The Importance of Hyphenated Techniques (LV/UV, LC/MS, LC/NMR) in the Search of Active Principles

• Autor(a) principal: Queiroz, E. F.
• Data de Publicação: 2006
• Outros Autores: Hostettmann, K.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/58
• topic: Natural Products; Hyphenated Techniques; LC-UV; LC-MS; LCRMN; Produtos Naturais; Técnicas Acopladas; CL-UV; CL-MS; CLNMR

Nature represents an extraordinary reservoir of novel molecules and there is currently a resurgence of interest in natural products as a possible source of new lead compounds for introduction into therapeutical screening programmes. To discover new bioactive natural products, the dereplication of crude extracts performed prior to isolation work is of crucial importance for avoiding the tedious isolation of known constituents. In this respect, chemical screening strategies based on hyphenated techniques such as liquid chromatography-ultraviolet photodiode array detection (LC-UVDAD), liquid chromatography-mass spectrometry (LC-MS), and liquid chromatography-nuclear magnetic resonance (LC-NMR) are more and more extensively used. In the laboratory of Hostettmann’s group, these analytical methods have been fully integrated into the isolation process and are used for the chemical screening of crude plant extracts, in complement with online or at-line bioassays, for rapid localization and identification of new bioactive compounds. In this paper, possibilities and limitations of hyphenated techniques for online natural product identification are discussed. As LC-NMR is playing a key role in this respect, the main part of the paper is dedicated to this technique. In particular, various ways of integrating NMR in the dereplication process are illustrated.