High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/162807 |
Resumo: | Euchroma giganteawas karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform suernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. giganteamay have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. Small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. The sex chromosomes showed larger constitutive heterochromatin blocks. Silver nitrate staining during prophase I of meiosis showed labeling of the sex chromosome chain. However, the rDNA sites could only be precisely determined by FISH, which permitted the identification of these ribosomal sites on chromosomes X1 and X2 of this species. |
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High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)CromossomoRONDNArColeópteroEuchroma giganteawas karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform suernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. giganteamay have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. Small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. The sex chromosomes showed larger constitutive heterochromatin blocks. Silver nitrate staining during prophase I of meiosis showed labeling of the sex chromosome chain. However, the rDNA sites could only be precisely determined by FISH, which permitted the identification of these ribosomal sites on chromosomes X1 and X2 of this species.Rita de Cássia de Moura, UFPENATONIEL FRANKLIN DE MELO, CPATSAMaria José de Souza, UFPE.MOURA, R. de C. deMELO, N. F. deSOUZA, M. J. de2011-04-09T20:52:52Z2011-04-09T20:52:52Z2008-06-0520082018-09-03T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 431-437, 2008.http://www.alice.cnptia.embrapa.br/alice/handle/doc/162807enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:24:31Zoai:www.alice.cnptia.embrapa.br:doc/162807Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:24:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:24:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
title |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
spellingShingle |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) MOURA, R. de C. de Cromossomo RON DNAr Coleóptero |
title_short |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
title_full |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
title_fullStr |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
title_full_unstemmed |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
title_sort |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) |
author |
MOURA, R. de C. de |
author_facet |
MOURA, R. de C. de MELO, N. F. de SOUZA, M. J. de |
author_role |
author |
author2 |
MELO, N. F. de SOUZA, M. J. de |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Rita de Cássia de Moura, UFPE NATONIEL FRANKLIN DE MELO, CPATSA Maria José de Souza, UFPE. |
dc.contributor.author.fl_str_mv |
MOURA, R. de C. de MELO, N. F. de SOUZA, M. J. de |
dc.subject.por.fl_str_mv |
Cromossomo RON DNAr Coleóptero |
topic |
Cromossomo RON DNAr Coleóptero |
description |
Euchroma giganteawas karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform suernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. giganteamay have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. Small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. The sex chromosomes showed larger constitutive heterochromatin blocks. Silver nitrate staining during prophase I of meiosis showed labeling of the sex chromosome chain. However, the rDNA sites could only be precisely determined by FISH, which permitted the identification of these ribosomal sites on chromosomes X1 and X2 of this species. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-05 2008 2011-04-09T20:52:52Z 2011-04-09T20:52:52Z 2018-09-03T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 431-437, 2008. http://www.alice.cnptia.embrapa.br/alice/handle/doc/162807 |
identifier_str_mv |
Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 431-437, 2008. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/162807 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503335405944832 |