Influence of seasonality on flavonoid content, antioxidant potential and toxicity of Doliocarpus dentatus leaf infusion

• Autor(a) principal: Gaiola, Letícia
• Data de Publicação: 2022
• Outros Autores: Cardoso, Claudia Andrea Lima
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1164
• topic: Sazonalidade. Flavonoides. Saponinas. Antioxidante. DPPH. Cromatografia. Toxicidade. Artemia.; Seasonality. Flavonoids. Saponins. Antioxidant. DPPH. Chromatography. Toxicity. Artemia.

The Doliocarpus dentatus (Aubl.) Standl. is a plant used in treatment of several health problems, however, there are no studies that assess the influence of seasonality on toxicity and on the chemical composition of the infusion this species leaves. Thus, the present study aimed to evaluate the influence of seasonality on toxicity, chemical composition and antioxidant activity of D. dentatus leaf infusion. Yield (EAJAN19) in summer (EAJAN19), autumn (EAMAI18), winter (EAJUL18) and spring (EASET18), were 35.80%; 37.27%; 33.55%; 32.80% respectively. The levels of flavonoids were higher in EAMAI18 (7.20 ± 0.21 mg g-1) and EAJAN19 (6.76 ± 0.06 mg g-1). In the analysis of antioxidant activity, the IC50 values ​​were EAJUL18 (5.50 ± 0.07 µg mL-1), followed by EASET18 (3.72 ± 0.28 µg mL-1), EAJAN19 (2.78 ± 0.31 µg mL-1) and EAMAI18 (1.24 ± 0.54 µg mL-1). For the different seasons, a direct relationship between flavonoid contents and antioxidant activity was observed. In the toxicity test with Artemia salina, as founded in summer, autumn, winter and spring, independent LD50 of 3.48 ± 0.11 mg mL-1; 3.34 ± 0.13 mg mL-1; 3.67 ± 0.11 mg mL-1 and 3.58 ± 0.13 mg mL-1, respectively, showing no toxicity.