Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Poultry Science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055 |
Resumo: | ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle. |
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Brazilian Journal of Poultry Science (Online) |
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|
spelling |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast MuscleFeeding conditionschickenFATP1meat qualityintramuscular fatABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle.Fundacao de Apoio a Ciência e Tecnologia Avicolas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055Brazilian Journal of Poultry Science v.19 n.spe 2017reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1806-9061-2016-0367info:eu-repo/semantics/openAccessGao,GWang,HZhao,XLi,QWang,CLi,JZhang,KZhong,HWang,Qeng2017-05-18T00:00:00Zoai:scielo:S1516-635X2017000500055Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2017-05-18T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false |
dc.title.none.fl_str_mv |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
spellingShingle |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle Gao,G Feeding conditions chicken FATP1 meat quality intramuscular fat |
title_short |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_full |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_fullStr |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_full_unstemmed |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_sort |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
author |
Gao,G |
author_facet |
Gao,G Wang,H Zhao,X Li,Q Wang,C Li,J Zhang,K Zhong,H Wang,Q |
author_role |
author |
author2 |
Wang,H Zhao,X Li,Q Wang,C Li,J Zhang,K Zhong,H Wang,Q |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gao,G Wang,H Zhao,X Li,Q Wang,C Li,J Zhang,K Zhong,H Wang,Q |
dc.subject.por.fl_str_mv |
Feeding conditions chicken FATP1 meat quality intramuscular fat |
topic |
Feeding conditions chicken FATP1 meat quality intramuscular fat |
description |
ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-9061-2016-0367 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Brazilian Journal of Poultry Science v.19 n.spe 2017 reponame:Brazilian Journal of Poultry Science (Online) instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
instname_str |
Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
instacron_str |
FACTA |
institution |
FACTA |
reponame_str |
Brazilian Journal of Poultry Science (Online) |
collection |
Brazilian Journal of Poultry Science (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
repository.mail.fl_str_mv |
||rvfacta@terra.com.br |
_version_ |
1754122514274975744 |