Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.

Detalhes bibliográficos
Autor(a) principal: CASSIOLATO, A. M. R.
Data de Publicação: 1999
Outros Autores: MELO, I. S. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152
Resumo: The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.
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spelling Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.TechniquesCulture mediumbenomylbiological controlfungimutantsplant pathogensThe isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.CASSIOLATO, A. M. R.MELO, I. S. de2019-07-18T00:44:14Z2019-07-18T00:44:14Z1999-09-0819992019-12-12T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlep. 43-46Revista de Microbiologia, v.30, p.43-46, 1999.http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-07-18T00:44:20Zoai:www.alice.cnptia.embrapa.br:doc/13152Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-07-18T00:44:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-07-18T00:44:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
spellingShingle Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
CASSIOLATO, A. M. R.
Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
title_short Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_full Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_fullStr Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_full_unstemmed Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_sort Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
author CASSIOLATO, A. M. R.
author_facet CASSIOLATO, A. M. R.
MELO, I. S. de
author_role author
author2 MELO, I. S. de
author2_role author
dc.contributor.none.fl_str_mv EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.
dc.contributor.author.fl_str_mv CASSIOLATO, A. M. R.
MELO, I. S. de
dc.subject.por.fl_str_mv Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
topic Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
description The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.
publishDate 1999
dc.date.none.fl_str_mv 1999-09-08
1999
2019-07-18T00:44:14Z
2019-07-18T00:44:14Z
2019-12-12T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista de Microbiologia, v.30, p.43-46, 1999.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152
identifier_str_mv Revista de Microbiologia, v.30, p.43-46, 1999.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv p. 43-46
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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