Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul

Detalhes bibliográficos
Autor(a) principal: de Moraes, Mariana Cristina
Data de Publicação: 2020
Outros Autores: Garcia, Nathália Olívia Sousa, Lessa, Bruno, Da Nóbrega, Andréa Bezerra, da Conceição, Edemilson Cardoso
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Fitos
Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929
Resumo: Given the applicability of Brosimum gaudichaudii extracts in the treatment of vitiligo, the aim of the study was to develop a high performance thin layer chromatographic (HPTLC) analytical method for the simultaneous quantification of psoralen and bergapten, present in the lyophilized extract of B. gaudichaudii, in order to become an alternative method for quality control of extracts produced from this species. The lyophilized extract was produced from the soft extract of B. gaudichaudii roots, prepared with 55 % (v/v) hydroalcoholic solution. The methodology for the analysis of bergapten and psoralen compounds present in the lyophilized extract was developed and validated by thin layer chromatography (TLC)/densitometry. The best resolution, separation and identification of the markers occurred in RP 18 F254 (Merck) silica chromatoplate and on the mobile phase composed of acetonitrile: water (60:40). The retention factors (RF) for psoralen and bergapten were 0.41 and 0.35, respectively. The developed method of analysis was linear, precise and accurate. Thus, TLC/densitometry or HPTLC proved to be an alternative technique for the identification and separation of thesefuranocoumarins, being important for the development of new products containing B. gaudichaudii extracts for the treatment of vitiligo.
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spelling Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii TréculDesenvolvimento e validação de um método por CCD/densitometria para quantificação de psoraleno e bergapteno em Brosimum gaudichaudii TréculMama-cadelaVitiligoPsoralenoBergaptenoCCDAEBrosimum gaudichaudiiVitiligoPsoralenBergaptenHPTLCGiven the applicability of Brosimum gaudichaudii extracts in the treatment of vitiligo, the aim of the study was to develop a high performance thin layer chromatographic (HPTLC) analytical method for the simultaneous quantification of psoralen and bergapten, present in the lyophilized extract of B. gaudichaudii, in order to become an alternative method for quality control of extracts produced from this species. The lyophilized extract was produced from the soft extract of B. gaudichaudii roots, prepared with 55 % (v/v) hydroalcoholic solution. The methodology for the analysis of bergapten and psoralen compounds present in the lyophilized extract was developed and validated by thin layer chromatography (TLC)/densitometry. The best resolution, separation and identification of the markers occurred in RP 18 F254 (Merck) silica chromatoplate and on the mobile phase composed of acetonitrile: water (60:40). The retention factors (RF) for psoralen and bergapten were 0.41 and 0.35, respectively. The developed method of analysis was linear, precise and accurate. Thus, TLC/densitometry or HPTLC proved to be an alternative technique for the identification and separation of thesefuranocoumarins, being important for the development of new products containing B. gaudichaudii extracts for the treatment of vitiligo.Diante da aplicabilidade dos extratos de Brosimum gaudichaudii no tratamento do vitiligo, o objetivo do estudo foi desenvolver um método analítico por cromatografia em camada delgada de alta eficiência (CCDAE) para a quantificação simultânea de psoraleno e bergapteno, presentes no extrato liofilizado de B. gaudichaudii, a fim de se tornar um método alternativo para o controle de qualidade dos extratos produzidos a partir desta espécie. O extrato liofilizado foi produzido a partir do extrato mole das raízes de B. gaudichaudii preparadas com solução hidroalcoólica a 55 % (v/v). A metodologia para análise dos compostos bergapteno e psoraleno, presentes no extrato liofilizado, foi desenvolvida e validada por cromatografia em camada delgada (CCD)/densitometria. A melhor resolução, separação e identificação dos marcadores, ocorreram em cromatoplacas de sílica RP 18 F254 (Merck) e fase móvel composta por acetonitrila: água 60:40. Os fatores de retenção (FR) para o psoraleno e o bergapteno foram 0,41 e 0,35, respectivamente. O método de análise desenvolvido se mostrou linear, preciso e exato. Desta forma, a densitometria por CCD ou CCDAE se mostrou uma técnica alternativa para identificação e separação destas furanocumarinas, sendo importante para o desenvolvimento de novos produtos contendo extratos de B. gaudicaudii para o tratamento do vitiligo.