Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats

Detalhes bibliográficos
Autor(a) principal: Oliveira,Jussara Celi Conceição
Data de Publicação: 2019
Outros Autores: Steiner,Marcelo Luis, Theodoro,Thérèse Rachell, Mader,Ana Maria Amaral Antonio, Petri,Giuliana, Abreu,Luiz Carlos, Pinhal,Maria Aparecida da Silva, Fernandes,César Eduardo, Pompei,Luciano Melo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de ginecologia e obstetrícia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703
Resumo: Abstract Objective To investigate the action of testosterone (T), isolated or associated with estradiol benzoate (EB), on the proliferation markers and apoptosis of breasts of ovariectomized rats. Methods A total of 48 castrated female Wistar rats were divided into 6 groups, and each of them were submitted to one of the following treatments for 5 weeks: 1) control; 2) EB 50 mcg/day + T 50 mcg/day; 3) T 50mcg/day; 4) EB 50 mcg +T 300 mcg/day; 5) T 300 mcg/day; and 6) EB 50 mcg/day. After the treatment, the mammary tissue was submitted to a histological analysis and immunoexpression evaluation of proliferation markers (proliferating cell nuclear antigen, PCNA) and apoptosis (caspase-3). Results There was a statistically significant difference among the groups regarding microcalcifications and secretory activity, with higher prevalence in the groups treated with EB. There was no difference among the groups regarding atrophy, but a higher prevalence of atrophy was found in the groups that received T versus those that received EB +T. There was a difference among the groups regarding the PCNA (p = 0.028), with higher expression in the group submitted to EB +T 300 mcg/day. Regarding caspase-3, there was no difference among the groups; however, in the group submitted to EB +T 300 mcg/day, the expression was higher than in the isolated T group. Conclusion Isolated T did not have a proliferative effect on the mammary tissue, contrary to EB. Testosterone in combination with EB may or may not decrease the proliferation, depending on the dose of T.
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spelling Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized RatstestosteroneproliferationapoptosisbreastovariectomyAbstract Objective To investigate the action of testosterone (T), isolated or associated with estradiol benzoate (EB), on the proliferation markers and apoptosis of breasts of ovariectomized rats. Methods A total of 48 castrated female Wistar rats were divided into 6 groups, and each of them were submitted to one of the following treatments for 5 weeks: 1) control; 2) EB 50 mcg/day + T 50 mcg/day; 3) T 50mcg/day; 4) EB 50 mcg +T 300 mcg/day; 5) T 300 mcg/day; and 6) EB 50 mcg/day. After the treatment, the mammary tissue was submitted to a histological analysis and immunoexpression evaluation of proliferation markers (proliferating cell nuclear antigen, PCNA) and apoptosis (caspase-3). Results There was a statistically significant difference among the groups regarding microcalcifications and secretory activity, with higher prevalence in the groups treated with EB. There was no difference among the groups regarding atrophy, but a higher prevalence of atrophy was found in the groups that received T versus those that received EB +T. There was a difference among the groups regarding the PCNA (p = 0.028), with higher expression in the group submitted to EB +T 300 mcg/day. Regarding caspase-3, there was no difference among the groups; however, in the group submitted to EB +T 300 mcg/day, the expression was higher than in the isolated T group. Conclusion Isolated T did not have a proliferative effect on the mammary tissue, contrary to EB. Testosterone in combination with EB may or may not decrease the proliferation, depending on the dose of T.Federação Brasileira das Sociedades de Ginecologia e Obstetrícia2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703Revista Brasileira de Ginecologia e Obstetrícia v.41 n.12 2019reponame:Revista brasileira de ginecologia e obstetrícia (Online)instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)instacron:FEBRASGO10.1055/s-0039-3399552info:eu-repo/semantics/openAccessOliveira,Jussara Celi ConceiçãoSteiner,Marcelo LuisTheodoro,Thérèse RachellMader,Ana Maria Amaral AntonioPetri,GiulianaAbreu,Luiz CarlosPinhal,Maria Aparecida da SilvaFernandes,César EduardoPompei,Luciano Meloeng2020-01-29T00:00:00Zoai:scielo:S0100-72032019001200703Revistahttp://www.scielo.br/rbgohttps://old.scielo.br/oai/scielo-oai.phppublicações@febrasgo.org.br||rbgo@fmrp.usp.br1806-93390100-7203opendoar:2020-01-29T00:00Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)false
