In vitro germination and direct organogenesis from hypocotyledonary explants with inverted polarity of Psychotria ipecacuanha (Brot.) Stokes

• Autor(a) principal: da Silva, Maurecilne L.
• Data de Publicação: 2014
• Outros Autores: Azevedo, Aristéa A., da Silva, Gizelly M., de Carvalho, Ilio F., Rossi, Ana Aparecida B., Otoni, Wagner C.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/189
• topic: Poaia; Alcalóides; Planta medicinal; Cultivo in vitro; Histologia; Brotações adventícias; Poaia; Alkaloids; Medicinal plant; In vitro culture; Histology; Adventitious shoots

This study aimed to seed germination and adventitious shoot regeneration from Psychotria ipecacuanha inverted hypocotyls. The results showed a percentage of 84.17% of seeds germinated with normal seedlings development. The germination started after the second day of cultivation and was observed up to 70 days. After 40 days seedlings were obtained with average 10 cm in length, responding satisfactorily to the process of acclimatization under laboratory conditions. In hypocotyls with inverted polarity adventitious shoots were formed directly from the explant tissue in the region of contact with the medium, and on the hypocotyl portion out of contact with the medium. The use of hypocotyls with inverted polarity has not been previously reported for the species. The organogenesis occurred directly in the explants and the BA at 1 mg L-1 enhanced the induction of greater number of adventitious shoots. Anatomical analysis confirmed the formation of adventitious shoots by direct pathway. Elongated adventitious shoots were rooted in the presence of IBA. The highest percentage of rooting shoots occurred in the presence of 1.5 mg L-1 of this growth regulator.