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2020-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/92910.32712/2446-4775.2020.929Revista Fitos; Vol. 14 No. 2 (2020); 165-176Revista Fitos; Vol. 14 Núm. 2 (2020); 165-176Revista Fitos; v. 14 n. 2 (2020); 165-1762446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929/710https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929/711Copyright (c) 2020 Revista Fitoshttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessde Moraes, Mariana CristinaGarcia, Nathália Olívia SousaLessa, BrunoDa Nóbrega, Andréa Bezerrada Conceição, Edemilson Cardoso2020-09-30T14:59:12Zoai:ojs.revistafitos.far.fiocruz.br:article/929Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2020-09-30T14:59:12Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
Desenvolvimento e validação de um método por CCD/densitometria para quantificação de psoraleno e bergapteno em Brosimum gaudichaudii Trécul
title Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
spellingShingle Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
de Moraes, Mariana Cristina
Mama-cadela
Vitiligo
Psoraleno
Bergapteno
CCDAE
Brosimum gaudichaudii
Vitiligo
Psoralen
Bergapten
HPTLC
title_short Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
title_full Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
title_fullStr Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
title_full_unstemmed Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
title_sort Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul
author de Moraes, Mariana Cristina
author_facet de Moraes, Mariana Cristina
Garcia, Nathália Olívia Sousa
Lessa, Bruno
Da Nóbrega, Andréa Bezerra
da Conceição, Edemilson Cardoso
author_role author
author2 Garcia, Nathália Olívia Sousa
Lessa, Bruno
Da Nóbrega, Andréa Bezerra
da Conceição, Edemilson Cardoso
author2_role author
author
author
author
dc.contributor.author.fl_str_mv de Moraes, Mariana Cristina
Garcia, Nathália Olívia Sousa
Lessa, Bruno
Da Nóbrega, Andréa Bezerra
da Conceição, Edemilson Cardoso
dc.subject.por.fl_str_mv Mama-cadela
Vitiligo
Psoraleno
Bergapteno
CCDAE
Brosimum gaudichaudii
Vitiligo
Psoralen
Bergapten
HPTLC
topic Mama-cadela
Vitiligo
Psoraleno
Bergapteno
CCDAE
Brosimum gaudichaudii
Vitiligo
Psoralen
Bergapten
HPTLC
description Given the applicability of Brosimum gaudichaudii extracts in the treatment of vitiligo, the aim of the study was to develop a high performance thin layer chromatographic (HPTLC) analytical method for the simultaneous quantification of psoralen and bergapten, present in the lyophilized extract of B. gaudichaudii, in order to become an alternative method for quality control of extracts produced from this species. The lyophilized extract was produced from the soft extract of B. gaudichaudii roots, prepared with 55 % (v/v) hydroalcoholic solution. The methodology for the analysis of bergapten and psoralen compounds present in the lyophilized extract was developed and validated by thin layer chromatography (TLC)/densitometry. The best resolution, separation and identification of the markers occurred in RP 18 F254 (Merck) silica chromatoplate and on the mobile phase composed of acetonitrile: water (60:40). The retention factors (RF) for psoralen and bergapten were 0.41 and 0.35, respectively. The developed method of analysis was linear, precise and accurate. Thus, TLC/densitometry or HPTLC proved to be an alternative technique for the identification and separation of thesefuranocoumarins, being important for the development of new products containing B. gaudichaudii extracts for the treatment of vitiligo.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929
10.32712/2446-4775.2020.929
url https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929
identifier_str_mv 10.32712/2446-4775.2020.929
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929/710
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929/711
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista Fitos
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista Fitos
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
dc.source.none.fl_str_mv Revista Fitos; Vol. 14 No. 2 (2020); 165-176
Revista Fitos; Vol. 14 Núm. 2 (2020); 165-176
Revista Fitos; v. 14 n. 2 (2020); 165-176
2446-4775
1808-9569
reponame:Revista Fitos
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Revista Fitos
collection Revista Fitos
repository.name.fl_str_mv Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br
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