dc.title.none.fl_str_mv Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
title Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
spellingShingle Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
Oliveira,Jussara Celi Conceição
testosterone
proliferation
apoptosis
breast
ovariectomy
title_short Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
title_full Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
title_fullStr Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
title_full_unstemmed Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
title_sort Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
author Oliveira,Jussara Celi Conceição
author_facet Oliveira,Jussara Celi Conceição
Steiner,Marcelo Luis
Theodoro,Thérèse Rachell
Mader,Ana Maria Amaral Antonio
Petri,Giuliana
Abreu,Luiz Carlos
Pinhal,Maria Aparecida da Silva
Fernandes,César Eduardo
Pompei,Luciano Melo
author_role author
author2 Steiner,Marcelo Luis
Theodoro,Thérèse Rachell
Mader,Ana Maria Amaral Antonio
Petri,Giuliana
Abreu,Luiz Carlos
Pinhal,Maria Aparecida da Silva
Fernandes,César Eduardo
Pompei,Luciano Melo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Jussara Celi Conceição
Steiner,Marcelo Luis
Theodoro,Thérèse Rachell
Mader,Ana Maria Amaral Antonio
Petri,Giuliana
Abreu,Luiz Carlos
Pinhal,Maria Aparecida da Silva
Fernandes,César Eduardo
Pompei,Luciano Melo
dc.subject.por.fl_str_mv testosterone
proliferation
apoptosis
breast
ovariectomy
topic testosterone
proliferation
apoptosis
breast
ovariectomy
description Abstract Objective To investigate the action of testosterone (T), isolated or associated with estradiol benzoate (EB), on the proliferation markers and apoptosis of breasts of ovariectomized rats. Methods A total of 48 castrated female Wistar rats were divided into 6 groups, and each of them were submitted to one of the following treatments for 5 weeks: 1) control; 2) EB 50 mcg/day + T 50 mcg/day; 3) T 50mcg/day; 4) EB 50 mcg +T 300 mcg/day; 5) T 300 mcg/day; and 6) EB 50 mcg/day. After the treatment, the mammary tissue was submitted to a histological analysis and immunoexpression evaluation of proliferation markers (proliferating cell nuclear antigen, PCNA) and apoptosis (caspase-3). Results There was a statistically significant difference among the groups regarding microcalcifications and secretory activity, with higher prevalence in the groups treated with EB. There was no difference among the groups regarding atrophy, but a higher prevalence of atrophy was found in the groups that received T versus those that received EB +T. There was a difference among the groups regarding the PCNA (p = 0.028), with higher expression in the group submitted to EB +T 300 mcg/day. Regarding caspase-3, there was no difference among the groups; however, in the group submitted to EB +T 300 mcg/day, the expression was higher than in the isolated T group. Conclusion Isolated T did not have a proliferative effect on the mammary tissue, contrary to EB. Testosterone in combination with EB may or may not decrease the proliferation, depending on the dose of T.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1055/s-0039-3399552
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
publisher.none.fl_str_mv Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
dc.source.none.fl_str_mv Revista Brasileira de Ginecologia e Obstetrícia v.41 n.12 2019
reponame:Revista brasileira de ginecologia e obstetrícia (Online)
instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)
instacron:FEBRASGO
instname_str Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)
instacron_str FEBRASGO
institution FEBRASGO
reponame_str Revista brasileira de ginecologia e obstetrícia (Online)
collection Revista brasileira de ginecologia e obstetrícia (Online)
repository.name.fl_str_mv Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)
repository.mail.fl_str_mv publicações@febrasgo.org.br||rbgo@fmrp.usp.br
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