Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129569 |
Resumo: | Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe?P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth. |
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Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo.BactériaFosfatoSolubilizaçãoMilhoEstimulante de Crescimento VegetalRock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe?P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth.UBIANA C. SILVA, Universidade Federal de Minas GeraisSARA CUADROS-ORELLANA, Universidad Católica del Maule, Talca, ChileDALIANE R. C. SILVA, Universidade Federal de Minas GeraisLUIZ F. FREITAS-JÚNIOR, Universidade Federal de Minas GeraisANA C. FERNANDES, Universidade Federal de Minas GeraisLAURA R. LEITE, Fiocruz, Belo HorizonteCHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMSVERA L. DOS SANTOS, Universidade Federal de Minas Gerais.SILVA, U. C.CUADROS-ORELLANA, S.SILVA, D. R. C.FREITAS-JÚNIOR, L. F.FERNANDES. A. C.LEITE, L. R.OLIVEIRA-PAIVA, C. A.SANTOS, V. L. dos2021-01-28T09:04:32Z2021-01-28T09:04:32Z2021-01-272021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Microbiology, v. 11, article 574550, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112956910.3389/fmicb.2020.574550enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-01-28T09:04:39Zoai:www.alice.cnptia.embrapa.br:doc/1129569Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-01-28T09:04:39Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
title |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
spellingShingle |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. SILVA, U. C. Bactéria Fosfato Solubilização Milho Estimulante de Crescimento Vegetal |
title_short |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
title_full |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
title_fullStr |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
title_full_unstemmed |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
title_sort |
Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo. |
author |
SILVA, U. C. |
author_facet |
SILVA, U. C. CUADROS-ORELLANA, S. SILVA, D. R. C. FREITAS-JÚNIOR, L. F. FERNANDES. A. C. LEITE, L. R. OLIVEIRA-PAIVA, C. A. SANTOS, V. L. dos |
author_role |
author |
author2 |
CUADROS-ORELLANA, S. SILVA, D. R. C. FREITAS-JÚNIOR, L. F. FERNANDES. A. C. LEITE, L. R. OLIVEIRA-PAIVA, C. A. SANTOS, V. L. dos |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
UBIANA C. SILVA, Universidade Federal de Minas Gerais SARA CUADROS-ORELLANA, Universidad Católica del Maule, Talca, Chile DALIANE R. C. SILVA, Universidade Federal de Minas Gerais LUIZ F. FREITAS-JÚNIOR, Universidade Federal de Minas Gerais ANA C. FERNANDES, Universidade Federal de Minas Gerais LAURA R. LEITE, Fiocruz, Belo Horizonte CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS VERA L. DOS SANTOS, Universidade Federal de Minas Gerais. |
dc.contributor.author.fl_str_mv |
SILVA, U. C. CUADROS-ORELLANA, S. SILVA, D. R. C. FREITAS-JÚNIOR, L. F. FERNANDES. A. C. LEITE, L. R. OLIVEIRA-PAIVA, C. A. SANTOS, V. L. dos |
dc.subject.por.fl_str_mv |
Bactéria Fosfato Solubilização Milho Estimulante de Crescimento Vegetal |
topic |
Bactéria Fosfato Solubilização Milho Estimulante de Crescimento Vegetal |
description |
Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe?P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-28T09:04:32Z 2021-01-28T09:04:32Z 2021-01-27 2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Microbiology, v. 11, article 574550, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129569 10.3389/fmicb.2020.574550 |
identifier_str_mv |
Frontiers in Microbiology, v. 11, article 574550, 2021. 10.3389/fmicb.2020.574550 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129569 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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