Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation.
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Laticínios Cândido Tostes |
Texto Completo: | https://www.revistadoilct.com.br/rilct/article/view/67 |
Resumo: | In the last years studies have been made to find viable applications to the cheese whey due to the large volume produced daily and the environment pollution caused by the absence of an appropriate destination for it. Among many researches in biotechnological utilization of cheese whey came of its use as a substrate for fermentation aiming to obtain some products being one of those the b-galactosidase. This study had the objective to heat treat cheese whey to eliminate the contaminant microorganisms without modifying the stability of the protein structure. The cheese whey was heat treated with flowing steam for 3, 5, 10 and 15 minutes and with pressure for 3, 5, 10 and 15 minutes. After the thermal treatment a counting in plates was made in PCA growth medium and the protein precipitation was verified visually. The experimental results showed that the treatment under pressure is efficient to control de microbiological growth, but a huge protein precipitation happened. However the thermal treatment with flowing steam was efficient in the microorganism's elimination without visible protein denaturation. Thus, for the production of âgalactosidase we will use a thermal treatment with flowing steam. |
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Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation.Tratamento térmico do soro de queijo para determinação de condições ideais para produção de β-galactosidase por fermentação com kluyveromyces marxianus.b-galactosidase, protein denaturation, biotechnical use, Kluyveromyces marxianusβ-galactosidase; desnaturação protéica; aproveitamentoIn the last years studies have been made to find viable applications to the cheese whey due to the large volume produced daily and the environment pollution caused by the absence of an appropriate destination for it. Among many researches in biotechnological utilization of cheese whey came of its use as a substrate for fermentation aiming to obtain some products being one of those the b-galactosidase. This study had the objective to heat treat cheese whey to eliminate the contaminant microorganisms without modifying the stability of the protein structure. The cheese whey was heat treated with flowing steam for 3, 5, 10 and 15 minutes and with pressure for 3, 5, 10 and 15 minutes. After the thermal treatment a counting in plates was made in PCA growth medium and the protein precipitation was verified visually. The experimental results showed that the treatment under pressure is efficient to control de microbiological growth, but a huge protein precipitation happened. However the thermal treatment with flowing steam was efficient in the microorganism's elimination without visible protein denaturation. Thus, for the production of âgalactosidase we will use a thermal treatment with flowing steam. Nos últimos anos têm-se estudado aplicações viáveis para o soro do queijo, devido ao grande volume produzido diariamente e da poluição ambiental causada pela ausência de um destino adequado para o mesmo. Dentre as várias pesquisas de aproveitamento biotecnológico do soro de queijo, destaca-se a sua utilização como substrato para fermentação visando à obtenção de vários produtos, dentre eles a b-galactosidase. Este trabalho teve como objetivo tratar o soro de queijo termicamente para extinguir a microbiota contaminante sem alterar a estabilidade da estrutura protéica. O soro de queijo foi tratado termicamente por vapor fluente durante 3, 5, 10 e 15 minutos e por pressão durante 3, 5, 10 e 15 minutos. Após o tratamento térmico, foi feita uma contagem em placas em meio de cultura PCA e a precipitação das proteínas foi verificada visualmente. Os resultados experimentais mostraram que o tratamento sob pressão é eficaz para inviabilizar o crescimento microbiano, entretanto ocorre uma desnaturação protéica bem acentuada. Já o tratamento térmico por vapor fluente foi eficaz na eliminação dos microrganismos e não ocorreu a desnaturação protéica visível. Assim, para a produção de b-galactosidase será empregado o tratamento térmico sob vapor fluente. desnaturação protéica; aproveitamentoILCTHott, SaraAmaral, Lorena MachadoCastro-Gómez, Raúl J. H.Bravo-Martins, Claudia E. C.2013-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistadoilct.com.br/rilct/article/view/67Journal of Candido Tostes Dairy Institute; v. 63, n. 365 (2008); 32-35Revista do Instituto de Laticínios Cândido Tostes; v. 63, n. 365 (2008); 32-352238-64160100-3674reponame:Revista do Instituto de Laticínios Cândido Tostesinstname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)instacron:EPAMIGporhttps://www.revistadoilct.com.br/rilct/article/view/67/73Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostesinfo:eu-repo/semantics/openAccess2013-12-19T16:24:53Zoai:oai.rilct.emnuvens.com.br:article/67Revistahttp://www.revistadoilct.com.br/ONGhttps://www.revistadoilct.com.br/rilct/oai||revistadoilct@epamig.br|| revistadoilct@oi.com.br2238-64160100-3674opendoar:2013-12-19T16:24:53Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)false |
