The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Poultry Science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400314 |
Resumo: | ABSTRACT Chicken coccidiosis is a parasitic disease caused by one or more species of Eimeria infection with severe consequences. The NF-κB signaling pathway and TGF-β4 play an important role in the inflammation and immune response caused by coccidiosis infection. This study was designed to investigate the changes of NF-κB signaling pathway and the effects of TGF-β4 silencing on the expression of NF-κB signaling pathway in chicken’s intestinal epithelial cells (IECs) after infecting with E. tenella sporozoites. TGF-β4 small interfering RNA (TGF-β4 siRNA) sequences were transfected into chicken IECs for reducing TGF-β4 expression. The results showed that compared with uninfected control group (Con.), the proinflammatory factors of NF-κB, IL-6, IL-2, IL-1β increased significantly in the E. tenella infected group (ET) (p<0.05). In comparison with the IECs in the ET, the expression level of NF-κB, IL-6, IL-2, IL-1β dropped significantly in the group infected both by E. tenella and TGF-β4 siRNA vector (ET+siRNA) (p<0.05). The results of this experiment indicate that NF-κB signaling pathway is closely correlated with TGF-β4 in IECs infected with coccidia sporozoites. TGF-β4 plays an important role in regulating the immune function of coccidia-infected chicken epithelial cells through NF-κB signaling pathway and preventing immune-mediated pathological changes. |
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Brazilian Journal of Poultry Science (Online) |
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The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenellaNF-κB signaling pathwayTGF-β4IECsCoccidiosisChickenABSTRACT Chicken coccidiosis is a parasitic disease caused by one or more species of Eimeria infection with severe consequences. The NF-κB signaling pathway and TGF-β4 play an important role in the inflammation and immune response caused by coccidiosis infection. This study was designed to investigate the changes of NF-κB signaling pathway and the effects of TGF-β4 silencing on the expression of NF-κB signaling pathway in chicken’s intestinal epithelial cells (IECs) after infecting with E. tenella sporozoites. TGF-β4 small interfering RNA (TGF-β4 siRNA) sequences were transfected into chicken IECs for reducing TGF-β4 expression. The results showed that compared with uninfected control group (Con.), the proinflammatory factors of NF-κB, IL-6, IL-2, IL-1β increased significantly in the E. tenella infected group (ET) (p<0.05). In comparison with the IECs in the ET, the expression level of NF-κB, IL-6, IL-2, IL-1β dropped significantly in the group infected both by E. tenella and TGF-β4 siRNA vector (ET+siRNA) (p<0.05). The results of this experiment indicate that NF-κB signaling pathway is closely correlated with TGF-β4 in IECs infected with coccidia sporozoites. TGF-β4 plays an important role in regulating the immune function of coccidia-infected chicken epithelial cells through NF-κB signaling pathway and preventing immune-mediated pathological changes.Fundacao de Apoio a Ciência e Tecnologia Avicolas2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400314Brazilian Journal of Poultry Science v.22 n.4 2020reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1806-9061-2020-1338info:eu-repo/semantics/openAccessJin,HHaicheng,YCaiyun,ZYong,ZJinrong,Weng2020-12-10T00:00:00Zoai:scielo:S1516-635X2020000400314Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2020-12-10T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false |
dc.title.none.fl_str_mv |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
title |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
spellingShingle |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella Jin,H NF-κB signaling pathway TGF-β4 IECs Coccidiosis Chicken |
title_short |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
title_full |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
title_fullStr |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
title_full_unstemmed |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
title_sort |
The Expression of NF-kB Signaling Pathway Was Inhibited by Silencing TGF-b4 in Chicken IECs Infected with E. tenella |
author |
Jin,H |
author_facet |
Jin,H Haicheng,Y Caiyun,Z Yong,Z Jinrong,W |
author_role |
author |
author2 |
Haicheng,Y Caiyun,Z Yong,Z Jinrong,W |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Jin,H Haicheng,Y Caiyun,Z Yong,Z Jinrong,W |
dc.subject.por.fl_str_mv |
NF-κB signaling pathway TGF-β4 IECs Coccidiosis Chicken |
topic |
NF-κB signaling pathway TGF-β4 IECs Coccidiosis Chicken |
description |
ABSTRACT Chicken coccidiosis is a parasitic disease caused by one or more species of Eimeria infection with severe consequences. The NF-κB signaling pathway and TGF-β4 play an important role in the inflammation and immune response caused by coccidiosis infection. This study was designed to investigate the changes of NF-κB signaling pathway and the effects of TGF-β4 silencing on the expression of NF-κB signaling pathway in chicken’s intestinal epithelial cells (IECs) after infecting with E. tenella sporozoites. TGF-β4 small interfering RNA (TGF-β4 siRNA) sequences were transfected into chicken IECs for reducing TGF-β4 expression. The results showed that compared with uninfected control group (Con.), the proinflammatory factors of NF-κB, IL-6, IL-2, IL-1β increased significantly in the E. tenella infected group (ET) (p<0.05). In comparison with the IECs in the ET, the expression level of NF-κB, IL-6, IL-2, IL-1β dropped significantly in the group infected both by E. tenella and TGF-β4 siRNA vector (ET+siRNA) (p<0.05). The results of this experiment indicate that NF-κB signaling pathway is closely correlated with TGF-β4 in IECs infected with coccidia sporozoites. TGF-β4 plays an important role in regulating the immune function of coccidia-infected chicken epithelial cells through NF-κB signaling pathway and preventing immune-mediated pathological changes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400314 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400314 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-9061-2020-1338 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Brazilian Journal of Poultry Science v.22 n.4 2020 reponame:Brazilian Journal of Poultry Science (Online) instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
instname_str |
Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
instacron_str |
FACTA |
institution |
FACTA |
reponame_str |
Brazilian Journal of Poultry Science (Online) |
collection |
Brazilian Journal of Poultry Science (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
repository.mail.fl_str_mv |
||rvfacta@terra.com.br |
_version_ |
1754122515622395904 |