In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Poultry Science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012 |
Resumo: | The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes. |
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Brazilian Journal of Poultry Science (Online) |
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In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickensAflatoxin B1BroilersLymphocytesPicoGreenReactive oxygen speciesThe aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.Fundacao de Apoio a Ciência e Tecnologia Avicolas2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012Brazilian Journal of Poultry Science v.16 n.3 2014reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1516-635x1603307-312info:eu-repo/semantics/openAccessZimmermann,CEPMachado,AKCadoná,FCJaques,JASSchlemmer,KBLautert,CCruz,IBMZanette,RALeal,DBRSanturio,JMeng2014-10-01T00:00:00Zoai:scielo:S1516-635X2014000300012Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2014-10-01T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false |
dc.title.none.fl_str_mv |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
title |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
spellingShingle |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens Zimmermann,CEP Aflatoxin B1 Broilers Lymphocytes PicoGreen Reactive oxygen species |
title_short |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
title_full |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
title_fullStr |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
title_full_unstemmed |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
title_sort |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
author |
Zimmermann,CEP |
author_facet |
Zimmermann,CEP Machado,AK Cadoná,FC Jaques,JAS Schlemmer,KB Lautert,C Cruz,IBM Zanette,RA Leal,DBR Santurio,JM |
author_role |
author |
author2 |
Machado,AK Cadoná,FC Jaques,JAS Schlemmer,KB Lautert,C Cruz,IBM Zanette,RA Leal,DBR Santurio,JM |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zimmermann,CEP Machado,AK Cadoná,FC Jaques,JAS Schlemmer,KB Lautert,C Cruz,IBM Zanette,RA Leal,DBR Santurio,JM |
dc.subject.por.fl_str_mv |
Aflatoxin B1 Broilers Lymphocytes PicoGreen Reactive oxygen species |
topic |
Aflatoxin B1 Broilers Lymphocytes PicoGreen Reactive oxygen species |
description |
The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1516-635x1603307-312 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Brazilian Journal of Poultry Science v.16 n.3 2014 reponame:Brazilian Journal of Poultry Science (Online) instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
instname_str |
Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
instacron_str |
FACTA |
institution |
FACTA |
reponame_str |
Brazilian Journal of Poultry Science (Online) |
collection |
Brazilian Journal of Poultry Science (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
repository.mail.fl_str_mv |
||rvfacta@terra.com.br |
_version_ |
1754122512650731520 |