RAPD genotyping of banana cultivars from Epagri germplasm bank

Detalhes bibliográficos
Autor(a) principal: da Silva, Cristiane Maria
Data de Publicação: 2020
Outros Autores: Paulino, Eliza Clarindo, Pereira, Adriana, Hinz, Robert Harri, Lichtemberg, Luiz Alberto, Tcacenco, Fernando Adami, Stadnik, Marciel João
Tipo de documento: Artigo
Idioma: por
Título da fonte: Agropecuária Catarinense (Online)
Texto Completo: https://publicacoes.epagri.sc.gov.br/rac/article/view/579
Resumo: - Banana (Musa spp) is one of the most important fruits worldwide, and although there is a large number of cultivars,only a few present desirable agronomic characteristics. In order to facilitate advancements in plant breeding, there is a need forunderstanding the genomic structure and the genetic relationships among the accesses in germplasm banks. In this work, theuse of RAPD in the study of the genetic variability of 15 cultivars of banana from a wide genomic background was validated. Atotal of 78 primers were used, which generated 855 polymorphic bands. Data on presence/absence of polymorphic bands weresubjected to cluster analysis. The results showed that RAPD markers were efficient in revealing the genetic diversity among thecultivars, as well as in separating the A and B genomic groups and the cultivars accordingly to their subgroups.
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spelling RAPD genotyping of banana cultivars from Epagri germplasm bankGenotipagem de cultivares de bananeira do banco de germoplasma da Epagri por RAPDbananavariabilidade genéticamelhoramentomarcadores- Banana (Musa spp) is one of the most important fruits worldwide, and although there is a large number of cultivars,only a few present desirable agronomic characteristics. In order to facilitate advancements in plant breeding, there is a need forunderstanding the genomic structure and the genetic relationships among the accesses in germplasm banks. In this work, theuse of RAPD in the study of the genetic variability of 15 cultivars of banana from a wide genomic background was validated. Atotal of 78 primers were used, which generated 855 polymorphic bands. Data on presence/absence of polymorphic bands weresubjected to cluster analysis. The results showed that RAPD markers were efficient in revealing the genetic diversity among thecultivars, as well as in separating the A and B genomic groups and the cultivars accordingly to their subgroups.A banana (Musa spp.) está entre as frutas mais consumidas do mundo. Embora exista um número expressivo de cultivares de bananeira, poucos apresentam importância comercial. Para avanços nos programas de melhoramento genétco dessa cultura, existe a necessidade de compreender a estrutura genômica e as relações genétcas existentes entre os acessosdepositados nos bancos de germoplasma. Assim, o objetvo deste trabalho foi levantar a variabilidade genétca existente entre 15 acessos do Banco de Germoplasma de Bananeira da Epagri por meio da técnica RAPD. Foram utilizados 78 iniciadores de PCR, os quais geraram um total de 855 bandas polimórfcas. Os dados de presença/ausência de bandas foram submetidosà análise de conglomerados. Os resultados mostraram que os marcadores RAPD foram eficazes em revelar a existência de diversidade genétca entre os cultivares analisados, bem como em separar os grupos genômicos A e B e os cultivares de acordo com seus respectivos subgrupos. Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri2020-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://publicacoes.epagri.sc.gov.br/rac/article/view/579Agropecuária Catarinense Journal; Vol. 27 No. 2 (2014); 94-98Agropecuária Catarinense; v. 27 n. 2 (2014); 94-982525-60760103-0779reponame:Agropecuária Catarinense (Online)instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)instacron:EPAGRIporhttps://publicacoes.epagri.sc.gov.br/rac/article/view/579/483Copyright (c) 2020 Revista Agropecuária Catarinenseinfo:eu-repo/semantics/openAccessda Silva, Cristiane MariaPaulino, Eliza ClarindoPereira, AdrianaHinz, Robert HarriLichtemberg, Luiz AlbertoTcacenco, Fernando AdamiStadnik, Marciel João2021-02-17T13:35:13Zoai:ojs.publicacoes.epagri.sc.gov.br:article/579Revistahttps://publicacoes.epagri.sc.gov.br/RAC/indexPUBhttps://publicacoes.epagri.sc.gov.br/index.php/RAC/oaieditoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br2525-60760103-0779opendoar:2021-02-17T13:35:13Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)false
dc.title.none.fl_str_mv RAPD genotyping of banana cultivars from Epagri germplasm bank
Genotipagem de cultivares de bananeira do banco de germoplasma da Epagri por RAPD
title RAPD genotyping of banana cultivars from Epagri germplasm bank
spellingShingle RAPD genotyping of banana cultivars from Epagri germplasm bank
da Silva, Cristiane Maria
banana
variabilidade genética
melhoramento
marcadores
title_short RAPD genotyping of banana cultivars from Epagri germplasm bank
title_full RAPD genotyping of banana cultivars from Epagri germplasm bank
title_fullStr RAPD genotyping of banana cultivars from Epagri germplasm bank
title_full_unstemmed RAPD genotyping of banana cultivars from Epagri germplasm bank
title_sort RAPD genotyping of banana cultivars from Epagri germplasm bank
author da Silva, Cristiane Maria
author_facet da Silva, Cristiane Maria
Paulino, Eliza Clarindo
Pereira, Adriana
Hinz, Robert Harri
Lichtemberg, Luiz Alberto
Tcacenco, Fernando Adami
Stadnik, Marciel João
author_role author
author2 Paulino, Eliza Clarindo
Pereira, Adriana
Hinz, Robert Harri
Lichtemberg, Luiz Alberto
Tcacenco, Fernando Adami
Stadnik, Marciel João
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv da Silva, Cristiane Maria
Paulino, Eliza Clarindo
Pereira, Adriana
Hinz, Robert Harri
Lichtemberg, Luiz Alberto
Tcacenco, Fernando Adami
Stadnik, Marciel João
dc.subject.por.fl_str_mv banana
variabilidade genética
melhoramento
marcadores
topic banana
variabilidade genética
melhoramento
marcadores
description - Banana (Musa spp) is one of the most important fruits worldwide, and although there is a large number of cultivars,only a few present desirable agronomic characteristics. In order to facilitate advancements in plant breeding, there is a need forunderstanding the genomic structure and the genetic relationships among the accesses in germplasm banks. In this work, theuse of RAPD in the study of the genetic variability of 15 cultivars of banana from a wide genomic background was validated. Atotal of 78 primers were used, which generated 855 polymorphic bands. Data on presence/absence of polymorphic bands weresubjected to cluster analysis. The results showed that RAPD markers were efficient in revealing the genetic diversity among thecultivars, as well as in separating the A and B genomic groups and the cultivars accordingly to their subgroups.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://publicacoes.epagri.sc.gov.br/rac/article/view/579
url https://publicacoes.epagri.sc.gov.br/rac/article/view/579
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://publicacoes.epagri.sc.gov.br/rac/article/view/579/483
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista Agropecuária Catarinense
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista Agropecuária Catarinense
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri
publisher.none.fl_str_mv Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri
dc.source.none.fl_str_mv Agropecuária Catarinense Journal; Vol. 27 No. 2 (2014); 94-98
Agropecuária Catarinense; v. 27 n. 2 (2014); 94-98
2525-6076
0103-0779
reponame:Agropecuária Catarinense (Online)
instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
instacron:EPAGRI
instname_str Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
instacron_str EPAGRI
institution EPAGRI
reponame_str Agropecuária Catarinense (Online)
collection Agropecuária Catarinense (Online)
repository.name.fl_str_mv Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
repository.mail.fl_str_mv editoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br
_version_ 1754917259076173824

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