Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.

• Autor(a) principal: MOURA, R. R.
• Data de Publicação: 2014
• Outros Autores: SOUZA-FABJAN, J. M. G., FONSECA, J. F. da, MELO, C. H. S., SANCHEZ, D. J. D., VIEIRA, M. P., ALMEIDA, T. M., SEROVA, I. A., SEROV, O. L., PEREIRA, A. F., TEIXEIRA, D. I. A., MELO, L. M., FREITAS, V. J. F.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516
• topic: Transgenia; Goat; Caprino; Cabra; Transferência de embrião; Hormônio animal; Progesterona; Superovulação; Embryo transfer; Progesterone; Superovulation; Transgenesis

Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.