In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Revista Fitos |
| Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950 |
Resumo: | The objective of this study was to carry out phytochemical analysis of the bark extract of Casearia javitensis and to evaluate its antipromastigote activity against Leishmania amazonensis. The ethanol extract (EE) was fractionated in open-column chromatography to obtain the hexane (FrHex), dichloromethane (FrDcm), ethyl acetate (FrAcOET) and methanol (FrMeOH) fractions. The EE and its fractions were analyzed by thin layer chromatography (TLC). FrDcm was analyzed in high performance liquid chromatography with diode array detection (HPLC-DAD). The antipromastigote assay of Leishmania amazonensis and the cytotoxicity test against the acute monocytic leukemia cell line (THP-1) were performed by the MTT colorimetric assay. The phytochemical profile in TLC detected terpenes in all samples. However, in the ultraviolet spectrum (HPLC-DAD) for FrDcm suggested the presence of a phenolic compound. FrHex and FrDcm showed antipromastigote activity (IC50 = 116.6 ± 0.9 and 59.4 ± 1.1 μg mL-1, respectively) and low cytotoxicity (CC50 = 333.4 ± 3.2 and 241.2 ± 1.9 μg mL-1, respectively). The selectivity index for FrDcm was 4.1. We conclude that the FrDcm of C. javitensis has potantial as a leishmanicide, and this activity may be related to the presence of phenolic compound. |
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In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae)Atividade antileishmanial in vitro e análise fitoquímica de Casearia javitensis Kunth (Salicaceae)LeishmanioseAntripromastigotaCitotoxicidade (THP-1)Composto FenólicoLeismaniasisAntipromastigoteCytotoxicity (THP-1)Phenolic CompoundThe objective of this study was to carry out phytochemical analysis of the bark extract of Casearia javitensis and to evaluate its antipromastigote activity against Leishmania amazonensis. The ethanol extract (EE) was fractionated in open-column chromatography to obtain the hexane (FrHex), dichloromethane (FrDcm), ethyl acetate (FrAcOET) and methanol (FrMeOH) fractions. The EE and its fractions were analyzed by thin layer chromatography (TLC). FrDcm was analyzed in high performance liquid chromatography with diode array detection (HPLC-DAD). The antipromastigote assay of Leishmania amazonensis and the cytotoxicity test against the acute monocytic leukemia cell line (THP-1) were performed by the MTT colorimetric assay. The phytochemical profile in TLC detected terpenes in all samples. However, in the ultraviolet spectrum (HPLC-DAD) for FrDcm suggested the presence of a phenolic compound. FrHex and FrDcm showed antipromastigote activity (IC50 = 116.6 ± 0.9 and 59.4 ± 1.1 μg mL-1, respectively) and low cytotoxicity (CC50 = 333.4 ± 3.2 and 241.2 ± 1.9 μg mL-1, respectively). The selectivity index for FrDcm was 4.1. We conclude that the FrDcm of C. javitensis has potantial as a leishmanicide, and this activity may be related to the presence of phenolic compound.O objetivo deste trabalho foi realizar estudos fitoquímicos e avaliar a atividade antipromastigota (Leishmania amazonensis) da casca de Casearia javitensis. O extrato etanólico (EE) foi obtido através da maceração do pó da casca com etanol. O extrato foi fracionado em coluna cromatografia aberta, originando as frações hexânica (FrHex), diclorometânica (FrDcm), acetato de etila (FrAcOET) e metanólica (FrMeOH). O EE e suas frações foram analisados por cromatografia em camada delgada (CCD), e a FrDcm em cromatografia líquida de alta eficiência acoplada ao detector de arranjo de diodos (CLAE-DAD). O ensaio antipromastigota e citotoxicidade frente à linhagem celular de leucemia monocítica aguda (THP-1) foram realizados pelo teste colorimétrico MTT. No perfil fitoquímico em CCD do EE e frações FrHex, FrAcOET e FrMeOH foram detectados terpenos e na FrDcm em espectro em ultravioleta (CLAE-DAD), sugere-se tratar de um composto fenólico. As frações FrHex (CI50 = 116,6 ± 0,9 μg mL-1) e FrDcm (CI50 = 59,38 ± 1,1 μg mL-1) foram ativas em promastigota e apresentaram baixa citotoxicidade (CC50 = 333,4 ± 3,2 e 241,2 ± 1,9 μg mL-1, respectivamente), sendo FrDcm o índice de seletividade de 4,1. Portanto, FrDcm mostrou-se promissora como leishmanicida e essa atividade pode estar relacionada a um composto fenólico.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2020-12-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/95010.32712/2446-4775.2020.950Revista Fitos; Vol. 14 No. 4 (2020); 528-537Revista Fitos; Vol. 14 Núm. 4 (2020); 528-537Revista Fitos; v. 14 n. 4 (2020); 528-5372446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950/749https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950/750Copyright (c) 2020 Revista Fitoshttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess Silva, Carolina Moraes daEiró, Luciana GuimarãesSilva-Silva, João Victor Corrêa-Barbosa, JulianaCoelho-Ferreira, Márlia ReginaSilveira, Fernando TobiasMarinho, Andrey Moacir do RosárioAlmeida, Maria das Graças Carvalho Brigido, Heliton Patrick CordovilDolabela, Maria Fâni 2020-12-18T10:45:49Zoai:ojs.revistafitos.far.fiocruz.br:article/950Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2020-12-18T10:45:49Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.none.fl_str_mv |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) Atividade antileishmanial in vitro e análise fitoquímica de Casearia javitensis Kunth (Salicaceae) |
