The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Fitos |
Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146 |
Resumo: | The Schinus terebinthifolius Raddi, known as aroeira da praia, is used in traditional medicine for the treatment of lesions and ulcers in the skin and mucosa, infections in the respiratory threat, digestive and urinary systems. One of the biggest problems faced by the fitotherapic industry is the microbian contamination of the drug. This work aimed at assessing the influence of the autoclavation and microwave processes on the microbian contamination, and the total content of tannins and flavanoids of the powder and hydroalcoholic extracts of the aroeira. The extracts were obtained by maceration in the proportion of 1:10 of plant/solvent with alcohol at 40%, 50%, 60% and 70%. The microbiologic methods used were the microorganism count on a dish by pour plate and the pathogeneous research, performing a triplicate analysis of the samples. On the microbian count after autoclavation, no fungal growth was observed, but there was bacterial growth (0.12 x 102 UFC/g). In the microwave process bacterial growth occurred (0.50 x 102 UFC/g) as well as fungal growth (0.10 x 102 UFC/g). The powder, when untreated, show greater bacterial growth (3.68 x 102 UFC/g) and fungi (0.26 x 102 UFC/g). The extracts treated by alcohol showed less microbian contamination. The sterilization process by autoclavation did not lead to a decrease in the polyphenols and total tannins contents. |
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The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius RaddiInfluência dos Processos de Autoclavação e Microondas Sobre a Carga Microbiana e Teor de Polifenóis e Taninos Totais das Cascas de Schinus terebinthifolius RaddiSchinus terebinthifolius Raddiautoclavaçãomicroondastaninos e polifenóis totaisCarga microbianaSchinus terebinthifolius Raddiautoclavationmicrowavetotal content of polyphenols and tanninsThe Schinus terebinthifolius Raddi, known as aroeira da praia, is used in traditional medicine for the treatment of lesions and ulcers in the skin and mucosa, infections in the respiratory threat, digestive and urinary systems. One of the biggest problems faced by the fitotherapic industry is the microbian contamination of the drug. This work aimed at assessing the influence of the autoclavation and microwave processes on the microbian contamination, and the total content of tannins and flavanoids of the powder and hydroalcoholic extracts of the aroeira. The extracts were obtained by maceration in the proportion of 1:10 of plant/solvent with alcohol at 40%, 50%, 60% and 70%. The microbiologic methods used were the microorganism count on a dish by pour plate and the pathogeneous research, performing a triplicate analysis of the samples. On the microbian count after autoclavation, no fungal growth was observed, but there was bacterial growth (0.12 x 102 UFC/g). In the microwave process bacterial growth occurred (0.50 x 102 UFC/g) as well as fungal growth (0.10 x 102 UFC/g). The powder, when untreated, show greater bacterial growth (3.68 x 102 UFC/g) and fungi (0.26 x 102 UFC/g). The extracts treated by alcohol showed less microbian contamination. The sterilization process by autoclavation did not lead to a decrease in the polyphenols and total tannins contents.A espécie Schinus terebinthifolius Raddi, conhecida como aroeira da praia, é utilizada na medicina tradicional para o tratamento de lesões e úlceras de pele e mucosas, infecções do sistema respiratório, digestivo e geniturinário. Sendo um dos maiores problemas enfrentados pela indústria de fitoterápicos a contaminação microbiana das matérias-primas, este trabalho objetivou avaliar a influência dos processos de autoclavação e microondas sobre a contaminação microbiológica e teor de taninos e flavonóides totais do pó e de extratos hidroalcoólicos de aroeira. Os extratos foram obtidos por maceração na proporção de 1:10 de planta/solvente com álcool a 40%, 50%, 60% e 70%. Os métodos microbiológicos utilizados foram o de contagem de microrganismos em placa por pour plate e o da pesquisa de patógenos, analisando em triplicata cada uma das amostras. Nos pós autoclavados houve apenas crescimento bacteriano (0,12 x 102 UFC/g). No processo por microondas ocorreu crescimento para bactérias (0,50 x 102 UFC/g) e fungos (0,10 x 102 UFC/g). Os pós quando não tratados, apresentaram maior crescimento de bactérias (3,68 x 102 UFC/g) e fungos (0,26 x 102 UFC/g). Os extratos tratados com álcool apresentaram menor contaminação microbiana. O processo de esterilização por autoclave não acarretou redução de polifenóis e taninos totais.