Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082755 |
Resumo: | ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. |
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Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.MicrobactériaBiodegradação de dioxanoABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas.HE, Y.MATHIEU, J.YANG, Y.YU, P.SILVA, M. L. B. daALVAREZ, P. J. J.2019-06-19T01:14:04Z2019-06-19T01:14:04Z2017-12-1820172019-06-19T01:14:04Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEnvironmental Science & Technology, v. 4, n. 11, p. 494-499, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/108275510.1021/acs.estlett.7b00456enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-06-19T01:14:11Zoai:www.alice.cnptia.embrapa.br:doc/1082755Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-06-19T01:14:11falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-06-19T01:14:11Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
title |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
spellingShingle |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. HE, Y. Microbactéria Biodegradação de dioxano |
title_short |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
title_full |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
title_fullStr |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
title_full_unstemmed |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
title_sort |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
author |
HE, Y. |
author_facet |
HE, Y. MATHIEU, J. YANG, Y. YU, P. SILVA, M. L. B. da ALVAREZ, P. J. J. |
author_role |
author |
author2 |
MATHIEU, J. YANG, Y. YU, P. SILVA, M. L. B. da ALVAREZ, P. J. J. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. |
dc.contributor.author.fl_str_mv |
HE, Y. MATHIEU, J. YANG, Y. YU, P. SILVA, M. L. B. da ALVAREZ, P. J. J. |
dc.subject.por.fl_str_mv |
Microbactéria Biodegradação de dioxano |
topic |
Microbactéria Biodegradação de dioxano |
description |
ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-18 2017 2019-06-19T01:14:04Z 2019-06-19T01:14:04Z 2019-06-19T01:14:04Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082755 10.1021/acs.estlett.7b00456 |
identifier_str_mv |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. 10.1021/acs.estlett.7b00456 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082755 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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cg-riaa@embrapa.br |
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