Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Fitos |
Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545 |
Resumo: | Berberis empetrifolia is an endemic species from Chile that produces some bioactive compounds, highlighting the alkaloid berberine, which demonstrated antitumor properties. Taking into account the constant plant supply demand required for medicinal use, the establishment of in vitro propagation protocols allows plant multiplication, without affecting natural populations. Moreover, the in vitro germination is an efficient strategy to obtain axenic plants. The aim of this study was to establish the in vitro germination and to characterize seeds morphology and seedling development of B. empetrifolia. After decontamination of seeds, pre-germinative treatments were applied. Seed viability ranged from 75-90% and the embedding curve revealed permeable integument. The germination was characterized as epigeal phanerocotylar type. Seeds not submitted to pre-germinative treatments reached 10-30% germination rate (GR) and showed asynchronous germination. The most efficient pre-germinative treatment was the storage of seeds at 3˚C for 12 days followed by overnight soaking in GA3 solution associated to seed coat removal. This treatment allowed the seed dormancy to be overcome, reaching GR above 95% and resulting in synchronous germination. The in vitro germination protocol established for B.empetrifolia was efficient to produce phenotypically normal axenic seedlings, allowing their use as source of plant material for biotechnological, phytochemical and pharmacological studies. |
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Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae)DormancyGibberellic acidMedicinal plantPost-seminal developmentPre-germinative treatmentsBerberis empetrifolia is an endemic species from Chile that produces some bioactive compounds, highlighting the alkaloid berberine, which demonstrated antitumor properties. Taking into account the constant plant supply demand required for medicinal use, the establishment of in vitro propagation protocols allows plant multiplication, without affecting natural populations. Moreover, the in vitro germination is an efficient strategy to obtain axenic plants. The aim of this study was to establish the in vitro germination and to characterize seeds morphology and seedling development of B. empetrifolia. After decontamination of seeds, pre-germinative treatments were applied. Seed viability ranged from 75-90% and the embedding curve revealed permeable integument. The germination was characterized as epigeal phanerocotylar type. Seeds not submitted to pre-germinative treatments reached 10-30% germination rate (GR) and showed asynchronous germination. The most efficient pre-germinative treatment was the storage of seeds at 3˚C for 12 days followed by overnight soaking in GA3 solution associated to seed coat removal. This treatment allowed the seed dormancy to be overcome, reaching GR above 95% and resulting in synchronous germination. The in vitro germination protocol established for B.empetrifolia was efficient to produce phenotypically normal axenic seedlings, allowing their use as source of plant material for biotechnological, phytochemical and pharmacological studies.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2023-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/154510.32712/2446-4775.2023.1545Revista Fitos; Vol. 17 No. 4 (2023); 490-501Revista Fitos; Vol. 17 Núm. 4 (2023); 490-501Revista Fitos; v. 17 n. 4 (2023); 490-5012446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545/1430https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545/1431Copyright (c) 2023 Revista Fitoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Castro, Tatiana Carvalho de Santos, Danúsia da Silva Simões-Gurgel, ClaudiaMontiel-Eulefi, EnriqueHonorato, Marco ParedesAlbarello, Norma2024-01-24T15:18:42Zoai:ojs.revistafitos.far.fiocruz.br:article/1545Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2024-01-24T15:18:42Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
title |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
spellingShingle |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) Castro, Tatiana Carvalho de Dormancy Gibberellic acid Medicinal plant Post-seminal development Pre-germinative treatments |
title_short |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
title_full |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
title_fullStr |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
title_full_unstemmed |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
title_sort |
Morpho-physiological studies on in vitro germination of Berberis empetrifolia Lam. (Berberidaceae) |
author |
Castro, Tatiana Carvalho de |
author_facet |
Castro, Tatiana Carvalho de Santos, Danúsia da Silva Simões-Gurgel, Claudia Montiel-Eulefi, Enrique Honorato, Marco Paredes Albarello, Norma |
author_role |
author |
author2 |
Santos, Danúsia da Silva Simões-Gurgel, Claudia Montiel-Eulefi, Enrique Honorato, Marco Paredes Albarello, Norma |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Castro, Tatiana Carvalho de Santos, Danúsia da Silva Simões-Gurgel, Claudia Montiel-Eulefi, Enrique Honorato, Marco Paredes Albarello, Norma |
dc.subject.por.fl_str_mv |
Dormancy Gibberellic acid Medicinal plant Post-seminal development Pre-germinative treatments |
topic |
Dormancy Gibberellic acid Medicinal plant Post-seminal development Pre-germinative treatments |
description |
Berberis empetrifolia is an endemic species from Chile that produces some bioactive compounds, highlighting the alkaloid berberine, which demonstrated antitumor properties. Taking into account the constant plant supply demand required for medicinal use, the establishment of in vitro propagation protocols allows plant multiplication, without affecting natural populations. Moreover, the in vitro germination is an efficient strategy to obtain axenic plants. The aim of this study was to establish the in vitro germination and to characterize seeds morphology and seedling development of B. empetrifolia. After decontamination of seeds, pre-germinative treatments were applied. Seed viability ranged from 75-90% and the embedding curve revealed permeable integument. The germination was characterized as epigeal phanerocotylar type. Seeds not submitted to pre-germinative treatments reached 10-30% germination rate (GR) and showed asynchronous germination. The most efficient pre-germinative treatment was the storage of seeds at 3˚C for 12 days followed by overnight soaking in GA3 solution associated to seed coat removal. This treatment allowed the seed dormancy to be overcome, reaching GR above 95% and resulting in synchronous germination. The in vitro germination protocol established for B.empetrifolia was efficient to produce phenotypically normal axenic seedlings, allowing their use as source of plant material for biotechnological, phytochemical and pharmacological studies. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545 10.32712/2446-4775.2023.1545 |
url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545 |
identifier_str_mv |
10.32712/2446-4775.2023.1545 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545/1430 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1545/1431 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Revista Fitos https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Revista Fitos https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
dc.source.none.fl_str_mv |
Revista Fitos; Vol. 17 No. 4 (2023); 490-501 Revista Fitos; Vol. 17 Núm. 4 (2023); 490-501 Revista Fitos; v. 17 n. 4 (2023); 490-501 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Revista Fitos |
collection |
Revista Fitos |
repository.name.fl_str_mv |
Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
_version_ |
1798313476185128960 |