| title |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) |
| spellingShingle |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) Silva, Carolina Moraes da Leishmaniose Antripromastigota Citotoxicidade (THP-1) Composto Fenólico Leismaniasis Antipromastigote Cytotoxicity (THP-1) Phenolic Compound |
| title_short |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) |
| title_full |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) |
| title_fullStr |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) |
| title_full_unstemmed |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) |
| title_sort |
In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae) |
| author |
Silva, Carolina Moraes da |
| author_facet |
Silva, Carolina Moraes da Eiró, Luciana Guimarães Silva-Silva, João Victor Corrêa-Barbosa, Juliana Coelho-Ferreira, Márlia Regina Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosário Almeida, Maria das Graças Carvalho Brigido, Heliton Patrick Cordovil Dolabela, Maria Fâni |
| author_role |
author |
| author2 |
Eiró, Luciana Guimarães Silva-Silva, João Victor Corrêa-Barbosa, Juliana Coelho-Ferreira, Márlia Regina Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosário Almeida, Maria das Graças Carvalho Brigido, Heliton Patrick Cordovil Dolabela, Maria Fâni |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Carolina Moraes da Eiró, Luciana Guimarães Silva-Silva, João Victor Corrêa-Barbosa, Juliana Coelho-Ferreira, Márlia Regina Silveira, Fernando Tobias Marinho, Andrey Moacir do Rosário Almeida, Maria das Graças Carvalho Brigido, Heliton Patrick Cordovil Dolabela, Maria Fâni |
| dc.subject.por.fl_str_mv |
Leishmaniose Antripromastigota Citotoxicidade (THP-1) Composto Fenólico Leismaniasis Antipromastigote Cytotoxicity (THP-1) Phenolic Compound |
| topic |
Leishmaniose Antripromastigota Citotoxicidade (THP-1) Composto Fenólico Leismaniasis Antipromastigote Cytotoxicity (THP-1) Phenolic Compound |
| description |
The objective of this study was to carry out phytochemical analysis of the bark extract of Casearia javitensis and to evaluate its antipromastigote activity against Leishmania amazonensis. The ethanol extract (EE) was fractionated in open-column chromatography to obtain the hexane (FrHex), dichloromethane (FrDcm), ethyl acetate (FrAcOET) and methanol (FrMeOH) fractions. The EE and its fractions were analyzed by thin layer chromatography (TLC). FrDcm was analyzed in high performance liquid chromatography with diode array detection (HPLC-DAD). The antipromastigote assay of Leishmania amazonensis and the cytotoxicity test against the acute monocytic leukemia cell line (THP-1) were performed by the MTT colorimetric assay. The phytochemical profile in TLC detected terpenes in all samples. However, in the ultraviolet spectrum (HPLC-DAD) for FrDcm suggested the presence of a phenolic compound. FrHex and FrDcm showed antipromastigote activity (IC50 = 116.6 ± 0.9 and 59.4 ± 1.1 μg mL-1, respectively) and low cytotoxicity (CC50 = 333.4 ± 3.2 and 241.2 ± 1.9 μg mL-1, respectively). The selectivity index for FrDcm was 4.1. We conclude that the FrDcm of C. javitensis has potantial as a leishmanicide, and this activity may be related to the presence of phenolic compound. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-18 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950 10.32712/2446-4775.2020.950 |
| url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950 |
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10.32712/2446-4775.2020.950 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950/749 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950/750 |
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Copyright (c) 2020 Revista Fitos https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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Copyright (c) 2020 Revista Fitos https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
| publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
| dc.source.none.fl_str_mv |
Revista Fitos; Vol. 14 No. 4 (2020); 528-537 Revista Fitos; Vol. 14 Núm. 4 (2020); 528-537 Revista Fitos; v. 14 n. 4 (2020); 528-537 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Revista Fitos |
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Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
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