Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220 |
Resumo: | Napier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification. |
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spelling |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars.Breeding programSSRElephant grassCapim ElefanteGramaNapier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification.Nota científica.ANA LUISA SOUSA AZEVEDO, CNPGL; FLÁVIA RANGEL DE SOUZA; ROSIANA ANGÉLICA CAMPOS; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; JUAREZ CAMPOLINA MACHADO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; FRANCISCO JOSE DA SILVA LEDO, CNPGL; MARCIO RESENDE, University of Florida.AZEVEDO, A. L. S.SOUZA, F. R. deCAMPOS, R. A.REIS, D. R. de L.MACHADO, J. C.MACHADO, M. A.LEDO, F. J. da S.RESENDE, M.2022-11-11T18:16:24Z2022-11-11T18:16:24Z2022-11-112022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, v. 22, n. 4, e42522244, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-11-11T18:16:24Zoai:www.alice.cnptia.embrapa.br:doc/1148220Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-11-11T18:16:24falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-11-11T18:16:24Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
title |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
spellingShingle |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. AZEVEDO, A. L. S. Breeding program SSR Elephant grass Capim Elefante Grama |
title_short |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
title_full |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
title_fullStr |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
title_full_unstemmed |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
title_sort |
Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars. |
author |
AZEVEDO, A. L. S. |
author_facet |
AZEVEDO, A. L. S. SOUZA, F. R. de CAMPOS, R. A. REIS, D. R. de L. MACHADO, J. C. MACHADO, M. A. LEDO, F. J. da S. RESENDE, M. |
author_role |
author |
author2 |
SOUZA, F. R. de CAMPOS, R. A. REIS, D. R. de L. MACHADO, J. C. MACHADO, M. A. LEDO, F. J. da S. RESENDE, M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
ANA LUISA SOUSA AZEVEDO, CNPGL; FLÁVIA RANGEL DE SOUZA; ROSIANA ANGÉLICA CAMPOS; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; JUAREZ CAMPOLINA MACHADO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; FRANCISCO JOSE DA SILVA LEDO, CNPGL; MARCIO RESENDE, University of Florida. |
dc.contributor.author.fl_str_mv |
AZEVEDO, A. L. S. SOUZA, F. R. de CAMPOS, R. A. REIS, D. R. de L. MACHADO, J. C. MACHADO, M. A. LEDO, F. J. da S. RESENDE, M. |
dc.subject.por.fl_str_mv |
Breeding program SSR Elephant grass Capim Elefante Grama |
topic |
Breeding program SSR Elephant grass Capim Elefante Grama |
description |
Napier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-11T18:16:24Z 2022-11-11T18:16:24Z 2022-11-11 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Crop Breeding and Applied Biotechnology, v. 22, n. 4, e42522244, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220 |
identifier_str_mv |
Crop Breeding and Applied Biotechnology, v. 22, n. 4, e42522244, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503534354366464 |