Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems

Bibliographic Details
Main Author: Zaffari, Gilmar Roberto
Publication Date: 2020
Other Authors: Koller, Osvino Leonardo, Soprano, Eliséo, Medeiros, Dilnei Souza, Stuker, Henri
Format: Article
Language: por
Source: Agropecuária Catarinense (Online)
Download full: https://publicacoes.epagri.sc.gov.br/rac/article/view/602
Summary: In order to increase the effectiveness of in vitro production of virus-free citrus seedlings, we assessed the effect of composition of different culture media on the development of shoot Apex meristem. The meristem were extracted, disinfected and inoculated in MS medium added with different combinations and concentrations of growth regulators. The best genotypeculture medium interaction was considered when the meristem obtained probability of developing greater than 0.75. The media culture that promoted best response had in its composition the presence of at least two (ANA and GA3; BAP and GA3; BAP and ANA) or the three growth regulators at low concentrations. Callus formation from the meristem was observed in all cultivars. The highest survival rate of meristem occurred in culture media with 0.5mg/L of ANA and 0.25 and 0.50mg/L of GA3. Direct cultivation of citrus did not prove to be promising. However, a suitable signal in the culture medium was settled to induce the early development of the shoots and callus differentiation in meristematic cells.
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spelling Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristemsPropagação clonal de cultivares de Citrus sp. pelo cultivo direto de meristemas in vitroMicropropagaçãolimpeza clonalcitrosorganogênese diretaIn order to increase the effectiveness of in vitro production of virus-free citrus seedlings, we assessed the effect of composition of different culture media on the development of shoot Apex meristem. The meristem were extracted, disinfected and inoculated in MS medium added with different combinations and concentrations of growth regulators. The best genotypeculture medium interaction was considered when the meristem obtained probability of developing greater than 0.75. The media culture that promoted best response had in its composition the presence of at least two (ANA and GA3; BAP and GA3; BAP and ANA) or the three growth regulators at low concentrations. Callus formation from the meristem was observed in all cultivars. The highest survival rate of meristem occurred in culture media with 0.5mg/L of ANA and 0.25 and 0.50mg/L of GA3. Direct cultivation of citrus did not prove to be promising. However, a suitable signal in the culture medium was settled to induce the early development of the shoots and callus differentiation in meristematic cells.Com objetivo de aumentar a eficácia da produção in vitro de mudas de citros livres de vírus, avaliou-se o efeito da composição de diferentes meios de cultura sobre o desenvolvimento de meristemas de ápices caulinares. Os meristemas foram extraídos, desinfestados e inoculados em meio MS adicionado de diferentes combinações e concentrações de reguladores de crescimento. A melhor interação genótpo-meio de cultura foi considerada quando os meristemas obtveram probabilidade dedesenvolvimento maior que 0,75. Os meios de cultura que promoveram melhor resposta tinham em sua composição a presença de pelo menos dois (ANA e GA3; BAP e GA3; BAP e ANA) ou os três reguladores de crescimento em baixas concentrações. A formação de calos a partr dos meristemas foi observada em todos os cultvares. A maior taxa de sobrevivência de meristemas ocorreu nos meios de cultura com 0,5mg/L de ANA e 0,25 e 0,50mg/L de GA3. O cultivo direto de meristemas de citros não mostrou ser promissor. Entretanto, estabeleceu-se uma sinalização adequada no meio de cultura para induzir o início dodesenvolvimento da parte aérea e a diferenciação de calos nas células meristemátcas. Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri2020-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://publicacoes.epagri.sc.gov.br/rac/article/view/602Agropecuária Catarinense Journal; Vol. 26 No. 1 (2013); 76-80Agropecuária Catarinense; v. 26 n. 1 (2013); 76-802525-60760103-0779reponame:Agropecuária Catarinense (Online)instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)instacron:EPAGRIporhttps://publicacoes.epagri.sc.gov.br/rac/article/view/602/504Copyright (c) 2020 Revista Agropecuária Catarinenseinfo:eu-repo/semantics/openAccessZaffari, Gilmar RobertoKoller, Osvino LeonardoSoprano, EliséoMedeiros, Dilnei SouzaStuker, Henri2021-02-17T13:27:31Zoai:ojs.publicacoes.epagri.sc.gov.br:article/602Revistahttps://publicacoes.epagri.sc.gov.br/RAC/indexPUBhttps://publicacoes.epagri.sc.gov.br/index.php/RAC/oaieditoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br2525-60760103-0779opendoar:2021-02-17T13:27:31Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)false
dc.title.none.fl_str_mv Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
Propagação clonal de cultivares de Citrus sp. pelo cultivo direto de meristemas in vitro
title Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
spellingShingle Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
Zaffari, Gilmar Roberto
Micropropagação
limpeza clonal
citros
organogênese direta
title_short Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
title_full Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
title_fullStr Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
title_full_unstemmed Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
title_sort Clonal propagation of Citrus sp. cultivars using direct cultivation of in vitro meristems
author Zaffari, Gilmar Roberto
author_facet Zaffari, Gilmar Roberto
Koller, Osvino Leonardo
Soprano, Eliséo
Medeiros, Dilnei Souza
Stuker, Henri
author_role author
author2 Koller, Osvino Leonardo
Soprano, Eliséo
Medeiros, Dilnei Souza
Stuker, Henri
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zaffari, Gilmar Roberto
Koller, Osvino Leonardo
Soprano, Eliséo
Medeiros, Dilnei Souza
Stuker, Henri
dc.subject.por.fl_str_mv Micropropagação
limpeza clonal
citros
organogênese direta
topic Micropropagação
limpeza clonal
citros
organogênese direta
description In order to increase the effectiveness of in vitro production of virus-free citrus seedlings, we assessed the effect of composition of different culture media on the development of shoot Apex meristem. The meristem were extracted, disinfected and inoculated in MS medium added with different combinations and concentrations of growth regulators. The best genotypeculture medium interaction was considered when the meristem obtained probability of developing greater than 0.75. The media culture that promoted best response had in its composition the presence of at least two (ANA and GA3; BAP and GA3; BAP and ANA) or the three growth regulators at low concentrations. Callus formation from the meristem was observed in all cultivars. The highest survival rate of meristem occurred in culture media with 0.5mg/L of ANA and 0.25 and 0.50mg/L of GA3. Direct cultivation of citrus did not prove to be promising. However, a suitable signal in the culture medium was settled to induce the early development of the shoots and callus differentiation in meristematic cells.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://publicacoes.epagri.sc.gov.br/rac/article/view/602
url https://publicacoes.epagri.sc.gov.br/rac/article/view/602
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://publicacoes.epagri.sc.gov.br/rac/article/view/602/504
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista Agropecuária Catarinense
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista Agropecuária Catarinense
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri
publisher.none.fl_str_mv Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri
dc.source.none.fl_str_mv Agropecuária Catarinense Journal; Vol. 26 No. 1 (2013); 76-80
Agropecuária Catarinense; v. 26 n. 1 (2013); 76-80
2525-6076
0103-0779
reponame:Agropecuária Catarinense (Online)
instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
instacron:EPAGRI
instname_str Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
instacron_str EPAGRI
institution EPAGRI
reponame_str Agropecuária Catarinense (Online)
collection Agropecuária Catarinense (Online)
repository.name.fl_str_mv Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
repository.mail.fl_str_mv editoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br
_version_ 1754917259110776832

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