Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biota Neotropica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000300105 |
Resumo: | This paper presents results from a study of albumin from pacu (Piaractus mesopotamicus, Holmberg 1887) and the catfish pintado (Pseudoplatystoma corruscans, Spix & Agassiz, 1829), two neotropical fish species inhabitants of Brazilian rivers, comparing their molecular mass and discussing their secondary structures based on spectropolarimetric (circular dychroism) measurements. Genetic controlled specimens were obtained from two fish hatcheries, located in Mococa (pacu) and in São João da Boa Vista (pintado), both in São Paulo State, Brazil. After a period of adaptation in holding tanks, fish blood samples were taken by punctioning their abdominal aorta. Purified albumin was obtained by gel filtration. SDS-PAGE electrophoresis was performed for the molecular mass estimation. Circular Dichroism spectra were registered for albumins of the two fish species over the range of 190-250 nm (far-UV), which shown two negative bands at 217 and 208 nm, a positive peak at 196 nm and a crossover at 200 nm. This profile is compatible with proteins that content predominantly alpha-helix structure. |
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Biota Neotropica |
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Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, BrazilSpotted sorubimPseudoplatystoma corruscansPacuPiaractus mesopotamicusfish serum albumincircular dychroismThis paper presents results from a study of albumin from pacu (Piaractus mesopotamicus, Holmberg 1887) and the catfish pintado (Pseudoplatystoma corruscans, Spix & Agassiz, 1829), two neotropical fish species inhabitants of Brazilian rivers, comparing their molecular mass and discussing their secondary structures based on spectropolarimetric (circular dychroism) measurements. Genetic controlled specimens were obtained from two fish hatcheries, located in Mococa (pacu) and in São João da Boa Vista (pintado), both in São Paulo State, Brazil. After a period of adaptation in holding tanks, fish blood samples were taken by punctioning their abdominal aorta. Purified albumin was obtained by gel filtration. SDS-PAGE electrophoresis was performed for the molecular mass estimation. Circular Dichroism spectra were registered for albumins of the two fish species over the range of 190-250 nm (far-UV), which shown two negative bands at 217 and 208 nm, a positive peak at 196 nm and a crossover at 200 nm. This profile is compatible with proteins that content predominantly alpha-helix structure.Instituto Virtual da Biodiversidade | BIOTA - FAPESP2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000300105Biota Neotropica v.15 n.3 2015reponame:Biota Neotropicainstname:Instituto Virtual da Biodiversidade (BIOTA-FAPESP)instacron:BIOTA - FAPESP10.1590/1676-0611-BN-2014-0179info:eu-repo/semantics/openAccessSilva,DilsonCortez,Celia MartinsNogueira,Natalia P.Bastos,Frederico Freire da Cunhaeng2015-11-19T00:00:00Zoai:scielo:S1676-06032015000300105Revistahttps://www.biotaneotropica.org.br/v20n1/pt/https://old.scielo.br/oai/scielo-oai.php||juliosa@unifap.br1676-06111676-0611opendoar:2015-11-19T00:00Biota Neotropica - Instituto Virtual da Biodiversidade (BIOTA-FAPESP)false |
dc.title.none.fl_str_mv |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
title |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
spellingShingle |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil Silva,Dilson Spotted sorubim Pseudoplatystoma corruscans Pacu Piaractus mesopotamicus fish serum albumin circular dychroism |
title_short |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
title_full |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
title_fullStr |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
title_full_unstemmed |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
title_sort |
Purification and physico-chemical study of serum albumins of two neotropical fish species from the São Francisco River Bassin, Brazil |
author |
Silva,Dilson |
author_facet |
Silva,Dilson Cortez,Celia Martins Nogueira,Natalia P. Bastos,Frederico Freire da Cunha |
author_role |
author |
author2 |
Cortez,Celia Martins Nogueira,Natalia P. Bastos,Frederico Freire da Cunha |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva,Dilson Cortez,Celia Martins Nogueira,Natalia P. Bastos,Frederico Freire da Cunha |
dc.subject.por.fl_str_mv |
Spotted sorubim Pseudoplatystoma corruscans Pacu Piaractus mesopotamicus fish serum albumin circular dychroism |
topic |
Spotted sorubim Pseudoplatystoma corruscans Pacu Piaractus mesopotamicus fish serum albumin circular dychroism |
description |
This paper presents results from a study of albumin from pacu (Piaractus mesopotamicus, Holmberg 1887) and the catfish pintado (Pseudoplatystoma corruscans, Spix & Agassiz, 1829), two neotropical fish species inhabitants of Brazilian rivers, comparing their molecular mass and discussing their secondary structures based on spectropolarimetric (circular dychroism) measurements. Genetic controlled specimens were obtained from two fish hatcheries, located in Mococa (pacu) and in São João da Boa Vista (pintado), both in São Paulo State, Brazil. After a period of adaptation in holding tanks, fish blood samples were taken by punctioning their abdominal aorta. Purified albumin was obtained by gel filtration. SDS-PAGE electrophoresis was performed for the molecular mass estimation. Circular Dichroism spectra were registered for albumins of the two fish species over the range of 190-250 nm (far-UV), which shown two negative bands at 217 and 208 nm, a positive peak at 196 nm and a crossover at 200 nm. This profile is compatible with proteins that content predominantly alpha-helix structure. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000300105 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000300105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1676-0611-BN-2014-0179 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
publisher.none.fl_str_mv |
Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
dc.source.none.fl_str_mv |
Biota Neotropica v.15 n.3 2015 reponame:Biota Neotropica instname:Instituto Virtual da Biodiversidade (BIOTA-FAPESP) instacron:BIOTA - FAPESP |
instname_str |
Instituto Virtual da Biodiversidade (BIOTA-FAPESP) |
instacron_str |
BIOTA - FAPESP |
institution |
BIOTA - FAPESP |
reponame_str |
Biota Neotropica |
collection |
Biota Neotropica |
repository.name.fl_str_mv |
Biota Neotropica - Instituto Virtual da Biodiversidade (BIOTA-FAPESP) |
repository.mail.fl_str_mv |
||juliosa@unifap.br |
_version_ |
1754575900096069632 |