A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.

Detalhes bibliográficos
Autor(a) principal: MARTINS, P. K.
Data de Publicação: 2015
Outros Autores: RIBEIRO, A. P., DIAS, B. B. A., KOBAYASHI, A. K., MOLINARI, H. B. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1025487
https://doi.org/10.1016/j.btre.2015.02.002
Resumo: Abstract: The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane
id EMBR_e6307831d1466faef21d7c2946a50841
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/1025487
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.Model systemC4 metabolismGreen milletCana de AçúcarBioenergiaMilho VerdeGenomaBioenergySugarcaneSetaria viridisGenomicsAbstract: The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcanePolyana Kelly Martins; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.MARTINS, P. K.RIBEIRO, A. P.DIAS, B. B. A.KOBAYASHI, A. K.MOLINARI, H. B. C.2021-04-06T12:33:55Z2021-04-06T12:33:55Z2015-09-302015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlep. 41-44Biotechnology Reports, v. 6, p. 41-44, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1025487https://doi.org/10.1016/j.btre.2015.02.002enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-04-06T12:34:05Zoai:www.alice.cnptia.embrapa.br:doc/1025487Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-04-06T12:34:05falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-04-06T12:34:05Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
title A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
spellingShingle A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
MARTINS, P. K.
Model system
C4 metabolism
Green millet
Cana de Açúcar
Bioenergia
Milho Verde
Genoma
Bioenergy
Sugarcane
Setaria viridis
Genomics
title_short A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
title_full A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
title_fullStr A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
title_full_unstemmed A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
title_sort A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
author MARTINS, P. K.
author_facet MARTINS, P. K.
RIBEIRO, A. P.
DIAS, B. B. A.
KOBAYASHI, A. K.
MOLINARI, H. B. C.
author_role author
author2 RIBEIRO, A. P.
DIAS, B. B. A.
KOBAYASHI, A. K.
MOLINARI, H. B. C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Polyana Kelly Martins; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.
dc.contributor.author.fl_str_mv MARTINS, P. K.
RIBEIRO, A. P.
DIAS, B. B. A.
KOBAYASHI, A. K.
MOLINARI, H. B. C.
dc.subject.por.fl_str_mv Model system
C4 metabolism
Green millet
Cana de Açúcar
Bioenergia
Milho Verde
Genoma
Bioenergy
Sugarcane
Setaria viridis
Genomics
topic Model system
C4 metabolism
Green millet
Cana de Açúcar
Bioenergia
Milho Verde
Genoma
Bioenergy
Sugarcane
Setaria viridis
Genomics
description Abstract: The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane
publishDate 2015
dc.date.none.fl_str_mv 2015-09-30
2015
2021-04-06T12:33:55Z
2021-04-06T12:33:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Biotechnology Reports, v. 6, p. 41-44, 2015.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1025487
https://doi.org/10.1016/j.btre.2015.02.002
identifier_str_mv Biotechnology Reports, v. 6, p. 41-44, 2015.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1025487
https://doi.org/10.1016/j.btre.2015.02.002
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv p. 41-44
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503503953002496

Registros relacionados