Maize endophytic bacteria as mineral phosphate solubilizers.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065208 |
Resumo: | In the present study, we demonstrated the in vitro activity of endophytic phosphate-solubilizing bacteria (PSB). Fifty-five endophytic PSB that were isolated from sap, leaves, and roots of maize were tested for their ability to solubilize tricalcium phosphate and produce organic acid. Partial sequencing of the 16S rRNA-encoding gene showed that the isolates were from the genus Bacillus and different species of Enterobacteriaceae. The phosphate solubilization index on solid medium and phosphate solubilization in liquid medium varied significantly among the isolates. There was a statistically significant difference (P ≤ 0.05) for both, the values of phosphate-solubilizing activity and pH of the growth medium, among the isolates. Pearson correlation was statistically significant (P ≤ 0.05) between P-solubilization and pH (R = -0.38), and between the gluconic acid production and the lowering of the pH of the liquid medium at 6 (R = 0.28) and 9 days (R = 0.39). Gluconic acid production was prevalent in all the PSB studied, and Bacillus species were most efficient in solubilizing phosphate. This is the first report on the characterization of bacterial endophytes from maize and their use as potential biofertilizers. In addition, this may provide an alternative strategy for improving the phosphorus acquisition efficiency of crop plants in tropical soils. |
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Maize endophytic bacteria as mineral phosphate solubilizers.Bactéria endofíticaBiócido solubilizanteFosfatoZea maysÁcido OrgânicoIn the present study, we demonstrated the in vitro activity of endophytic phosphate-solubilizing bacteria (PSB). Fifty-five endophytic PSB that were isolated from sap, leaves, and roots of maize were tested for their ability to solubilize tricalcium phosphate and produce organic acid. Partial sequencing of the 16S rRNA-encoding gene showed that the isolates were from the genus Bacillus and different species of Enterobacteriaceae. The phosphate solubilization index on solid medium and phosphate solubilization in liquid medium varied significantly among the isolates. There was a statistically significant difference (P ≤ 0.05) for both, the values of phosphate-solubilizing activity and pH of the growth medium, among the isolates. Pearson correlation was statistically significant (P ≤ 0.05) between P-solubilization and pH (R = -0.38), and between the gluconic acid production and the lowering of the pH of the liquid medium at 6 (R = 0.28) and 9 days (R = 0.39). Gluconic acid production was prevalent in all the PSB studied, and Bacillus species were most efficient in solubilizing phosphate. This is the first report on the characterization of bacterial endophytes from maize and their use as potential biofertilizers. In addition, this may provide an alternative strategy for improving the phosphorus acquisition efficiency of crop plants in tropical soils.Universidade Federal de São João Del ReiJOSE EDSON FONTES FIGUEIREDO, CNPMSCHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMSUniversidade Federal de Minas GeraisELIANE APARECIDA GOMES, CNPMSUniversidade Federal de São João Del ReiBEATRIZ DE ALMEIDA BARROS, CNPMSUBIRACI GOMES DE PAULA LANA, CNPMSIVANILDO EVODIO MARRIEL, CNPMS.ABREU, C. S. deFIGUEIREDO, J. E. F.OLIVEIRA-PAIVA, C. A.SANTOS, V. L. dosGOMES, E. A.RIBEIRO, V. P.BARROS, B. de A.LANA, U. G. de P.MARRIEL, I. E.2017-02-22T11:11:11Z2017-02-22T11:11:11Z2017-02-2220172017-11-21T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, v. 16, n. 1, p. 1-13, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/106520810.4238/gmr16019294enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:13:56Zoai:www.alice.cnptia.embrapa.br:doc/1065208Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:13:56falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:13:56Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Maize endophytic bacteria as mineral phosphate solubilizers. |
title |
Maize endophytic bacteria as mineral phosphate solubilizers. |
spellingShingle |
Maize endophytic bacteria as mineral phosphate solubilizers. ABREU, C. S. de Bactéria endofítica Biócido solubilizante Fosfato Zea mays Ácido Orgânico |
title_short |
Maize endophytic bacteria as mineral phosphate solubilizers. |
title_full |
Maize endophytic bacteria as mineral phosphate solubilizers. |
title_fullStr |
Maize endophytic bacteria as mineral phosphate solubilizers. |
title_full_unstemmed |
Maize endophytic bacteria as mineral phosphate solubilizers. |
title_sort |
Maize endophytic bacteria as mineral phosphate solubilizers. |
author |
ABREU, C. S. de |
author_facet |
ABREU, C. S. de FIGUEIREDO, J. E. F. OLIVEIRA-PAIVA, C. A. SANTOS, V. L. dos GOMES, E. A. RIBEIRO, V. P. BARROS, B. de A. LANA, U. G. de P. MARRIEL, I. E. |
author_role |
author |
author2 |
FIGUEIREDO, J. E. F. OLIVEIRA-PAIVA, C. A. SANTOS, V. L. dos GOMES, E. A. RIBEIRO, V. P. BARROS, B. de A. LANA, U. G. de P. MARRIEL, I. E. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São João Del Rei JOSE EDSON FONTES FIGUEIREDO, CNPMS CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS Universidade Federal de Minas Gerais ELIANE APARECIDA GOMES, CNPMS Universidade Federal de São João Del Rei BEATRIZ DE ALMEIDA BARROS, CNPMS UBIRACI GOMES DE PAULA LANA, CNPMS IVANILDO EVODIO MARRIEL, CNPMS. |
dc.contributor.author.fl_str_mv |
ABREU, C. S. de FIGUEIREDO, J. E. F. OLIVEIRA-PAIVA, C. A. SANTOS, V. L. dos GOMES, E. A. RIBEIRO, V. P. BARROS, B. de A. LANA, U. G. de P. MARRIEL, I. E. |
dc.subject.por.fl_str_mv |
Bactéria endofítica Biócido solubilizante Fosfato Zea mays Ácido Orgânico |
topic |
Bactéria endofítica Biócido solubilizante Fosfato Zea mays Ácido Orgânico |
description |
In the present study, we demonstrated the in vitro activity of endophytic phosphate-solubilizing bacteria (PSB). Fifty-five endophytic PSB that were isolated from sap, leaves, and roots of maize were tested for their ability to solubilize tricalcium phosphate and produce organic acid. Partial sequencing of the 16S rRNA-encoding gene showed that the isolates were from the genus Bacillus and different species of Enterobacteriaceae. The phosphate solubilization index on solid medium and phosphate solubilization in liquid medium varied significantly among the isolates. There was a statistically significant difference (P ≤ 0.05) for both, the values of phosphate-solubilizing activity and pH of the growth medium, among the isolates. Pearson correlation was statistically significant (P ≤ 0.05) between P-solubilization and pH (R = -0.38), and between the gluconic acid production and the lowering of the pH of the liquid medium at 6 (R = 0.28) and 9 days (R = 0.39). Gluconic acid production was prevalent in all the PSB studied, and Bacillus species were most efficient in solubilizing phosphate. This is the first report on the characterization of bacterial endophytes from maize and their use as potential biofertilizers. In addition, this may provide an alternative strategy for improving the phosphorus acquisition efficiency of crop plants in tropical soils. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-22T11:11:11Z 2017-02-22T11:11:11Z 2017-02-22 2017 2017-11-21T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 1, p. 1-13, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065208 10.4238/gmr16019294 |
identifier_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 1, p. 1-13, 2017. 10.4238/gmr16019294 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065208 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503432940290048 |