dc.title.none.fl_str_mv |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. Tratamento térmico do soro de queijo para determinação de condições ideais para produção de β-galactosidase por fermentação com kluyveromyces marxianus. |
title |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. |
spellingShingle |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. Hott, Sara b-galactosidase, protein denaturation, biotechnical use, Kluyveromyces marxianus β-galactosidase; desnaturação protéica; aproveitamento |
title_short |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. |
title_full |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. |
title_fullStr |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. |
title_full_unstemmed |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. |
title_sort |
Thermal treatment of cheese whey for determination of suitable conditions for β- galactosidase production by Kluyveromyces marxianus fermentation. |
author |
Hott, Sara |
author_facet |
Hott, Sara Amaral, Lorena Machado Castro-Gómez, Raúl J. H. Bravo-Martins, Claudia E. C. |
author_role |
author |
author2 |
Amaral, Lorena Machado Castro-Gómez, Raúl J. H. Bravo-Martins, Claudia E. C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Hott, Sara Amaral, Lorena Machado Castro-Gómez, Raúl J. H. Bravo-Martins, Claudia E. C. |
dc.subject.none.fl_str_mv |
|
dc.subject.por.fl_str_mv |
b-galactosidase, protein denaturation, biotechnical use, Kluyveromyces marxianus β-galactosidase; desnaturação protéica; aproveitamento |
topic |
b-galactosidase, protein denaturation, biotechnical use, Kluyveromyces marxianus β-galactosidase; desnaturação protéica; aproveitamento |
description |
In the last years studies have been made to find viable applications to the cheese whey due to the large volume produced daily and the environment pollution caused by the absence of an appropriate destination for it. Among many researches in biotechnological utilization of cheese whey came of its use as a substrate for fermentation aiming to obtain some products being one of those the b-galactosidase. This study had the objective to heat treat cheese whey to eliminate the contaminant microorganisms without modifying the stability of the protein structure. The cheese whey was heat treated with flowing steam for 3, 5, 10 and 15 minutes and with pressure for 3, 5, 10 and 15 minutes. After the thermal treatment a counting in plates was made in PCA growth medium and the protein precipitation was verified visually. The experimental results showed that the treatment under pressure is efficient to control de microbiological growth, but a huge protein precipitation happened. However the thermal treatment with flowing steam was efficient in the microorganism's elimination without visible protein denaturation. Thus, for the production of âgalactosidase we will use a thermal treatment with flowing steam. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-19 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistadoilct.com.br/rilct/article/view/67 |
url |
https://www.revistadoilct.com.br/rilct/article/view/67 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistadoilct.com.br/rilct/article/view/67/73 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostes info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostes |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ILCT |
publisher.none.fl_str_mv |
ILCT |
dc.source.none.fl_str_mv |
Journal of Candido Tostes Dairy Institute; v. 63, n. 365 (2008); 32-35 Revista do Instituto de Laticínios Cândido Tostes; v. 63, n. 365 (2008); 32-35 2238-6416 0100-3674 reponame:Revista do Instituto de Laticínios Cândido Tostes instname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) instacron:EPAMIG |
instname_str |
Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
instacron_str |
EPAMIG |
institution |
EPAMIG |
reponame_str |
Revista do Instituto de Laticínios Cândido Tostes |
collection |
Revista do Instituto de Laticínios Cândido Tostes |
repository.name.fl_str_mv |
Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
repository.mail.fl_str_mv |
||revistadoilct@epamig.br|| revistadoilct@oi.com.br |
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1793890270041866240 |