Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis

Detalhes bibliográficos
Autor(a) principal: MRFB,Martins
Data de Publicação: 2016
Outros Autores: Mendes,AA, Milbradt,EL, Almeida Paz,ICL, Martins,BB, Fernandes,BCS
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000500057
Resumo: ABSTRACT The objective of this study was evaluated the villus integrity of commercial turkeys submitted to coccidiosis prevention methods and challenged with Eimeria field oocysts, using scanning electron microscopy. Sixty BUT 9 female commercial turkeys were distributed in a completely randomized block design split with two treatments: T1- control diet without vaccinations against coccidiosis and anticoccidial drug, and T2- vaccinated against coccidiosis (commercial vaccine). On d 21 of life, all birds of all treatments were challenged with a mixed-species containing E. meleagrimitis and E. galopavonis, via crop intubation with 1 mL of ~20,000 sporulated oocysts/bird. The size of the inoculum was determined in previous experiments. At 27 and 70 days of age, five birds per treatment were randomly removed to evaluate the intestinal integrity. Duodenum, jejunum and ileum segments were collected and processed according to routine scanning electron microscope. The enteric mucosa integrity of the commercial turkeys subjected to coccidiosis vaccination was reduced when compared to the birds of the control treatment. There was no difference in the performance results, therefore these results cannot be attributed to the vaccination as well as to the ineffectiveness against the challenge that was administered.
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spelling Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against CoccidiosisBirddiseasespoultry productionprotozoan parasitesABSTRACT The objective of this study was evaluated the villus integrity of commercial turkeys submitted to coccidiosis prevention methods and challenged with Eimeria field oocysts, using scanning electron microscopy. Sixty BUT 9 female commercial turkeys were distributed in a completely randomized block design split with two treatments: T1- control diet without vaccinations against coccidiosis and anticoccidial drug, and T2- vaccinated against coccidiosis (commercial vaccine). On d 21 of life, all birds of all treatments were challenged with a mixed-species containing E. meleagrimitis and E. galopavonis, via crop intubation with 1 mL of ~20,000 sporulated oocysts/bird. The size of the inoculum was determined in previous experiments. At 27 and 70 days of age, five birds per treatment were randomly removed to evaluate the intestinal integrity. Duodenum, jejunum and ileum segments were collected and processed according to routine scanning electron microscope. The enteric mucosa integrity of the commercial turkeys subjected to coccidiosis vaccination was reduced when compared to the birds of the control treatment. There was no difference in the performance results, therefore these results cannot be attributed to the vaccination as well as to the ineffectiveness against the challenge that was administered.Fundacao de Apoio a Ciência e Tecnologia Avicolas2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000500057Brazilian Journal of Poultry Science v.18 n.spe 2016reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/11806-90615760info:eu-repo/semantics/openAccessMRFB,MartinsMendes,AAMilbradt,ELAlmeida Paz,ICLMartins,BBFernandes,BCSeng2016-08-31T00:00:00Zoai:scielo:S1516-635X2016000500057Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2016-08-31T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
title Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
spellingShingle Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
MRFB,Martins
Bird
diseases
poultry production
protozoan parasites
title_short Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
title_full Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
title_fullStr Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
title_full_unstemmed Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
title_sort Ultrastructural Assessment of the Integrity of the enteric Mucosa of Commercial Turkeys Vaccinated against Coccidiosis
author MRFB,Martins
author_facet MRFB,Martins
Mendes,AA
Milbradt,EL
Almeida Paz,ICL
Martins,BB
Fernandes,BCS
author_role author
author2 Mendes,AA
Milbradt,EL
Almeida Paz,ICL
Martins,BB
Fernandes,BCS
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv MRFB,Martins
Mendes,AA
Milbradt,EL
Almeida Paz,ICL
Martins,BB
Fernandes,BCS
dc.subject.por.fl_str_mv Bird
diseases
poultry production
protozoan parasites
topic Bird
diseases
poultry production
protozoan parasites
description ABSTRACT The objective of this study was evaluated the villus integrity of commercial turkeys submitted to coccidiosis prevention methods and challenged with Eimeria field oocysts, using scanning electron microscopy. Sixty BUT 9 female commercial turkeys were distributed in a completely randomized block design split with two treatments: T1- control diet without vaccinations against coccidiosis and anticoccidial drug, and T2- vaccinated against coccidiosis (commercial vaccine). On d 21 of life, all birds of all treatments were challenged with a mixed-species containing E. meleagrimitis and E. galopavonis, via crop intubation with 1 mL of ~20,000 sporulated oocysts/bird. The size of the inoculum was determined in previous experiments. At 27 and 70 days of age, five birds per treatment were randomly removed to evaluate the intestinal integrity. Duodenum, jejunum and ileum segments were collected and processed according to routine scanning electron microscope. The enteric mucosa integrity of the commercial turkeys subjected to coccidiosis vaccination was reduced when compared to the birds of the control treatment. There was no difference in the performance results, therefore these results cannot be attributed to the vaccination as well as to the ineffectiveness against the challenge that was administered.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000500057
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000500057
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/11806-90615760
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.18 n.spe 2016
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
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reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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