, with a contig N50 equal to 51.23 kb and scaffold N50 of 668 kb. Repeat sequences accounted for 21.69% of the whole genome, and a total of 24,655 protein-coding genes were predicted from the genome assembly, with an average of nine exons per gene. Phylogenomic analysis based on 24 fish species supported the postulation that Osteoglossomorpha and Elopomorpha (eels, tarpons, and bonefishes) are sister groups, both forming a sister lineage with respect to Clupeocephala (remaining teleosts). Divergence time estimations suggested that Osteoglossomorpha and Elopomorpha lineages emerged independently in a period of 30 Myr in the Jurassic. The draft genome of A. gigas provides a valuable genetic resource for further investigations of evolutionary studies and may also offer a valuable data for economic applications. |
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2022-02-21T19:53:44Z2022-02-21T19:53:44Z2018-07-051092366237910.1093/gbe/evy13017596653http://hdl.handle.net/1843/39532The Pirarucu (Arapaima gigas) is one of the world’s largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air breather found in the basin of the Amazon River with an attractive potential for aquaculture. Its phylogenetic position among bony fishes makes the Pirarucu a relevant subject for evolutionary studies of early teleost diversification. Here, we present, for the first time, a draft genome version of the A. gigas genome, providing useful information for further functional and evolutionary studies. The A. gigas genome was assembled with 103-Gb raw reads sequenced in an Illumina platform. The final draft genome assembly was 661 Mb, with a contig N50 equal to 51.23 kb and scaffold N50 of 668 kb. Repeat sequences accounted for 21.69% of the whole genome, and a total of 24,655 protein-coding genes were predicted from the genome assembly, with an average of nine exons per gene. Phylogenomic analysis based on 24 fish species supported the postulation that Osteoglossomorpha and Elopomorpha (eels, tarpons, and bonefishes) are sister groups, both forming a sister lineage with respect to Clupeocephala (remaining teleosts). Divergence time estimations suggested that Osteoglossomorpha and Elopomorpha lineages emerged independently in a period of 30 Myr in the Jurassic. The draft genome of A. gigas provides a valuable genetic resource for further investigations of evolutionary studies and may also offer a valuable data for economic applications.O Pirarucu (Arapaima gigas) é um dos maiores peixes de água doce do mundo e membro da superordem Osteoglossomorpha (bonytongues), uma das mais antigas linhagens de peixes raiados. Esta espécie é um respirador aéreo obrigatório encontrado na bacia do rio Amazonas com um potencial atrativo para a aquicultura. Sua posição filogenética entre os peixes ósseos torna o Pirarucu um assunto relevante para estudos evolutivos da diversificação dos primeiros teleósteos. Aqui nós apresentar, pela primeira vez, uma versão preliminar do genoma do A. gigas, fornecendo informações úteis para e estudos evolutivos. O genoma de A. gigas foi montado com leituras brutas de 103 Gb sequenciadas em uma plataforma Illumina. O projeto final de montagem do genoma foi de 661 Mb, com um contig N50 igual a 51,23 kb e scaffold N50 de 668 kb. Repita sequências representaram 21,69% de todo o genoma, e um total de 24.655 genes codificadores de proteínas foram previstos a partir de a montagem do genoma, com uma média de nove éxons por gene. A análise filogenômica baseada em 24 espécies de peixes apoiou a postulação de que Osteoglossomorpha e Elopomorpha (enguias, tarpons e bonefishes) são grupos irmãos, ambos formando uma linhagem irmã em relação a Clupeocephala (teleósteos restantes). As estimativas de tempo de divergência sugeriram que As linhagens Osteoglossomorpha e Elopomorpha surgiram independentemente em um período de 30 Myr no Jurássico. O rascunho genoma de A. gigas fornece um valioso recurso genético para futuras investigações de estudos evolutivos e também pode oferecer um dado valioso para aplicações econômicas.engUniversidade Federal de Minas GeraisUFMGBrasilICB - INSTITUTO DE CIÊNCIAS BIOLOGICASGenome biology and evolutionArapaima gigasPirarucuOsteoglossomorphaTeleosteiSequenciamento de genomaFilogeniaArapaima gigasPirarucuOsteoglossomorphaTeleosteigenome sequencingphylogenomicsWhole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost cladesSequenciamento completo do genoma do pirarucu (arapaima gigas) suporta a emergência independente dos principais clados de teleósteosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRicardo Assunção VialleEdivaldo Herculano Corrêa de OliveiraIgor Guerreiro HamoyPaulo Pimentel AssumpçãoÂndrea Ribeiro-dos-santosJoão Paulo Matos Santos LimaHéctor n SeuánezSandro José de SouzaSidney SantosJorge Estefano Santana de SouzaKatia de Paiva LopesDiego Gomes TeixeiraPitágoras de Azevedo Alves SobrinhoAndré m Ribeiro-dos-santosCarolina FurtadoTetsu SakamotoFábio Augusto Oliveira Silvaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/39532/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALWhole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades.pdfWhole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades.pdfapplication/pdf985645https://repositorio.ufmg.br/bitstream/1843/39532/2/Whole%20genome%20sequencing%20of%20the%20pirarucu%20%28arapaima%20gigas%29%20supports%20independent%20emergence%20of%20major%20teleost%20clades.pdf39abe689d2a59a44abe0618c08351952MD521843/395322022-02-21 16:53:44.162oai:repositorio.ufmg.br:1843/39532TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-02-21T19:53:44Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
