Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)

Detalhes bibliográficos
Autor(a) principal: Tirone,Nelson Ranieri
Data de Publicação: 2021
Outros Autores: Campos,Carolina Guissoni, Ferreira,Kézia Jesus Aguiar, Stark,Léticia Montes, Vieira,Jéssica Ferreira, Murta,Eddie Fernando Cândido, Michelin,Márcia Antoniazi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de ginecologia e obstetrícia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000900682
Resumo: Abstract Objective The aim of the present study was to compare the local and systemic expression of the factors linked to the interferon alpha (IFN-α) activation pathway in different degrees of cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods A total of 128 patients with CIN I, CIN II, CIN III and cervical cancer was evaluated. The real-time polymerase chain reaction (RT-PCR) technique was used to evaluate the gene expression of IFNR1, IFNR2, IFN-α, oligoadenylate synthase (2’5′OAS), cytokine signal suppressor 1 (SOCS) 1, SOCS3, signal transducer and transcription activator 1 (STAT1), and IRF9 from 128 biopsies. A total of 46 out of 128 samples were evaluated by flow cytometry for IFNAR1, IFNAR2, STAT1, IRF7 and IFN-α in peripheral blood cells. Results Patients with CIN II and III (63 samples) had a low local expression of IFNR1, but not IFNR2. Patients with some degree of injury showed high expression of SOCS1 and SOCS3. Systemically, patients with CIN II and III (20 samples) had a significant increase in IFNR1, IFNR2, STAT1, IRF7, and IFN-α in helper, cytotoxic T lymphocytes, and in monocytes. Conclusion Patients with high-grade lesions have increased systemic expression of IFN-α and its activation pathways in helper and cytotoxic T lymphocytes, as well as in monocytes due to an exacerbation of the immune response in these patients. This phenomenon is not accompanied by resolution of the lesion due to a defect in the IFN-α activation pathway that revealed by low local IFNAR1 expression and high local expression of SOCS1 and SOCS3.
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spelling Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)interferon receptorscervical intraepithelial neoplasiainterferon alphaAbstract Objective The aim of the present study was to compare the local and systemic expression of the factors linked to the interferon alpha (IFN-α) activation pathway in different degrees of cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods A total of 128 patients with CIN I, CIN II, CIN III and cervical cancer was evaluated. The real-time polymerase chain reaction (RT-PCR) technique was used to evaluate the gene expression of IFNR1, IFNR2, IFN-α, oligoadenylate synthase (2’5′OAS), cytokine signal suppressor 1 (SOCS) 1, SOCS3, signal transducer and transcription activator 1 (STAT1), and IRF9 from 128 biopsies. A total of 46 out of 128 samples were evaluated by flow cytometry for IFNAR1, IFNAR2, STAT1, IRF7 and IFN-α in peripheral blood cells. Results Patients with CIN II and III (63 samples) had a low local expression of IFNR1, but not IFNR2. Patients with some degree of injury showed high expression of SOCS1 and SOCS3. Systemically, patients with CIN II and III (20 samples) had a significant increase in IFNR1, IFNR2, STAT1, IRF7, and IFN-α in helper, cytotoxic T lymphocytes, and in monocytes. Conclusion Patients with high-grade lesions have increased systemic expression of IFN-α and its activation pathways in helper and cytotoxic T lymphocytes, as well as in monocytes due to an exacerbation of the immune response in these patients. This phenomenon is not accompanied by resolution of the lesion due to a defect in the IFN-α activation pathway that revealed by low local IFNAR1 expression and high local expression of SOCS1 and SOCS3.Federação Brasileira das Sociedades de Ginecologia e Obstetrícia2021-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000900682Revista Brasileira de Ginecologia e Obstetrícia v.43 n.9 2021reponame:Revista brasileira de ginecologia e obstetrícia (Online)instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)instacron:FEBRASGO10.1055/s-0041-1735301info:eu-repo/semantics/openAccessTirone,Nelson RanieriCampos,Carolina GuissoniFerreira,Kézia Jesus AguiarStark,Léticia MontesVieira,Jéssica FerreiraMurta,Eddie Fernando CândidoMichelin,Márcia Antoniazieng2021-11-25T00:00:00Zoai:scielo:S0100-72032021000900682Revistahttp://www.scielo.br/rbgohttps://old.scielo.br/oai/scielo-oai.phppublicações@febrasgo.org.br||rbgo@fmrp.usp.br1806-93390100-7203opendoar:2021-11-25T00:00Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)false
