Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds

Detalhes bibliográficos
Autor(a) principal: Zhang,B
Data de Publicação: 2019
Outros Autores: Xiang,D, Yang,R, Yang,L, Li,J, Zhao,Z
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100318
Resumo: ABSTRACT Adipose differentiation-related protein (ADFP) is a fatty acid-binding protein that can promote the absorption of long-chain fatty acids. However, few results have been published regarding its role in Yunnan Native chicken breeds. The aim of this study was to determine ADFP gene tissue-specific expression in Piao chickens (PC) and Wuliangshan black-bone chickens (WBC) by RT-qPCR. The ontogenetic expression levels of the ADFP gene were significantly different during growth and development phases in the subcutaneous fat, liver, and pectoralis muscle of PC, and in the subcutaneous fat, liver, and pectoralis muscle of WBC (p<0.05). Individual tissue-differential expression levelswere detectedon d 91 and 112 in PC, with highest levels determined in abdominal fat and subcutaneous fat, respectively. However, in WBC, the highest levels were determined on d 49, 91 and 112 d in the pectoralis muscle and liver. Correlation analysis revealed ADFP expression level in liver of WBC was significantly related with LW and HC (p<0.05), while no significant correlations with carcass fatness (CF) were found in PC (p>0.05). The results suggest ADFPdifferential expression in the liver and pectoral muscles of PC and WBC during the growth and development phases (p<0.05). The observed expression patterns indicate that the ADFP gene plays an important role in lipid metabolism of PC and WBC, and that these patterns are expressed differently in the tissues of different chicken genotypes.
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spelling Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken BreedsADFPcarcass fatnessPiao chickensRT-qPCRWuliangshan black-bone chickensABSTRACT Adipose differentiation-related protein (ADFP) is a fatty acid-binding protein that can promote the absorption of long-chain fatty acids. However, few results have been published regarding its role in Yunnan Native chicken breeds. The aim of this study was to determine ADFP gene tissue-specific expression in Piao chickens (PC) and Wuliangshan black-bone chickens (WBC) by RT-qPCR. The ontogenetic expression levels of the ADFP gene were significantly different during growth and development phases in the subcutaneous fat, liver, and pectoralis muscle of PC, and in the subcutaneous fat, liver, and pectoralis muscle of WBC (p<0.05). Individual tissue-differential expression levelswere detectedon d 91 and 112 in PC, with highest levels determined in abdominal fat and subcutaneous fat, respectively. However, in WBC, the highest levels were determined on d 49, 91 and 112 d in the pectoralis muscle and liver. Correlation analysis revealed ADFP expression level in liver of WBC was significantly related with LW and HC (p<0.05), while no significant correlations with carcass fatness (CF) were found in PC (p>0.05). The results suggest ADFPdifferential expression in the liver and pectoral muscles of PC and WBC during the growth and development phases (p<0.05). The observed expression patterns indicate that the ADFP gene plays an important role in lipid metabolism of PC and WBC, and that these patterns are expressed differently in the tissues of different chicken genotypes.Fundacao de Apoio a Ciência e Tecnologia Avicolas2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100318Brazilian Journal of Poultry Science v.21 n.1 2019reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1806-9061-2017-0780info:eu-repo/semantics/openAccessZhang,BXiang,DYang,RYang,LLi,JZhao,Zeng2019-05-06T00:00:00Zoai:scielo:S1516-635X2019000100318Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2019-05-06T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
title Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
spellingShingle Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
Zhang,B
ADFP
carcass fatness
Piao chickens
RT-qPCR
Wuliangshan black-bone chickens
title_short Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
title_full Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
title_fullStr Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
title_full_unstemmed Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
title_sort Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
author Zhang,B
author_facet Zhang,B
Xiang,D
Yang,R
Yang,L
Li,J
Zhao,Z
author_role author
author2 Xiang,D
Yang,R
Yang,L
Li,J
Zhao,Z
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Zhang,B
Xiang,D
Yang,R
Yang,L
Li,J
Zhao,Z
dc.subject.por.fl_str_mv ADFP
carcass fatness
Piao chickens
RT-qPCR
Wuliangshan black-bone chickens
topic ADFP
carcass fatness
Piao chickens
RT-qPCR
Wuliangshan black-bone chickens
description ABSTRACT Adipose differentiation-related protein (ADFP) is a fatty acid-binding protein that can promote the absorption of long-chain fatty acids. However, few results have been published regarding its role in Yunnan Native chicken breeds. The aim of this study was to determine ADFP gene tissue-specific expression in Piao chickens (PC) and Wuliangshan black-bone chickens (WBC) by RT-qPCR. The ontogenetic expression levels of the ADFP gene were significantly different during growth and development phases in the subcutaneous fat, liver, and pectoralis muscle of PC, and in the subcutaneous fat, liver, and pectoralis muscle of WBC (p<0.05). Individual tissue-differential expression levelswere detectedon d 91 and 112 in PC, with highest levels determined in abdominal fat and subcutaneous fat, respectively. However, in WBC, the highest levels were determined on d 49, 91 and 112 d in the pectoralis muscle and liver. Correlation analysis revealed ADFP expression level in liver of WBC was significantly related with LW and HC (p<0.05), while no significant correlations with carcass fatness (CF) were found in PC (p>0.05). The results suggest ADFPdifferential expression in the liver and pectoral muscles of PC and WBC during the growth and development phases (p<0.05). The observed expression patterns indicate that the ADFP gene plays an important role in lipid metabolism of PC and WBC, and that these patterns are expressed differently in the tissues of different chicken genotypes.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100318
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100318
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9061-2017-0780
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.21 n.1 2019
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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