Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382 |
Resumo: | BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain. |
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Memórias do Instituto Oswaldo Cruz |
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Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune responseToxocara canisBALB/cELISAlarval recoveryBALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.Instituto Oswaldo Cruz, Ministério da Saúde2017-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382Memórias do Instituto Oswaldo Cruz v.112 n.5 2017reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760160341info:eu-repo/semantics/openAccessFonseca,Gabriela Rodrigues eSantos,Sergio Vieira dosChieffi,Pedro PauloPaula,Fabiana Martins deGryschek,Ronaldo Cesar BorgesLescano,Susana Angélica Zevalloseng2020-04-25T17:52:41Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:21:58.94Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
title |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
spellingShingle |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response Fonseca,Gabriela Rodrigues e Toxocara canis BALB/c ELISA larval recovery |
title_short |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
title_full |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
title_fullStr |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
title_full_unstemmed |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
title_sort |
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response |
author |
Fonseca,Gabriela Rodrigues e |
author_facet |
Fonseca,Gabriela Rodrigues e Santos,Sergio Vieira dos Chieffi,Pedro Paulo Paula,Fabiana Martins de Gryschek,Ronaldo Cesar Borges Lescano,Susana Angélica Zevallos |
author_role |
author |
author2 |
Santos,Sergio Vieira dos Chieffi,Pedro Paulo Paula,Fabiana Martins de Gryschek,Ronaldo Cesar Borges Lescano,Susana Angélica Zevallos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca,Gabriela Rodrigues e Santos,Sergio Vieira dos Chieffi,Pedro Paulo Paula,Fabiana Martins de Gryschek,Ronaldo Cesar Borges Lescano,Susana Angélica Zevallos |
dc.subject.por.fl_str_mv |
Toxocara canis BALB/c ELISA larval recovery |
topic |
Toxocara canis BALB/c ELISA larval recovery |
dc.description.none.fl_txt_mv |
BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain. |
description |
BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500382 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760160341 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.112 n.5 2017 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937724180660224 |