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2012-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/14610.32712/2446-4775.2012.146Revista Fitos; Vol. 7 No. 02 (2012); 125-130Revista Fitos; Vol. 7 Núm. 02 (2012); 125-130Revista Fitos; v. 7 n. 02 (2012); 125-1302446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146/144https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146/1176Copyright (c) 2016 Revista Fitos Eletrônicainfo:eu-repo/semantics/openAccessMoura, Túlio Flávio A. L.Raffin, Fernanda N.Santos, Ana Lourdes R.Lavor, Edilene P.2022-10-06T13:09:47Zoai:ojs.revistafitos.far.fiocruz.br:article/146Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2022-10-06T13:09:47Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi Influência dos Processos de Autoclavação e Microondas Sobre a Carga Microbiana e Teor de Polifenóis e Taninos Totais das Cascas de Schinus terebinthifolius Raddi |
title |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi |
spellingShingle |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi Moura, Túlio Flávio A. L. Schinus terebinthifolius Raddi autoclavação microondas taninos e polifenóis totais Carga microbiana Schinus terebinthifolius Raddi autoclavation microwave total content of polyphenols and tannins |
title_short |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi |
title_full |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi |
title_fullStr |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi |
title_full_unstemmed |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi |
title_sort |
The Influence of the Autoclavation and Microwave Processes on the Microbiological Charge and the Polyphenols and Total Tannins Contents from the Barks of Schinus terebinthifolius Raddi |
author |
Moura, Túlio Flávio A. L. |
author_facet |
Moura, Túlio Flávio A. L. Raffin, Fernanda N. Santos, Ana Lourdes R. Lavor, Edilene P. |
author_role |
author |
author2 |
Raffin, Fernanda N. Santos, Ana Lourdes R. Lavor, Edilene P. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Moura, Túlio Flávio A. L. Raffin, Fernanda N. Santos, Ana Lourdes R. Lavor, Edilene P. |
dc.subject.por.fl_str_mv |
Schinus terebinthifolius Raddi autoclavação microondas taninos e polifenóis totais Carga microbiana Schinus terebinthifolius Raddi autoclavation microwave total content of polyphenols and tannins |
topic |
Schinus terebinthifolius Raddi autoclavação microondas taninos e polifenóis totais Carga microbiana Schinus terebinthifolius Raddi autoclavation microwave total content of polyphenols and tannins |
description |
The Schinus terebinthifolius Raddi, known as aroeira da praia, is used in traditional medicine for the treatment of lesions and ulcers in the skin and mucosa, infections in the respiratory threat, digestive and urinary systems. One of the biggest problems faced by the fitotherapic industry is the microbian contamination of the drug. This work aimed at assessing the influence of the autoclavation and microwave processes on the microbian contamination, and the total content of tannins and flavanoids of the powder and hydroalcoholic extracts of the aroeira. The extracts were obtained by maceration in the proportion of 1:10 of plant/solvent with alcohol at 40%, 50%, 60% and 70%. The microbiologic methods used were the microorganism count on a dish by pour plate and the pathogeneous research, performing a triplicate analysis of the samples. On the microbian count after autoclavation, no fungal growth was observed, but there was bacterial growth (0.12 x 102 UFC/g). In the microwave process bacterial growth occurred (0.50 x 102 UFC/g) as well as fungal growth (0.10 x 102 UFC/g). The powder, when untreated, show greater bacterial growth (3.68 x 102 UFC/g) and fungi (0.26 x 102 UFC/g). The extracts treated by alcohol showed less microbian contamination. The sterilization process by autoclavation did not lead to a decrease in the polyphenols and total tannins contents. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146 10.32712/2446-4775.2012.146 |
url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146 |
identifier_str_mv |
10.32712/2446-4775.2012.146 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146/144 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/146/1176 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Revista Fitos Eletrônica info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Revista Fitos Eletrônica |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
dc.source.none.fl_str_mv |
Revista Fitos; Vol. 7 No. 02 (2012); 125-130 Revista Fitos; Vol. 7 Núm. 02 (2012); 125-130 Revista Fitos; v. 7 n. 02 (2012); 125-130 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Revista Fitos |
collection |
Revista Fitos |
repository.name.fl_str_mv |
Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
_version_ |
1798313473758724096 |