Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle

Detalhes bibliográficos
Autor(a) principal: Gao,G
Data de Publicação: 2017
Outros Autores: Wang,H, Zhao,X, Li,Q, Wang,C, Li,J, Zhang,K, Zhong,H, Wang,Q
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055
Resumo: ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle.
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network_name_str Brazilian Journal of Poultry Science (Online)
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spelling Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast MuscleFeeding conditionschickenFATP1meat qualityintramuscular fatABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle.Fundacao de Apoio a Ciência e Tecnologia Avicolas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055Brazilian Journal of Poultry Science v.19 n.spe 2017reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/1806-9061-2016-0367info:eu-repo/semantics/openAccessGao,GWang,HZhao,XLi,QWang,CLi,JZhang,KZhong,HWang,Qeng2017-05-18T00:00:00Zoai:scielo:S1516-635X2017000500055Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2017-05-18T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
title Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
spellingShingle Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
Gao,G
Feeding conditions
chicken
FATP1
meat quality
intramuscular fat
title_short Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
title_full Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
title_fullStr Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
title_full_unstemmed Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
title_sort Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
author Gao,G
author_facet Gao,G
Wang,H
Zhao,X
Li,Q
Wang,C
Li,J
Zhang,K
Zhong,H
Wang,Q
author_role author
author2 Wang,H
Zhao,X
Li,Q
Wang,C
Li,J
Zhang,K
Zhong,H
Wang,Q
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gao,G
Wang,H
Zhao,X
Li,Q
Wang,C
Li,J
Zhang,K
Zhong,H
Wang,Q
dc.subject.por.fl_str_mv Feeding conditions
chicken
FATP1
meat quality
intramuscular fat
topic Feeding conditions
chicken
FATP1
meat quality
intramuscular fat
description ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9061-2016-0367
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.19 n.spe 2017
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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