Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/973974 |
Resumo: | The most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania, and S. albula) and R. nu from C. includens. However, Bpm I did not digest the amplicons from S. eridania and C. includens, which were discriminated by RFLP patterns produced by the restriction enzyme MboI. PCR-RFLP can be performed in a short period, and it is useful to distinguish the most important Brazilian Lepidoptera soybean pests. |
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Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil.LepidoteroSojaPraga de plantaPhytophthoraPseudoplusia IncludensSpodoptera EridaniaLepidopteraRachiplusiaSpodoptera albulaPlant pestsSoybeansBrazilSpodoptera cosmioidesThe most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania, and S. albula) and R. nu from C. includens. However, Bpm I did not digest the amplicons from S. eridania and C. includens, which were discriminated by RFLP patterns produced by the restriction enzyme MboI. PCR-RFLP can be performed in a short period, and it is useful to distinguish the most important Brazilian Lepidoptera soybean pests.ADRIAN AUGUSTO SOSA GOMEZ ROLIM, ESALQ - USP; SILVIA AKIMI CAVAGUCHI YANO, CNPSo; ALEXANDRE SPECHT, CPAC; CELIA GUADALUPE TARDELLI DE JESUS ANDRADE, UEL; DANIEL RICARDO SOSA GOMEZ, CNPSO.ROLIM, A. A. S. G.YANO, S. A. C.SPECHT, A.ANDRADE, C. G. T. de J.SOSA-GOMEZ, D. R.2013-12-16T22:30:00Z2013-12-16T22:30:00Z2013-12-1620132013-12-16T22:30:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnnals of the Entomological Society of America, v. 106, n. 5, p. 643-651, 2013.http://www.alice.cnptia.embrapa.br/alice/handle/doc/97397410.1603/AN13049enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:29:07Zoai:www.alice.cnptia.embrapa.br:doc/973974Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:29:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
title |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
spellingShingle |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. ROLIM, A. A. S. G. Lepidotero Soja Praga de planta Phytophthora Pseudoplusia Includens Spodoptera Eridania Lepidoptera Rachiplusia Spodoptera albula Plant pests Soybeans Brazil Spodoptera cosmioides |
title_short |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
title_full |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
title_fullStr |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
title_full_unstemmed |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
title_sort |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
author |
ROLIM, A. A. S. G. |
author_facet |
ROLIM, A. A. S. G. YANO, S. A. C. SPECHT, A. ANDRADE, C. G. T. de J. SOSA-GOMEZ, D. R. |
author_role |
author |
author2 |
YANO, S. A. C. SPECHT, A. ANDRADE, C. G. T. de J. SOSA-GOMEZ, D. R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
ADRIAN AUGUSTO SOSA GOMEZ ROLIM, ESALQ - USP; SILVIA AKIMI CAVAGUCHI YANO, CNPSo; ALEXANDRE SPECHT, CPAC; CELIA GUADALUPE TARDELLI DE JESUS ANDRADE, UEL; DANIEL RICARDO SOSA GOMEZ, CNPSO. |
dc.contributor.author.fl_str_mv |
ROLIM, A. A. S. G. YANO, S. A. C. SPECHT, A. ANDRADE, C. G. T. de J. SOSA-GOMEZ, D. R. |
dc.subject.por.fl_str_mv |
Lepidotero Soja Praga de planta Phytophthora Pseudoplusia Includens Spodoptera Eridania Lepidoptera Rachiplusia Spodoptera albula Plant pests Soybeans Brazil Spodoptera cosmioides |
topic |
Lepidotero Soja Praga de planta Phytophthora Pseudoplusia Includens Spodoptera Eridania Lepidoptera Rachiplusia Spodoptera albula Plant pests Soybeans Brazil Spodoptera cosmioides |
description |
The most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania, and S. albula) and R. nu from C. includens. However, Bpm I did not digest the amplicons from S. eridania and C. includens, which were discriminated by RFLP patterns produced by the restriction enzyme MboI. PCR-RFLP can be performed in a short period, and it is useful to distinguish the most important Brazilian Lepidoptera soybean pests. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-16T22:30:00Z 2013-12-16T22:30:00Z 2013-12-16 2013 2013-12-16T22:30:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Annals of the Entomological Society of America, v. 106, n. 5, p. 643-651, 2013. http://www.alice.cnptia.embrapa.br/alice/handle/doc/973974 10.1603/AN13049 |
identifier_str_mv |
Annals of the Entomological Society of America, v. 106, n. 5, p. 643-651, 2013. 10.1603/AN13049 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/973974 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1822720992008470528 |