Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.

Detalhes bibliográficos
Autor(a) principal: BOMFETI, C. A.
Data de Publicação: 2008
Outros Autores: SOUZA-PACCOLA, E. A., MASSOLA JUNIOR, N. S., MARRIEL, I. E., MEIRELLES, W. F., CASELA, C. R., PACCOLA-MEIRELLES, L. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/491172
Resumo: The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
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spelling Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.Doença bacteriana do milhodoenças foliares do milhoMaize bacterial diseaseMaize leaf diseaseMilhoZea MaysThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL.BOMFETI, C. A.SOUZA-PACCOLA, E. A.MASSOLA JUNIOR, N. S.MARRIEL, I. E.MEIRELLES, W. F.CASELA, C. R.PACCOLA-MEIRELLES, L. D.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2008-06-1720082018-05-24T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008.http://www.alice.cnptia.embrapa.br/alice/handle/doc/49117210.1590/S1982-56762008000100010enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:20:51Zoai:www.alice.cnptia.embrapa.br:doc/491172Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:20:51falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:20:51Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
title Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
spellingShingle Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
BOMFETI, C. A.
Doença bacteriana do milho
doenças foliares do milho
Maize bacterial disease
Maize leaf disease
Milho
Zea Mays
title_short Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
title_full Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
title_fullStr Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
title_full_unstemmed Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
title_sort Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
author BOMFETI, C. A.
author_facet BOMFETI, C. A.
SOUZA-PACCOLA, E. A.
MASSOLA JUNIOR, N. S.
MARRIEL, I. E.
MEIRELLES, W. F.
CASELA, C. R.
PACCOLA-MEIRELLES, L. D.
author_role author
author2 SOUZA-PACCOLA, E. A.
MASSOLA JUNIOR, N. S.
MARRIEL, I. E.
MEIRELLES, W. F.
CASELA, C. R.
PACCOLA-MEIRELLES, L. D.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL.
dc.contributor.author.fl_str_mv BOMFETI, C. A.
SOUZA-PACCOLA, E. A.
MASSOLA JUNIOR, N. S.
MARRIEL, I. E.
MEIRELLES, W. F.
CASELA, C. R.
PACCOLA-MEIRELLES, L. D.
dc.subject.por.fl_str_mv Doença bacteriana do milho
doenças foliares do milho
Maize bacterial disease
Maize leaf disease
Milho
Zea Mays
topic Doença bacteriana do milho
doenças foliares do milho
Maize bacterial disease
Maize leaf disease
Milho
Zea Mays
description The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-17
2008
2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2018-05-24T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/491172
10.1590/S1982-56762008000100010
identifier_str_mv Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008.
10.1590/S1982-56762008000100010
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/491172
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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