Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biota Neotropica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000200108 |
Resumo: | The occurrence of associations between bacteria and plant roots may be beneficial, neutral or detrimental. Plant growth promoting (PGP) bacteria form a heterogeneous group of beneficial microorganisms that can be found in the rhizosphere, the root surfaces or in association with host plant. The aim of this study was to isolate and characterize PGP bacteria associated to barley plants (Hordeum vulgare L.) aiming a future application as agricultural inoculant. One hundred and sixty bacterial strains were isolated from roots or rhizospheric soil of barley based on their growth in nitrogen-free selective media. They were evaluated for their ability to produce indolic compounds (ICs) and siderophores, and to solubilize tricalcium phosphate inin vitro assays. Most of them (74%) were able to synthesize ICs in the presence of the precursor L-tryptophan, while 57% of the isolates produced siderophores in Fe-limited liquid medium, and 17% were able to solubilize tricalcium phosphate. Thirty-two isolates possessing different PGP characteristics were identified by partial sequencing of their 16S rRNA gene. Strains belonging to Cedecea andMicrobacterium genera promoted the growth of barley plants in insoluble phosphate conditions, indicating that these bacteria could be used as bioinoculants contributing to decrease the amount of fertilizers applied in barley crops. |
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Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazilbacterial 16S rRNA geneinoculantbarleyphosphate solubilizationThe occurrence of associations between bacteria and plant roots may be beneficial, neutral or detrimental. Plant growth promoting (PGP) bacteria form a heterogeneous group of beneficial microorganisms that can be found in the rhizosphere, the root surfaces or in association with host plant. The aim of this study was to isolate and characterize PGP bacteria associated to barley plants (Hordeum vulgare L.) aiming a future application as agricultural inoculant. One hundred and sixty bacterial strains were isolated from roots or rhizospheric soil of barley based on their growth in nitrogen-free selective media. They were evaluated for their ability to produce indolic compounds (ICs) and siderophores, and to solubilize tricalcium phosphate inin vitro assays. Most of them (74%) were able to synthesize ICs in the presence of the precursor L-tryptophan, while 57% of the isolates produced siderophores in Fe-limited liquid medium, and 17% were able to solubilize tricalcium phosphate. Thirty-two isolates possessing different PGP characteristics were identified by partial sequencing of their 16S rRNA gene. Strains belonging to Cedecea andMicrobacterium genera promoted the growth of barley plants in insoluble phosphate conditions, indicating that these bacteria could be used as bioinoculants contributing to decrease the amount of fertilizers applied in barley crops.Instituto Virtual da Biodiversidade | BIOTA - FAPESP2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000200108Biota Neotropica v.15 n.2 2015reponame:Biota Neotropicainstname:Instituto Virtual da Biodiversidade (BIOTA-FAPESP)instacron:BIOTA - FAPESP10.1590/1676-06032015010514info:eu-repo/semantics/openAccessPontes,Andress P.Souza,Rocheli deGranada,Camille E.Passaglia,Luciane M.P.eng2015-11-26T00:00:00Zoai:scielo:S1676-06032015000200108Revistahttps://www.biotaneotropica.org.br/v20n1/pt/https://old.scielo.br/oai/scielo-oai.php||juliosa@unifap.br1676-06111676-0611opendoar:2015-11-26T00:00Biota Neotropica - Instituto Virtual da Biodiversidade (BIOTA-FAPESP)false |
dc.title.none.fl_str_mv |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
title |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
spellingShingle |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil Pontes,Andress P. bacterial 16S rRNA gene inoculant barley phosphate solubilization |
title_short |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
title_full |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
title_fullStr |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
title_full_unstemmed |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
title_sort |
Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil |
author |
Pontes,Andress P. |
author_facet |
Pontes,Andress P. Souza,Rocheli de Granada,Camille E. Passaglia,Luciane M.P. |
author_role |
author |
author2 |
Souza,Rocheli de Granada,Camille E. Passaglia,Luciane M.P. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pontes,Andress P. Souza,Rocheli de Granada,Camille E. Passaglia,Luciane M.P. |
dc.subject.por.fl_str_mv |
bacterial 16S rRNA gene inoculant barley phosphate solubilization |
topic |
bacterial 16S rRNA gene inoculant barley phosphate solubilization |
description |
The occurrence of associations between bacteria and plant roots may be beneficial, neutral or detrimental. Plant growth promoting (PGP) bacteria form a heterogeneous group of beneficial microorganisms that can be found in the rhizosphere, the root surfaces or in association with host plant. The aim of this study was to isolate and characterize PGP bacteria associated to barley plants (Hordeum vulgare L.) aiming a future application as agricultural inoculant. One hundred and sixty bacterial strains were isolated from roots or rhizospheric soil of barley based on their growth in nitrogen-free selective media. They were evaluated for their ability to produce indolic compounds (ICs) and siderophores, and to solubilize tricalcium phosphate inin vitro assays. Most of them (74%) were able to synthesize ICs in the presence of the precursor L-tryptophan, while 57% of the isolates produced siderophores in Fe-limited liquid medium, and 17% were able to solubilize tricalcium phosphate. Thirty-two isolates possessing different PGP characteristics were identified by partial sequencing of their 16S rRNA gene. Strains belonging to Cedecea andMicrobacterium genera promoted the growth of barley plants in insoluble phosphate conditions, indicating that these bacteria could be used as bioinoculants contributing to decrease the amount of fertilizers applied in barley crops. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000200108 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000200108 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1676-06032015010514 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
publisher.none.fl_str_mv |
Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
dc.source.none.fl_str_mv |
Biota Neotropica v.15 n.2 2015 reponame:Biota Neotropica instname:Instituto Virtual da Biodiversidade (BIOTA-FAPESP) instacron:BIOTA - FAPESP |
instname_str |
Instituto Virtual da Biodiversidade (BIOTA-FAPESP) |
instacron_str |
BIOTA - FAPESP |
institution |
BIOTA - FAPESP |
reponame_str |
Biota Neotropica |
collection |
Biota Neotropica |
repository.name.fl_str_mv |
Biota Neotropica - Instituto Virtual da Biodiversidade (BIOTA-FAPESP) |
repository.mail.fl_str_mv |
||juliosa@unifap.br |
_version_ |
1754575900065660928 |