Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response

Detalhes bibliográficos
Autor(a) principal: Fonseca,Gabriela Rodrigues e
Data de Publicação: 2017
Outros Autores: Santos,Sergio Vieira dos, Chieffi,Pedro Paulo, Paula,Fabiana Martins de, Gryschek,Ronaldo Cesar Borges, Lescano,Susana Angélica Zevallos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382
Resumo: BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.
id FIOCRUZ-4_1029567873d903684b916ef3fb214529
oai_identifier_str oai:scielo:S0074-02762017000500382
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune responseToxocara canisBALB/cELISAlarval recoveryBALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.Instituto Oswaldo Cruz, Ministério da Saúde2017-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382Memórias do Instituto Oswaldo Cruz v.112 n.5 2017reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760160341info:eu-repo/semantics/openAccessFonseca,Gabriela Rodrigues eSantos,Sergio Vieira dosChieffi,Pedro PauloPaula,Fabiana Martins deGryschek,Ronaldo Cesar BorgesLescano,Susana Angélica Zevalloseng2020-04-25T17:52:41Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:58.94Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
title Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
spellingShingle Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
Fonseca,Gabriela Rodrigues e
Toxocara canis
BALB/c
ELISA
larval recovery
title_short Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
title_full Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
title_fullStr Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
title_full_unstemmed Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
title_sort Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
author Fonseca,Gabriela Rodrigues e
author_facet Fonseca,Gabriela Rodrigues e
Santos,Sergio Vieira dos
Chieffi,Pedro Paulo
Paula,Fabiana Martins de
Gryschek,Ronaldo Cesar Borges
Lescano,Susana Angélica Zevallos
author_role author
author2 Santos,Sergio Vieira dos
Chieffi,Pedro Paulo
Paula,Fabiana Martins de
Gryschek,Ronaldo Cesar Borges
Lescano,Susana Angélica Zevallos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca,Gabriela Rodrigues e
Santos,Sergio Vieira dos
Chieffi,Pedro Paulo
Paula,Fabiana Martins de
Gryschek,Ronaldo Cesar Borges
Lescano,Susana Angélica Zevallos
dc.subject.por.fl_str_mv Toxocara canis
BALB/c
ELISA
larval recovery
topic Toxocara canis
BALB/c
ELISA
larval recovery
dc.description.none.fl_txt_mv BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.
description BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760160341
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.112 n.5 2017
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937724180660224