dc.title.none.fl_str_mv Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
title Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
spellingShingle Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
Tirone,Nelson Ranieri
interferon receptors
cervical intraepithelial neoplasia
interferon alpha
title_short Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
title_full Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
title_fullStr Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
title_full_unstemmed Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
title_sort Pathways of IFN-alpha Activation in Patients with Cervical Intraepithelial Neoplasia (CIN)
author Tirone,Nelson Ranieri
author_facet Tirone,Nelson Ranieri
Campos,Carolina Guissoni
Ferreira,Kézia Jesus Aguiar
Stark,Léticia Montes
Vieira,Jéssica Ferreira
Murta,Eddie Fernando Cândido
Michelin,Márcia Antoniazi
author_role author
author2 Campos,Carolina Guissoni
Ferreira,Kézia Jesus Aguiar
Stark,Léticia Montes
Vieira,Jéssica Ferreira
Murta,Eddie Fernando Cândido
Michelin,Márcia Antoniazi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tirone,Nelson Ranieri
Campos,Carolina Guissoni
Ferreira,Kézia Jesus Aguiar
Stark,Léticia Montes
Vieira,Jéssica Ferreira
Murta,Eddie Fernando Cândido
Michelin,Márcia Antoniazi
dc.subject.por.fl_str_mv interferon receptors
cervical intraepithelial neoplasia
interferon alpha
topic interferon receptors
cervical intraepithelial neoplasia
interferon alpha
description Abstract Objective The aim of the present study was to compare the local and systemic expression of the factors linked to the interferon alpha (IFN-α) activation pathway in different degrees of cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods A total of 128 patients with CIN I, CIN II, CIN III and cervical cancer was evaluated. The real-time polymerase chain reaction (RT-PCR) technique was used to evaluate the gene expression of IFNR1, IFNR2, IFN-α, oligoadenylate synthase (2’5′OAS), cytokine signal suppressor 1 (SOCS) 1, SOCS3, signal transducer and transcription activator 1 (STAT1), and IRF9 from 128 biopsies. A total of 46 out of 128 samples were evaluated by flow cytometry for IFNAR1, IFNAR2, STAT1, IRF7 and IFN-α in peripheral blood cells. Results Patients with CIN II and III (63 samples) had a low local expression of IFNR1, but not IFNR2. Patients with some degree of injury showed high expression of SOCS1 and SOCS3. Systemically, patients with CIN II and III (20 samples) had a significant increase in IFNR1, IFNR2, STAT1, IRF7, and IFN-α in helper, cytotoxic T lymphocytes, and in monocytes. Conclusion Patients with high-grade lesions have increased systemic expression of IFN-α and its activation pathways in helper and cytotoxic T lymphocytes, as well as in monocytes due to an exacerbation of the immune response in these patients. This phenomenon is not accompanied by resolution of the lesion due to a defect in the IFN-α activation pathway that revealed by low local IFNAR1 expression and high local expression of SOCS1 and SOCS3.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000900682
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021000900682
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1055/s-0041-1735301
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
publisher.none.fl_str_mv Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
dc.source.none.fl_str_mv Revista Brasileira de Ginecologia e Obstetrícia v.43 n.9 2021
reponame:Revista brasileira de ginecologia e obstetrícia (Online)
instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)
instacron:FEBRASGO
instname_str Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)
instacron_str FEBRASGO
institution FEBRASGO
reponame_str Revista brasileira de ginecologia e obstetrícia (Online)
collection Revista brasileira de ginecologia e obstetrícia (Online)
repository.name.fl_str_mv Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)
repository.mail.fl_str_mv publicações@febrasgo.org.br||rbgo@fmrp.usp.br
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