Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil

Detalhes bibliográficos
Autor(a) principal: Coswig,LT
Data de Publicação: 2007
Outros Autores: Stach-Machado,DR, Arns,CW
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400008
Resumo: Avian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91) of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.
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spelling Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in BrazilAvian metapneumovirusBrazilmonoclonal antibodiesneutralizing epitopesAvian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91) of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.Fundacao de Apoio a Ciência e Tecnologia Avicolas2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400008Brazilian Journal of Poultry Science v.9 n.4 2007reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/S1516-635X2007000400008info:eu-repo/semantics/openAccessCoswig,LTStach-Machado,DRArns,CWeng2008-03-12T00:00:00Zoai:scielo:S1516-635X2007000400008Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2008-03-12T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
title Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
spellingShingle Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
Coswig,LT
Avian metapneumovirus
Brazil
monoclonal antibodies
neutralizing epitopes
title_short Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
title_full Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
title_fullStr Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
title_full_unstemmed Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
title_sort Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil
author Coswig,LT
author_facet Coswig,LT
Stach-Machado,DR
Arns,CW
author_role author
author2 Stach-Machado,DR
Arns,CW
author2_role author
author
dc.contributor.author.fl_str_mv Coswig,LT
Stach-Machado,DR
Arns,CW
dc.subject.por.fl_str_mv Avian metapneumovirus
Brazil
monoclonal antibodies
neutralizing epitopes
topic Avian metapneumovirus
Brazil
monoclonal antibodies
neutralizing epitopes
description Avian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91) of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-635X2007000400008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.9 n.4 2007
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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