dc.title.alternative.pt_BR.fl_str_mv |
Sequenciamento completo do genoma do pirarucu (arapaima gigas) suporta a emergência independente dos principais clados de teleósteos |
title |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
spellingShingle |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades Ricardo Assunção Vialle Arapaima gigas Pirarucu Osteoglossomorpha Teleostei genome sequencing phylogenomics Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Sequenciamento de genoma Filogenia |
title_short |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
title_full |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
title_fullStr |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
title_full_unstemmed |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
title_sort |
Whole genome sequencing of the pirarucu (arapaima gigas) supports independent emergence of major teleost clades |
author |
Ricardo Assunção Vialle |
author_facet |
Ricardo Assunção Vialle Edivaldo Herculano Corrêa de Oliveira Igor Guerreiro Hamoy Paulo Pimentel Assumpção Ândrea Ribeiro-dos-santos João Paulo Matos Santos Lima Héctor n Seuánez Sandro José de Souza Sidney Santos Jorge Estefano Santana de Souza Katia de Paiva Lopes Diego Gomes Teixeira Pitágoras de Azevedo Alves Sobrinho André m Ribeiro-dos-santos Carolina Furtado Tetsu Sakamoto Fábio Augusto Oliveira Silva |
author_role |
author |
author2 |
Edivaldo Herculano Corrêa de Oliveira Igor Guerreiro Hamoy Paulo Pimentel Assumpção Ândrea Ribeiro-dos-santos João Paulo Matos Santos Lima Héctor n Seuánez Sandro José de Souza Sidney Santos Jorge Estefano Santana de Souza Katia de Paiva Lopes Diego Gomes Teixeira Pitágoras de Azevedo Alves Sobrinho André m Ribeiro-dos-santos Carolina Furtado Tetsu Sakamoto Fábio Augusto Oliveira Silva |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ricardo Assunção Vialle Edivaldo Herculano Corrêa de Oliveira Igor Guerreiro Hamoy Paulo Pimentel Assumpção Ândrea Ribeiro-dos-santos João Paulo Matos Santos Lima Héctor n Seuánez Sandro José de Souza Sidney Santos Jorge Estefano Santana de Souza Katia de Paiva Lopes Diego Gomes Teixeira Pitágoras de Azevedo Alves Sobrinho André m Ribeiro-dos-santos Carolina Furtado Tetsu Sakamoto Fábio Augusto Oliveira Silva |
dc.subject.por.fl_str_mv |
Arapaima gigas Pirarucu Osteoglossomorpha Teleostei genome sequencing phylogenomics |
topic |
Arapaima gigas Pirarucu Osteoglossomorpha Teleostei genome sequencing phylogenomics Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Sequenciamento de genoma Filogenia |
dc.subject.other.pt_BR.fl_str_mv |
Arapaima gigas Pirarucu Osteoglossomorpha Teleostei Sequenciamento de genoma Filogenia |
description |
The Pirarucu (Arapaima gigas) is one of the world’s largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air breather found in the basin of the Amazon River with an attractive potential for aquaculture. Its phylogenetic position among bony fishes makes the Pirarucu a relevant subject for evolutionary studies of early teleost diversification. Here, we present, for the first time, a draft genome version of the A. gigas genome, providing useful information for further functional and evolutionary studies. The A. gigas genome was assembled with 103-Gb raw reads sequenced in an Illumina platform. The final draft genome assembly was 661 Mb, with a contig N50 equal to 51.23 kb and scaffold N50 of 668 kb. Repeat sequences accounted for 21.69% of the whole genome, and a total of 24,655 protein-coding genes were predicted from the genome assembly, with an average of nine exons per gene. Phylogenomic analysis based on 24 fish species supported the postulation that Osteoglossomorpha and Elopomorpha (eels, tarpons, and bonefishes) are sister groups, both forming a sister lineage with respect to Clupeocephala (remaining teleosts). Divergence time estimations suggested that Osteoglossomorpha and Elopomorpha lineages emerged independently in a period of 30 Myr in the Jurassic. The draft genome of A. gigas provides a valuable genetic resource for further investigations of evolutionary studies and may also offer a valuable data for economic applications. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-07-05 |
dc.date.accessioned.fl_str_mv |
2022-02-21T19:53:44Z |
dc.date.available.fl_str_mv |
2022-02-21T19:53:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/39532 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1093/gbe/evy130 |
dc.identifier.issn.pt_BR.fl_str_mv |
17596653 |
identifier_str_mv |
10.1093/gbe/evy130 17596653 |
url |
http://hdl.handle.net/1843/39532 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genome biology and evolution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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