Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de ginecologia e obstetrícia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703 |
Resumo: | Abstract Objective To investigate the action of testosterone (T), isolated or associated with estradiol benzoate (EB), on the proliferation markers and apoptosis of breasts of ovariectomized rats. Methods A total of 48 castrated female Wistar rats were divided into 6 groups, and each of them were submitted to one of the following treatments for 5 weeks: 1) control; 2) EB 50 mcg/day + T 50 mcg/day; 3) T 50mcg/day; 4) EB 50 mcg +T 300 mcg/day; 5) T 300 mcg/day; and 6) EB 50 mcg/day. After the treatment, the mammary tissue was submitted to a histological analysis and immunoexpression evaluation of proliferation markers (proliferating cell nuclear antigen, PCNA) and apoptosis (caspase-3). Results There was a statistically significant difference among the groups regarding microcalcifications and secretory activity, with higher prevalence in the groups treated with EB. There was no difference among the groups regarding atrophy, but a higher prevalence of atrophy was found in the groups that received T versus those that received EB +T. There was a difference among the groups regarding the PCNA (p = 0.028), with higher expression in the group submitted to EB +T 300 mcg/day. Regarding caspase-3, there was no difference among the groups; however, in the group submitted to EB +T 300 mcg/day, the expression was higher than in the isolated T group. Conclusion Isolated T did not have a proliferative effect on the mammary tissue, contrary to EB. Testosterone in combination with EB may or may not decrease the proliferation, depending on the dose of T. |
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Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized RatstestosteroneproliferationapoptosisbreastovariectomyAbstract Objective To investigate the action of testosterone (T), isolated or associated with estradiol benzoate (EB), on the proliferation markers and apoptosis of breasts of ovariectomized rats. Methods A total of 48 castrated female Wistar rats were divided into 6 groups, and each of them were submitted to one of the following treatments for 5 weeks: 1) control; 2) EB 50 mcg/day + T 50 mcg/day; 3) T 50mcg/day; 4) EB 50 mcg +T 300 mcg/day; 5) T 300 mcg/day; and 6) EB 50 mcg/day. After the treatment, the mammary tissue was submitted to a histological analysis and immunoexpression evaluation of proliferation markers (proliferating cell nuclear antigen, PCNA) and apoptosis (caspase-3). Results There was a statistically significant difference among the groups regarding microcalcifications and secretory activity, with higher prevalence in the groups treated with EB. There was no difference among the groups regarding atrophy, but a higher prevalence of atrophy was found in the groups that received T versus those that received EB +T. There was a difference among the groups regarding the PCNA (p = 0.028), with higher expression in the group submitted to EB +T 300 mcg/day. Regarding caspase-3, there was no difference among the groups; however, in the group submitted to EB +T 300 mcg/day, the expression was higher than in the isolated T group. Conclusion Isolated T did not have a proliferative effect on the mammary tissue, contrary to EB. Testosterone in combination with EB may or may not decrease the proliferation, depending on the dose of T.Federação Brasileira das Sociedades de Ginecologia e Obstetrícia2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703Revista Brasileira de Ginecologia e Obstetrícia v.41 n.12 2019reponame:Revista brasileira de ginecologia e obstetrícia (Online)instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)instacron:FEBRASGO10.1055/s-0039-3399552info:eu-repo/semantics/openAccessOliveira,Jussara Celi ConceiçãoSteiner,Marcelo LuisTheodoro,Thérèse RachellMader,Ana Maria Amaral AntonioPetri,GiulianaAbreu,Luiz CarlosPinhal,Maria Aparecida da SilvaFernandes,César EduardoPompei,Luciano Meloeng2020-01-29T00:00:00Zoai:scielo:S0100-72032019001200703Revistahttp://www.scielo.br/rbgohttps://old.scielo.br/oai/scielo-oai.phppublicações@febrasgo.org.br||rbgo@fmrp.usp.br1806-93390100-7203opendoar:2020-01-29T00:00Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)false |
dc.title.none.fl_str_mv |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
title |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
spellingShingle |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats Oliveira,Jussara Celi Conceição testosterone proliferation apoptosis breast ovariectomy |
title_short |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
title_full |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
title_fullStr |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
title_full_unstemmed |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
title_sort |
Effect of Testosterone on Proliferation Markers and Apoptosis in Breasts of Ovariectomized Rats |
author |
Oliveira,Jussara Celi Conceição |
author_facet |
Oliveira,Jussara Celi Conceição Steiner,Marcelo Luis Theodoro,Thérèse Rachell Mader,Ana Maria Amaral Antonio Petri,Giuliana Abreu,Luiz Carlos Pinhal,Maria Aparecida da Silva Fernandes,César Eduardo Pompei,Luciano Melo |
author_role |
author |
author2 |
Steiner,Marcelo Luis Theodoro,Thérèse Rachell Mader,Ana Maria Amaral Antonio Petri,Giuliana Abreu,Luiz Carlos Pinhal,Maria Aparecida da Silva Fernandes,César Eduardo Pompei,Luciano Melo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Jussara Celi Conceição Steiner,Marcelo Luis Theodoro,Thérèse Rachell Mader,Ana Maria Amaral Antonio Petri,Giuliana Abreu,Luiz Carlos Pinhal,Maria Aparecida da Silva Fernandes,César Eduardo Pompei,Luciano Melo |
dc.subject.por.fl_str_mv |
testosterone proliferation apoptosis breast ovariectomy |
topic |
testosterone proliferation apoptosis breast ovariectomy |
description |
Abstract Objective To investigate the action of testosterone (T), isolated or associated with estradiol benzoate (EB), on the proliferation markers and apoptosis of breasts of ovariectomized rats. Methods A total of 48 castrated female Wistar rats were divided into 6 groups, and each of them were submitted to one of the following treatments for 5 weeks: 1) control; 2) EB 50 mcg/day + T 50 mcg/day; 3) T 50mcg/day; 4) EB 50 mcg +T 300 mcg/day; 5) T 300 mcg/day; and 6) EB 50 mcg/day. After the treatment, the mammary tissue was submitted to a histological analysis and immunoexpression evaluation of proliferation markers (proliferating cell nuclear antigen, PCNA) and apoptosis (caspase-3). Results There was a statistically significant difference among the groups regarding microcalcifications and secretory activity, with higher prevalence in the groups treated with EB. There was no difference among the groups regarding atrophy, but a higher prevalence of atrophy was found in the groups that received T versus those that received EB +T. There was a difference among the groups regarding the PCNA (p = 0.028), with higher expression in the group submitted to EB +T 300 mcg/day. Regarding caspase-3, there was no difference among the groups; however, in the group submitted to EB +T 300 mcg/day, the expression was higher than in the isolated T group. Conclusion Isolated T did not have a proliferative effect on the mammary tissue, contrary to EB. Testosterone in combination with EB may or may not decrease the proliferation, depending on the dose of T. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019001200703 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1055/s-0039-3399552 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
publisher.none.fl_str_mv |
Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
dc.source.none.fl_str_mv |
Revista Brasileira de Ginecologia e Obstetrícia v.41 n.12 2019 reponame:Revista brasileira de ginecologia e obstetrícia (Online) instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) instacron:FEBRASGO |
instname_str |
Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) |
instacron_str |
FEBRASGO |
institution |
FEBRASGO |
reponame_str |
Revista brasileira de ginecologia e obstetrícia (Online) |
collection |
Revista brasileira de ginecologia e obstetrícia (Online) |
repository.name.fl_str_mv |
Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) |
repository.mail.fl_str_mv |
publicações@febrasgo.org.br||rbgo@fmrp.usp.br |
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1754115944955772928 |