Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.

Detalhes bibliográficos
Autor(a) principal: ARAÚJO, E. R.
Data de Publicação: 2012
Outros Autores: COSTA, J. R., FERREIRA, M. A. S. V., QUEZADO-DUVAL, A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/934722
Resumo: To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.
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spelling Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.DetecçãoPCRBactériaDoençaIdentificaçãoXanthomonasTo establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.ALICE MARIA QUEZADO DUVAL, CNPH.ARAÚJO, E. R.COSTA, J. R.FERREIRA, M. A. S. V.QUEZADO-DUVAL, A. M.2018-05-25T00:32:02Z2018-05-25T00:32:02Z2012-09-2720122018-05-25T00:32:02Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Applied Microbiology, Oxford, Sept. 2012.1364-5072http://www.alice.cnptia.embrapa.br/alice/handle/doc/93472210.1111/j.1365-2672.05431.xenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-05-25T00:32:09Zoai:www.alice.cnptia.embrapa.br:doc/934722Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-05-25T00:32:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
title Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
spellingShingle Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
ARAÚJO, E. R.
Detecção
PCR
Bactéria
Doença
Identificação
Xanthomonas
title_short Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
title_full Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
title_fullStr Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
title_full_unstemmed Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
title_sort Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
author ARAÚJO, E. R.
author_facet ARAÚJO, E. R.
COSTA, J. R.
FERREIRA, M. A. S. V.
QUEZADO-DUVAL, A. M.
author_role author
author2 COSTA, J. R.
FERREIRA, M. A. S. V.
QUEZADO-DUVAL, A. M.
author2_role author
author
author
dc.contributor.none.fl_str_mv ALICE MARIA QUEZADO DUVAL, CNPH.
dc.contributor.author.fl_str_mv ARAÚJO, E. R.
COSTA, J. R.
FERREIRA, M. A. S. V.
QUEZADO-DUVAL, A. M.
dc.subject.por.fl_str_mv Detecção
PCR
Bactéria
Doença
Identificação
Xanthomonas
topic Detecção
PCR
Bactéria
Doença
Identificação
Xanthomonas
description To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-27
2012
2018-05-25T00:32:02Z
2018-05-25T00:32:02Z
2018-05-25T00:32:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of Applied Microbiology, Oxford, Sept. 2012.
1364-5072
http://www.alice.cnptia.embrapa.br/alice/handle/doc/934722
10.1111/j.1365-2672.05431.x
identifier_str_mv Journal of Applied Microbiology, Oxford, Sept. 2012.
1364-5072
10.1111/j.1365-2672.05431.x
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/934722
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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