Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively

Detalhes bibliográficos
Autor(a) principal: Tavares, E. S.
Data de Publicação: 2005
Outros Autores: Leitão, S. G., Reinert, F., Lage, C. L. S.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Fitos
Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17
Resumo: This work describes a micropropagation protocol for three different chemotypes of Lippia alba, which essential oil’s major components are citral, carvone and linalol. L. alba is widely used in traditional medicine and among other characteristics, is of great economical interest for its essential oils. Nodal segments of three chemotypes, were cultured in vitro. Plants were grown in Murashigue and Skoog (MS) medium supplemented with sucrose and vitamins but not hormones, with and without norbixin. Plants obtained in this way were then subcultured to identify if the addition of IAA and kinetin optimized plant growth. The results showed that these hormones were not required to successfully culture and multiply the three chemotypes of L. alba in vitro. Addition of norbixin was efficient in decreasing plant tissue oxidation. This faster and more economical protocol did not affect ex vitro acclimation of plants that was also successful.
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spelling Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool RespectivelyMicropropagação de Quimiotipos de Lippia Alba Mill. N. E. Br Produtores de Citral, Carvona e Linalol.Lippia albaVerbenaceaecultura de tecidoscitralcarvonalinalolLippia albaVerbenaceaetissue culturecitralcarvonelinalolThis work describes a micropropagation protocol for three different chemotypes of Lippia alba, which essential oil’s major components are citral, carvone and linalol. L. alba is widely used in traditional medicine and among other characteristics, is of great economical interest for its essential oils. Nodal segments of three chemotypes, were cultured in vitro. Plants were grown in Murashigue and Skoog (MS) medium supplemented with sucrose and vitamins but not hormones, with and without norbixin. Plants obtained in this way were then subcultured to identify if the addition of IAA and kinetin optimized plant growth. The results showed that these hormones were not required to successfully culture and multiply the three chemotypes of L. alba in vitro. Addition of norbixin was efficient in decreasing plant tissue oxidation. This faster and more economical protocol did not affect ex vitro acclimation of plants that was also successful.O trabalho descreve um protocolo de micropropagação de três quimiotipos de Lippia alba, produtores de citral, carvona e linalol como componentes majoritários do óleo essencial. A espécie é amplamente utilizada na medicina tradicional e de grande interesse econômico devido, dentre outros fatores, às propriedades dos componentes majoritários do óleo essencial. Segmentos nodais de ramos dos três quimiotipos, foram introduzidos in vitro. Foi utilizado o meio de cultura Murashigue e Skoog, suplementado com sacarose e vitaminas, e desprovido de hormônios (MS), com e sem adição de norbixina. As plantas obtidas foram subcultivadas com concentrações variadas de AIA (ácido indolacético) e cinetina. A adição de norbixina foi eficaz em diminuir a morte dos explantes por oxidação. Os hormônios testados não são necessários para a multiplicação dos três quimiotipos de L. alba in vitro. Este protocolo mais rápido e econômico não afetou a aclimatação das plantasmicropropagadas, a qual foi obtida com sucesso.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2005-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1710.32712/2446-4775.2005.17Revista Fitos; Vol. 1 No. 01 (2005); 67-73Revista Fitos; Vol. 1 Núm. 01 (2005); 67-73Revista Fitos; v. 1 n. 01 (2005); 67-732446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17/pdf_9https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17/1307Copyright (c) 2005 Revista Fitos Eletrônicahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTavares, E. S.Leitão, S. G.Reinert, F.Lage, C. L. S.2022-11-07T14:46:03Zoai:ojs.revistafitos.far.fiocruz.br:article/17Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2022-11-07T14:46:03Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
Micropropagação de Quimiotipos de Lippia Alba Mill. N. E. Br Produtores de Citral, Carvona e Linalol.
title Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
spellingShingle Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
Tavares, E. S.
Lippia alba
Verbenaceae
cultura de tecidos
citral
carvona
linalol
Lippia alba
Verbenaceae
tissue culture
citral
carvone
linalol
title_short Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
title_full Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
title_fullStr Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
title_full_unstemmed Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
title_sort Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively
author Tavares, E. S.
author_facet Tavares, E. S.
Leitão, S. G.
Reinert, F.
Lage, C. L. S.
author_role author
author2 Leitão, S. G.
Reinert, F.
Lage, C. L. S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Tavares, E. S.
Leitão, S. G.
Reinert, F.
Lage, C. L. S.
dc.subject.por.fl_str_mv Lippia alba
Verbenaceae
cultura de tecidos
citral
carvona
linalol
Lippia alba
Verbenaceae
tissue culture
citral
carvone
linalol
topic Lippia alba
Verbenaceae
cultura de tecidos
citral
carvona
linalol
Lippia alba
Verbenaceae
tissue culture
citral
carvone
linalol
description This work describes a micropropagation protocol for three different chemotypes of Lippia alba, which essential oil’s major components are citral, carvone and linalol. L. alba is widely used in traditional medicine and among other characteristics, is of great economical interest for its essential oils. Nodal segments of three chemotypes, were cultured in vitro. Plants were grown in Murashigue and Skoog (MS) medium supplemented with sucrose and vitamins but not hormones, with and without norbixin. Plants obtained in this way were then subcultured to identify if the addition of IAA and kinetin optimized plant growth. The results showed that these hormones were not required to successfully culture and multiply the three chemotypes of L. alba in vitro. Addition of norbixin was efficient in decreasing plant tissue oxidation. This faster and more economical protocol did not affect ex vitro acclimation of plants that was also successful.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17
10.32712/2446-4775.2005.17
url https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17
identifier_str_mv 10.32712/2446-4775.2005.17
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17/pdf_9
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17/1307
dc.rights.driver.fl_str_mv Copyright (c) 2005 Revista Fitos Eletrônica
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2005 Revista Fitos Eletrônica
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
dc.source.none.fl_str_mv Revista Fitos; Vol. 1 No. 01 (2005); 67-73
Revista Fitos; Vol. 1 Núm. 01 (2005); 67-73
Revista Fitos; v. 1 n. 01 (2005); 67-73
2446-4775
1808-9569
reponame:Revista Fitos
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Revista Fitos
collection Revista Fitos
repository.name.fl_str_mv Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br
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