Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/903366 |
Resumo: | Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information. |
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Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.Genetic diversityMicrosatellite markerCoffeaDiscriminant analysisCoffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.Universidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; BioCafé; BioCafé; BioCafé; UFV; BioCafé/UFV.MISSIO, R. F.CAIXETA, E. T.ZAMBOLIM, E. M.PENA, G. F.ZAMBOLIM, L.DIAS, L. A. S.SAKIYAMA, N. S.2011-10-18T11:11:11Z2011-10-18T11:11:11Z2011-10-18T11:11:11Z2011-10-18T11:11:11Z2011-10-1820112011-10-18T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 10, n. 4, p 2366-2381, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/903366enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:34:55Zoai:www.alice.cnptia.embrapa.br:doc/903366Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:34:55falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:34:55Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
title |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
spellingShingle |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. MISSIO, R. F. Genetic diversity Microsatellite marker Coffea Discriminant analysis |
title_short |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
title_full |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
title_fullStr |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
title_full_unstemmed |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
title_sort |
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers. |
author |
MISSIO, R. F. |
author_facet |
MISSIO, R. F. CAIXETA, E. T. ZAMBOLIM, E. M. PENA, G. F. ZAMBOLIM, L. DIAS, L. A. S. SAKIYAMA, N. S. |
author_role |
author |
author2 |
CAIXETA, E. T. ZAMBOLIM, E. M. PENA, G. F. ZAMBOLIM, L. DIAS, L. A. S. SAKIYAMA, N. S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; BioCafé; BioCafé; BioCafé; UFV; BioCafé/UFV. |
dc.contributor.author.fl_str_mv |
MISSIO, R. F. CAIXETA, E. T. ZAMBOLIM, E. M. PENA, G. F. ZAMBOLIM, L. DIAS, L. A. S. SAKIYAMA, N. S. |
dc.subject.por.fl_str_mv |
Genetic diversity Microsatellite marker Coffea Discriminant analysis |
topic |
Genetic diversity Microsatellite marker Coffea Discriminant analysis |
description |
Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-18T11:11:11Z 2011-10-18T11:11:11Z 2011-10-18T11:11:11Z 2011-10-18T11:11:11Z 2011-10-18 2011 2011-10-18T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 10, n. 4, p 2366-2381, 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/903366 |
identifier_str_mv |
Genetics and Molecular Research, v. 10, n. 4, p 2366-2381, 2011. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/903366 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503350963666944 |